1. p416 103Q GAL

   Plasmid

   #1186

   Depositor

   Susan Lindquist

   Article

   Krobitsch et al Proc Natl Acad Sci U S A 2000 Feb 15;97(

   Insert

   htt 103Q (HTT Human)
   

   Use

   Tags

   GFP

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   May 25, 2005

   Availability

   Academic Institutions and Nonprofits only

   

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These results may also be relevant.

1. pSYC-190

   Plasmid

   #178962

   Purpose

   Expresses human GFAP (R79C) mutant fused to 3xFLAG tag in mammalian cells

   Depositor

   Seok-Yong Choi

   Article

   Lee et al BMC Neurol. 2017 Sep 7;17(1):175. doi: 1

   Insert

   Glial fibrillary acidic protein (GFAP Human)
   

   Use

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   R79C

   Promoter

   CMV IE94 Promoter

   Available since

   March 11, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pEJS654 All-in-One AAV-sgRNA-hNmeCas9

   Plasmid

   #112139

   Purpose

   Delivery of human-codon-optimized Cas9 from Neisseria meningitidis (NmeCas9) and its single-guide RNA in a single AAV vector for in vivo genome editing.

   Depositor

   Erik Sontheimer

   Article

   Ibraheim et al Genome Biol. 2018 Sep 19;19(1):137. doi:

   Inserts

   sgRNA scaffold
   Human-codon-optimized NmeCas9
   

   Use

   AAV, CRISPR, and Mouse Targeting

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U1a and U6

   Available since

   Aug. 13, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. MSCVpic2neo-hTERT-p16 shRNA

   Plasmid

   #164920

   Purpose

   Expresses hTERT cDNA and p16 shRNA in mammalian cells

   Depositor

   Ji Luo

   Article

   Smith et al Cell Biosci. 2016 Nov 11;6:57. doi: 10.1

   Inserts

   human telomerase reverse transcriptase (TERT Human)
   CDKN2A (CDKN2A Human)
   

   Use

   Retroviral

   Tags

   HA tag

   Expression

   Mutation

   Promoter

   Available since

   May 26, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. SITS-mCherry-Bin1b

   Plasmid

   #176025

   Purpose

   Leishmania cell free expression of zebrafish mCherry-Bin1b. Parton lab clone GSU

   Depositor

   Rob Parton

   Article

   Lo et al J Cell Biol. 2021 Dec 6;220(12). pii: 21

   Insert

   Bin1b (bin1b Zebrafish)
   

   Use

   Leishmania cell free expression

   Tags

   mCherry

   Expression

   Mutation

   Promoter

   Available since

   Nov. 4, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAAV-C2m2-SNAP

   Plasmid

   #208791

   Purpose

   For AAV production to express C2m2-SNAP in astrocytes

   Depositor

   Urte Neniskyte

   Article

   Dirvelyte et al Cell Mol Biol Lett. 2023 Jul 27;28(1):59

   Insert

   C2m2-SNAP (Mfge8 Mouse)
   

   Use

   AAV

   Tags

   SNAP-tag

   Expression

   Mammalian

   Mutation

   Mutated 24 and 45 lysine to asparagine in C2 doma…

   Promoter

   GFAP promoter

   Available since

   Nov. 7, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. FLAG-LoxP-PURO-LoxP-SNAP-TERT

   Plasmid

   #71390

   Purpose

   Homologues recombination donor plasmid for inserting a FLAG-SNAP-tag at the N-terminus of the TERT locus in human cells. Includes PURO resistance flanked by LoxP sites for selection.

   Depositor

   Thomas Cech

   Article

   Xi et al Genome Biol. 2015 Nov 10;16(1):231. doi:

   Insert

   TERT (TERT Human)
   

   Use

   Hr donor

   Tags

   FLAG-SNAP

   Expression

   Mutation

   Promoter

   Endogenous TERT promotor

   Available since

   Dec. 10, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAAV-C2m2-mKate

   Plasmid

   #208793

   Purpose

   For AAV production to express C2m2-mKate in astrocytes

   Depositor

   Urte Neniskyte

   Article

   Dirvelyte et al Cell Mol Biol Lett. 2023 Jul 27;28(1):59

   Insert

   C2m2-mKate (Mfge8 Mouse)
   

   Use

   AAV

   Tags

   mKate

   Expression

   Mammalian

   Mutation

   Mutated 24 and 45 lysine to asparagine in C2 doma…

   Promoter

   GFAP promoter

   Available since

   Nov. 7, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pUB6-HuMGF

   Plasmid

   #190782

   Purpose

   Expresses membrane-bound form of human SCF in mammalian cells

   Depositor

   Jakob Reiser

   Article

   Zhang et al Retrovirology. 2010 Jan 25;7:3. doi: 10.

   Insert

   human stem cell factor (KITLG Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   UbC

   Available since

   Nov. 10, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. H513 px330.sgActin.UTR.2

   Plasmid

   #138176

   Purpose

   CRISPR SONIC: Encodes codon-optimized SpCas9 and gRNA for targeting 3' UTR of β-actin.

   Depositor

   Wen Xue

   Article

   Mou et al Genome Med. 2019 Apr 16;11(1):21. doi: 1

   Insert

   sgActin (Actb Mouse)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   March 18, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. tRNA-gRNA position [M1_n-1] (GB1210)

    Plasmid

    #75411

    Purpose

    tRNA and scaffold for the assembly of GBoligomers for the first position (positon [M1_n-1]) of a polycistronic tRNA-gRNA regulated by the (monocot) U3 promoter (2-part multiplexing)

    Depositor

    Diego Orzaez

    Article

    Vazquez-Vilar et al Plant Methods. 2016 Feb 1;12:10. doi: 10

    Insert

    tRNA-gRNA position [M1_n-1]
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Aug. 5, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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11. pEGB2Ω2_SF-35s:hCas9:tNos (GB1103)

    Plasmid

    #75400

    Purpose

    Transcriptional unit for (human codon optimized) Cas9 plant expression driven by the 35S promoter. Specially conceived to be linked with the TU of the kanamycin resistance gene GB1181.

    Depositor

    Diego Orzaez

    Article

    Vazquez-Vilar et al Plant Methods. 2016 Feb 1;12:10. doi: 10

    Insert

    hCas9
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Plant

    Mutation

    Promoter

    35S

    Available since

    Aug. 5, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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12. pEGB SF-35S:Renilla:TNOS-35S:P19:TNOS (GB0160)

    Plasmid

    #75412

    Purpose

    Module for the expression of the Renilla Luciferase with the silencing suppressor P19

    Depositor

    Diego Orzaez

    Article

    Vazquez-Vilar et al Plant Methods. 2016 Feb 1;12:10. doi: 10

    Insert

    Renilla / P19
    

    Use

    Luciferase and Synthetic Biology

    Tags

    Expression

    Plant

    Mutation

    Promoter

    35S

    Available since

    Aug. 5, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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13. pZE21/UBP1/ClpS

    Plasmid

    #98566

    Purpose

    Expresses truncated codon-opt S. cerevisiae UBP1 and E. coli ClpS in an artificial operon

    Depositor

    George Church

    Article

    Kunjapur et al Proc Natl Acad Sci U S A. 2018 Jan 16;11

    Inserts

    Truncated ubiquitin cleavase, codon optimized
    ATP-dependent Clp protease adapter protein
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    In a synthetic operon downstream of UBP1 and Remo…

    Promoter

    Same as UBP1 (operon) and Tet promoter (aTc induc…

    Available since

    Feb. 7, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. NLS-HA-2xMCP-Ezh2

    Plasmid

    #126590

    Purpose

    Expresses MCP (MS2 Coat Protein) fusion to Ezh2 in mammalian cells, lentiviral backbone

    Depositor

    David Segal

    Article

    O'Geen et al Epigenetics Chromatin. 2019 May 3;12(1):

    Insert

    2XMCP-Ezh2 (Ezh2 Synthetic, Mouse)
    

    Use

    Lentiviral

    Tags

    NLS-HA

    Expression

    Mammalian

    Mutation

    Promoter

    human ubiquitin C promoter

    Available since

    May 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pUDP044

    Plasmid

    #101168

    Purpose

    pUDP004 expressing a polycistronic g-RNA array for Cas9 editing targeting the genes SeATF1 and SeATF2 and Spcas9D147Y P411T in S. pastorianus (HH-gRNASeATF1-HDV-linker-HH-gRNASeATF2-HDV)

    Depositor

    Jean-Marc Daran

    Article

    de Vries et al Microb Cell Fact. 2017 Dec 5;16(1):222.

    Insert

    polycistronic HH-gRNA-HDV-HH-gRNA-HDV array targetting SeATF1 and 2 in S. pastorianus
    

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    ScTDH3

    Available since

    Dec. 4, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. pPICZ-pre-Ost1-alphaf(I)-Btl2

    Plasmid

    #117667

    Purpose

    Study secretion efficiency of Btl2

    Depositor

    Pau Ferrer, Benjamin Glick

    Article

    Barrero et al Microb Cell Fact. 2018 Oct 12;17(1):161.

    Insert

    pre-Ost1-alphaf(I)-Btl2
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    This plasmid encodes the lipase Btl2 together wit…

    Promoter

    pAOX1

    Available since

    Dec. 12, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. pcDNA3-Flag-NSF/S647A

    Plasmid

    #74938

    Purpose

    Mammalian expression of human NSF mutant S647A (mutation in the D2 domain of the protein)

    Depositor

    Elisa Greggio

    Article

    Belluzzi et al Mol Neurodegener. 2016 Jan 13;11(1):1. d

    Insert

    NSF (NSF Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    S647A (mutation in the D2 domain of the protein)

    Promoter

    CMV

    Available since

    April 20, 2016

    Availability

    Academic Institutions and Nonprofits only

18. pOTTC1663 - pAAV SYN1 mGas6-Myc-DDK

    Plasmid

    #202540

    Purpose

    An adeno-associated viral vector expressing murine Gas6 fused Myc and DDK epitopes from a synapsin promoter

    Depositor

    Christopher Richie

    Article

    Owlett et al J Neuroinflammation. 2022 Feb 7;19(1):38

    Insert

    Gas6 (Gas6 Mouse)
    

    Use

    AAV

    Tags

    Myc-DDK

    Expression

    Mutation

    Promoter

    SYN1

    Available since

    June 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. pSUMO His6 SUMO SnRK2.6 (11-362)

    Plasmid

    #177847

    Purpose

    Bacterial Expression of SnRK2.6

    Depositor

    Karsten Melcher

    Article

    Ng et al Proc Natl Acad Sci U S A. 2011 Dec 27;10

    Insert

    SNF1-related protein kinase 2.6 (OST1 Mustard Weed)
    

    Use

    Tags

    6xHis-SUMO

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Dec. 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. SITS-EGFP-Bin1b

    Plasmid

    #176021

    Purpose

    Leishmania cell free expression of zebrafish EGFP-Bin1b. Parton lab clone GRS

    Depositor

    Rob Parton

    Article

    Lo et al J Cell Biol. 2021 Dec 6;220(12). pii: 21

    Insert

    Bin1b (bin1b Zebrafish)
    

    Use

    Leishmania cell free expression

    Tags

    EGFP

    Expression

    Mutation

    Promoter

    Available since

    Nov. 4, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge