1. p416 103Q GAL

   Plasmid

   #1186

   Depositor

   Susan Lindquist

   Article

   Krobitsch et al Proc Natl Acad Sci U S A 2000 Feb 15;97(

   Insert

   htt 103Q (HTT Human)
   

   Use

   Tags

   GFP

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

These results may also be relevant.

1. pLenti-PGK-Neo-PIP-FUCCI

   Plasmid

   #118616

   Purpose

   Lentiviral expression of the cell cycle marker PIP-FUCCI

   Depositor

   Jean Cook

   Article

   Grant et al Cell Cycle. 2018;17(21-22):2496-2516. do

   Insert

   PIP-FUCCI
   

   Use

   Lentiviral

   Tags

   mCherry and mVenus

   Expression

   Mammalian

   Mutation

   Promoter

   hPGK

   Available since

   May 20, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pHAGE_puro

   Plasmid

   #118692

   Purpose

   Empty vector to express overexpression cDNA

   Depositor

   Christopher Vakoc

   Article

   Lu et al Cancer Cell. 2018 Dec 10;34(6):970-981.e

   Type

   Empty backbone

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   CMV

   Available since

   Dec. 7, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pMP71-hPDL-1

   Plasmid

   #118631

   Purpose

   human PDL-1 coding sequence subcloned into pMP71 retroviral plasmid

   Depositor

   Sébastien Walchli

   Article

   Josefsson et al Cancer Immunol Res. 2019 Mar;7(3):355-36

   Insert

   hPDL-1 (CD274 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   LTR

   Available since

   May 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pLenti-PGK-Neo-PIP-NLS-mVenus

   Plasmid

   #118619

   Purpose

   Lentiviral expression of the cell cycle marker PIP-NLS-mVenus

   Depositor

   Jean Cook

   Article

   Grant et al Cell Cycle. 2018;17(21-22):2496-2516. do

   Insert

   PIP-NLS-mVenus
   

   Use

   Lentiviral

   Tags

   mVenus

   Expression

   Mammalian

   Mutation

   Promoter

   hPGK

   Available since

   May 20, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pLenti-PGK-Puro-TK-NLS-mTurq2-PCNA

   Plasmid

   #118617

   Purpose

   Lentiviral expression of mTurq2 tagged PCNA

   Depositor

   Jean Cook

   Article

   Grant et al Cell Cycle. 2018;17(21-22):2496-2516. do

   Insert

   TK-NLS-mTurq2-PCNA (PCNA Human)
   

   Use

   Lentiviral

   Tags

   mTurq2

   Expression

   Mammalian

   Mutation

   Promoter

   hPGK

   Available since

   May 9, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pAC1802_pmax-RBFOX1N-dCasRx-C

   Plasmid

   #118635

   Purpose

   Transient transfection; Expresses RBFOX1N-dCasRx-C; CAGGS promoter

   Depositor

   Albert Cheng

   Article

   Du et al Nat Commun. 2020 Jun 12;11(1):2973. doi:

   Insert

   RBFOX1N-dCasRx-C
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAGGS

   Available since

   Jan. 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pAC1821_pCR8-SMN2-DN-RG6-SA

   Plasmid

   #118655

   Purpose

   Transient transfection; Expressed DR-SMN2-DN-RG6-SA-DR CasRx polycistronic gRNA with 3 spacers targeting SMN2 intron and 1 spacer targeting RG6 splice acceptor; Gateway Donor

   Depositor

   Albert Cheng

   Article

   Du et al Nat Commun. 2020 Jun 12;11(1):2973. doi:

   Insert

   SMN2-DN-RG6-SA
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Jan. 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. Lenti_MS2_P65_HSF1_2A_puro

   Plasmid

   #118699

   Purpose

   To express MS2-P65-HSF1

   Depositor

   Christopher Vakoc

   Article

   Lu et al Cancer Cell. 2018 Dec 10;34(6):970-981.e

   Type

   Empty backbone

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Jan. 9, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. Lenti_sgRNA_MS2_neo

   Plasmid

   #118650

   Purpose

   To clone sgRNA for activation dCas9

   Depositor

   Christopher Vakoc

   Article

   Lu et al Cancer Cell. 2018 Dec 10;34(6):970-981.e

   Type

   Empty backbone

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   U6

   Available since

   Jan. 9, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pAC1801_pmax-dCasRx

    Plasmid

    #118634

    Purpose

    Transient transfection; Expresses dCasRx; CAGGS promoter

    Depositor

    Albert Cheng

    Article

    Du et al Nat Commun. 2020 Jun 12;11(1):2973. doi:

    Insert

    dCasRx
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    R239A/H244A/R858A/H863A

    Promoter

    CAGGS

    Available since

    Jan. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. HF-PX459 (V2)

    Plasmid

    #118632

    Purpose

    Plasmid encoding SpCas9-HF1, a single guide RNA and puromycin resistance

    Depositor

    Mike McGrew

    Article

    Idoko-Akoh et al Sci Rep. 2018 Oct 11;8(1):15126. doi: 10

    Insert

    hSpCas9-HF1-2A-Puro V2.0
    

    Use

    CRISPR

    Tags

    2A-Puro and 3XFLAG

    Expression

    Mammalian

    Mutation

    Asparagine 497 to Alanine, Arginine 661 to Alanin…

    Promoter

    Cbh

    Available since

    Dec. 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pENTR-PIP-FUCCI

    Plasmid

    #118621

    Purpose

    Gateway cloning vector for the cell cycle indicator PIP-FUCCI

    Depositor

    Jean Cook

    Article

    Grant et al Cell Cycle. 2018;17(21-22):2496-2516. do

    Insert

    PIP-FUCCI
    

    Use

    Gateway cloning

    Tags

    mCherry and mVenus

    Expression

    Mutation

    Promoter

    Available since

    April 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. Dead-Laconic/pcDNA3.1(-)

    Plasmid

    #118627

    Purpose

    To express in mammalian cells a lactate FRET sensor negative control.

    Depositor

    Luis Felipe Barros

    Article

    Baeza-Lehnert et al Methods Mol Biol. 2020;2184:19-30. doi:

    Insert

    Dead-Laconic
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Change Histidine 151 to Aspartic Acid of Escheric…

    Promoter

    CMV Promoter

    Available since

    Dec. 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. Lenti_SaCRISPR_GFP

    Plasmid

    #118636

    Purpose

    To clone sgRNA for SaCas9 (SauCas9)

    Depositor

    Christopher Vakoc

    Article

    Lu et al Cancer Cell. 2018 Dec 10;34(6):970-981.e

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    U6

    Available since

    Jan. 9, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pLX304 ERG

    Plasmid

    #118625

    Purpose

    Overexpresses ERG protein

    Depositor

    William Hahn

    Article

    Sandoval et al Mol Cell. 2018 Aug 16;71(4):554-566.e7.

    Insert

    ERG (33-479) (ERG Human)
    

    Use

    Lentiviral

    Tags

    None and none

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 26, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pMP71-hCD155

    Plasmid

    #118630

    Purpose

    human CD155 coding sequence subcloned into pMP71 retroviral plasmid

    Depositor

    Sébastien Walchli

    Article

    Josefsson et al Cancer Immunol Res. 2019 Mar;7(3):355-36

    Insert

    CD155 (PVR Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    LTR

    Available since

    May 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pAC1817_pCR8-gSMN2-EX

    Plasmid

    #118651

    Purpose

    Transient transfection; Expressed SMN2-EX CasRx gRNA; Gateway Donor

    Depositor

    Albert Cheng

    Article

    Du et al Nat Commun. 2020 Jun 12;11(1):2973. doi:

    Insert

    gSMN2-EX
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Jan. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pAC1816_pCR8-gSMN2-DN4

    Plasmid

    #118649

    Purpose

    Transient transfection; Expressed SMN2-DN4 CasRx gRNA; Gateway Donor

    Depositor

    Albert Cheng

    Article

    Du et al Nat Commun. 2020 Jun 12;11(1):2973. doi:

    Insert

    gSMN2-DN4
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Jan. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pLX304 ERG R367K

    Plasmid

    #118626

    Purpose

    Overexpresses ERG R367K (DNA binding mutant) protein

    Depositor

    William Hahn

    Article

    Sandoval et al Mol Cell. 2018 Aug 16;71(4):554-566.e7.

    Insert

    ERG ( R367K DNA Binding mutant) (ERG Human)
    

    Use

    Lentiviral

    Tags

    None

    Expression

    Mammalian

    Mutation

    R367K

    Promoter

    CMV

    Available since

    Nov. 26, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. V5-ERG

    Plasmid

    #118623

    Purpose

    Expresses V5 tagged ERG protein

    Depositor

    William Hahn

    Article

    Sandoval et al Mol Cell. 2018 Aug 16;71(4):554-566.e7.

    Insert

    ERG (ERG Human)
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Dec. 3, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge