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Lentiviral mBmal1-Luc

Plasmid

#182762

Purpose

Lentiviral vector encoding the promoter region of mouse Bmal1 gene driving a luciferase reporter

Depositor

Qing-Jun Meng

Article

Gossan et al Arthritis Rheum. 2013 Sep;65(9):2334-45.

Insert

mouse arntl promoter (Bmal1 Mouse)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

649 bp

Available since

Sept. 8, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA 3.2/V5-DEST MTFR1L-V5

Plasmid

#69822

Purpose

Expresses MTFR1L-V5 in mammalian cells

Depositor

Anne Brunet

Article

Schaffer et al Cell Metab. 2015 Nov 3;22(5):907-21. doi

Insert

Mitochondrial fission regulator 1-like (MTFR1L Human)

Use

Retroviral

Tags

v5

Expression

Mammalian

Mutation

Promoter

cmv

Available since

April 25, 2016

Availability

Academic Institutions and Nonprofits only

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FUW TetO HA-AMPKa1 WT

Plasmid

#69824

Purpose

Expresses AMPK alpha 1 in mammalian cells in a doxycycline inducible manner

Depositor

Anne Brunet

Article

Schaffer et al Cell Metab. 2015 Nov 3;22(5):907-21. doi

Insert

5'-AMP-activated protein kinase catalytic subunit alpha-1 (PRKAA1 Human)

Use

Lentiviral

Tags

HA

Expression

Mammalian

Mutation

Promoter

minimal CMV promoter with tetracycline operator

Available since

April 25, 2016

Availability

Academic Institutions and Nonprofits only

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pcDNA3-eGFP-NLS-RNF168

Plasmid

#133977

Purpose

mammalian expression vector of eGFP tagged wild type RNF168

Depositor

Daniel Durocher

Article

Stewart et al Cell. 2009 Feb 6;136(3):420-34. doi: 10.

Insert

RNF168 (RNF168 Human)

Use

Tags

eGFP

Expression

Mammalian

Mutation

siRNA resistant sequence: GAGGAGTCGTGTTTATTGA

Promoter

CMV

Available since

Nov. 4, 2019

Availability

Industry, Academic Institutions, and Nonprofits

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tetO-Hand2

Plasmid

#46028

Depositor

John Gearhart

Article

Addis et al J Mol Cell Cardiol. 2013 Apr 13;60C:97-1

Insert

Hand2 (Hand2 Mouse)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

tetO

Available since

July 22, 2013

Availability

Academic Institutions and Nonprofits only

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pLVH-EF1a-GFP-P2A-KLF4

Plasmid

#130694

Purpose

Lentiviral plasmids encoding human KLF4 with co-expressoin of GFP mediated by P2A.

Depositor

Kejin Hu

Article

Shao et al Nat Commun. 2016 Mar 7;7:10869. doi: 10.

Insert

human KLF4 (KLF4 Human)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Aug. 22, 2019

Availability

Academic Institutions and Nonprofits only

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GAL-hAR-658-919

Plasmid

#89082

Purpose

mammalian expression of human androgen receptor ligand binding domain 658-919 amino acids linked to GAL4 DNA binding domain

Depositor

Elizabeth Wilson

Article

Askew et al J Biol Chem. 2007 Aug 31;282(35):25801-1

Insert

hAR-658-919 (AR Human)

Use

Tags

GAL4 DBD

Expression

Mammalian

Mutation

aa 658-919

Promoter

Available since

April 11, 2017

Availability

Academic Institutions and Nonprofits only

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pLPC-FLAG.Mm.DMAP1

Plasmid

#184650

Purpose

Retroviral expression of mouse DMAP1

Depositor

Agnel Sfeir

Article

Barry et al Genes Dev. 2022 Feb 24. pii: gad.349039.

Insert

Dmap1 (Dmap1 Mouse)

Use

Retroviral

Tags

FLAG

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 24, 2022

Availability

Academic Institutions and Nonprofits only

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pET-15b-hAR-663-919

Plasmid

#89083

Purpose

bacterial expression of human androgen receptor ligand bindng domain amino acid residues 663-919 with histidine tag

Depositor

Elizabeth Wilson

Article

Askew et al J Biol Chem. 2007 Aug 31;282(35):25801-1

Insert

hAR ligand binding domain (AR Human)

Use

Tags

His

Expression

Bacterial

Mutation

aa 663-919

Promoter

T7

Available since

April 11, 2017

Availability

Academic Institutions and Nonprofits only

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pCAP03-sp44/p21

Plasmid

#120232

Purpose

Carries bidirectional promoter cassette containing sp44 and p21 promoters and apramycin resistance gene flanked by two FRT sites that can be amplified with homology sequence for refactoring.

Depositor

Bradley Moore

Article

Bauman et al Cell Chem Biol. 2019 Feb 15. pii: S2451-

Insert

bidirectional promoter cassette containing sp44 and p21 promoters and apramycin resistance gene flanked by two FRT sites

Use

Synthetic Biology

Tags

Expression

Mutation

aac(3)IV (apramycin resistance gene)

Promoter

Available since

March 21, 2019

Availability

Academic Institutions and Nonprofits only

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FUW TetO HA-AMPKa2 WT

Plasmid

#69826

Purpose

Expresses AMPK alpha 1 in mammalian cells in a doxycycline inducible manner

Depositor

Anne Brunet

Article

Schaffer et al Cell Metab. 2015 Nov 3;22(5):907-21. doi

Insert

5'-AMP-activated protein kinase catalytic subunit alpha-2 (PRKAA2 Human)

Use

Lentiviral

Tags

HA

Expression

Mammalian

Mutation

Promoter

minimal CMV promoter with tetracycline operator

Available since

May 11, 2016

Availability

Academic Institutions and Nonprofits only

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FUW TetO HA-AMPKa2 AS

Plasmid

#69827

Purpose

Expresses AMPK alpha 1 in mammalian cells in a doxycycline inducible manner

Depositor

Anne Brunet

Article

Schaffer et al Cell Metab. 2015 Nov 3;22(5):907-21. doi

Insert

5'-AMP-activated protein kinase catalytic subunit alpha-2 (PRKAA2 Human)

Use

Lentiviral

Tags

HA

Expression

Mammalian

Mutation

Promoter

minimal CMV promoter with tetracycline operator

Available since

May 12, 2016

Availability

Academic Institutions and Nonprofits only

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pcDNA4-His-Max-A-PQBP1

Plasmid

#25802

Depositor

Marius Sudol

Article

Tapia et al J Biol Chem. 2010 Apr 21. ():.

Insert

PQBP1 (PQBP1 Human)

Use

Tags

His and Xpress

Expression

Mammalian

Mutation

Promoter

Available since

July 21, 2010

Availability

Academic Institutions and Nonprofits only
pcFLAG-CSK-delta SH3 (73 to450 amino acids)

Plasmid

#74504

Purpose

Overexpression of CSK delta SH3 in mammalian cells

Depositor

Lars Rönnstrand

Article

Kazi et al Cell Signal. 2013 Sep;25(9):1852-60. doi

Insert

CSK (CSK Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

del-SH3 (73 to450 amino acids)

Promoter

CMV

Available since

May 2, 2016

Availability

Academic Institutions and Nonprofits only
pRK5 Myc-tagged PSK

Plasmid

#197149

Purpose

Expression of MYC-tagged PSK1-alpha / TAOK2 in mammalian cells

Depositor

Jonathan Morris

Article

Moore et al J Biol Chem. 2000 Feb 11;275(6):4311-22.

Insert

TAOK2 (TAOK2 Human)

Use

Tags

Myc

Expression

Mammalian

Mutation

Wild Type

Promoter

SV40

Available since

March 24, 2023

Availability

Academic Institutions and Nonprofits only

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FUW TetO HA-AMPKa1 AS

Plasmid

#69825

Purpose

Expresses AMPK alpha 1 in mammalian cells in a doxycycline inducible manner

Depositor

Anne Brunet

Article

Schaffer et al Cell Metab. 2015 Nov 3;22(5):907-21. doi

Insert

5'-AMP-activated protein kinase catalytic subunit alpha-1 (PRKAA1 Human)

Use

Lentiviral

Tags

HA

Expression

Mammalian

Mutation

Promoter

minimal CMV promoter with tetracycline operator

Available since

May 12, 2016

Availability

Academic Institutions and Nonprofits only

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pCS2-zfIl34

Plasmid

#168112

Purpose

Express zebrafish Il34, mRNA synthesis

Depositor

Petr Bartunek

Article

Hason et al Blood Adv. 2022 Jan 3. pii: 483305. doi:

Insert

Il34 (il34 Zebrafish)

Use

Vertebrate expression (zebrafish, chicken, xenopu…

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 15, 2022

Availability

Academic Institutions and Nonprofits only

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FUW TetO NET1A-V5 WT

Plasmid

#69828

Purpose

Expresses NET1A-V5 in mammalian cells in a doxycycline inducible manner

Depositor

Anne Brunet

Article

Schaffer et al Cell Metab. 2015 Nov 3;22(5):907-21. doi

Insert

Neuroepithelial cell-transforming gene 1 protein A (NET1 Human)

Use

Lentiviral

Tags

v5

Expression

Mammalian

Mutation

Promoter

minimal CMV promoter with tetracycline operator

Available since

April 25, 2016

Availability

Academic Institutions and Nonprofits only

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pECE M2-SH3PXD2A 8 serines mutated to alanines

Plasmid

#69816

Purpose

Expresses M2-SH3PXD2A in mammalian cells

Depositor

Anne Brunet

Article

Schaffer et al Cell Metab. 2015 Nov 3;22(5):907-21. doi

Insert

SH3 and PX domain-containing protein 2A (SH3PXD2A Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

Promoter

SV40 early promoter

Available since

May 12, 2016

Availability

Academic Institutions and Nonprofits only

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pECE M2-SH3PXD2A 6 serines mutated to alanines

Plasmid

#69815

Purpose

Expresses M2-SH3PXD2A in mammalian cells

Depositor

Anne Brunet

Article

Schaffer et al Cell Metab. 2015 Nov 3;22(5):907-21. doi

Insert

SH3 and PX domain-containing protein 2A (SH3PXD2A Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

Promoter

SV40 early promoter

Available since

May 12, 2016

Availability

Academic Institutions and Nonprofits only

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