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Showing: 1 - 20 of 18993 results

  2. pNOC_hfnCas12a_CRATES

    Plasmid
    #176251
    Purpose
    pNOC episomal plasmid harboring the humanized fnCas12a gene sequence tagged with Nlux and type IIS restriction site for endonuclease BaeI at the crRNA region that facilitates the generation of mulitpl
    Depositor
    John van der Oost
    Article
    Naduthodi et al ACS Synth Biol. 2021 Dec 17;10(12):3369-
    Insert
    humanized fnCas12a
    Use
    CRISPR, Luciferase, and Synthetic Biology ; Expre…
    Tags
    luciferase
    Expression
    Mutation
    Promoter
    Available since
    Nov. 16, 2021
    Availability
    Academic Institutions and Nonprofits only
    176251-plasmid-map-sequence-id-347017

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  4. pETSUMO2_Bacteroidetes-bGSDM

    Plasmid
    #212124
    Purpose
    For expression of the bacterial gasdermin (bGSDM) encoded by a Bacteroidetes (metagenomic) species. Protein is expressed with an N-terminal SUMO2 tag.
    Depositor
    Philip Kranzusch
    Article
    Johnson et al Science. 2022 Jan 14;375(6577):221-225.
    Insert
    Bacteroidetes bGSDM
    Use
    Tags
    6xHis-SUMO2
    Expression
    Bacterial
    Mutation
    N-terminal methionine deleted and replaced by sin…
    Promoter
    T7
    Available since
    Jan. 12, 2024
    Availability
    Academic Institutions and Nonprofits only
    212124-plasmid-map-sequence-id-419109

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  5. mEmerald-TES-C-14

    Plasmid
    #54276
    Purpose
    Localization: Focal Adhesions, Excitation: 487, Emission: 509
    Depositor
    Michael Davidson
    Insert
    TES (TES Human)
    Use
    Tags
    mEmerald
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    June 16, 2014
    Availability
    Industry, Academic Institutions, and Nonprofits

  6. pCS2+-HypocratesCS

    Plasmid
    #161960
    Purpose
    Mammalian expression of non-targeted inactivated control version of genetically encoded biosensor for (pseudo)hypohalous acids and their derivatives
    Depositor
    Dmitry Bilan
    Article
    Kostyuk et al Nat Commun. 2022 Jan 10;13(1):171. doi:
    Insert
    Hypocrates
    Use
    Tags
    Expression
    Mammalian
    Mutation
    changed Cysteine 355 to Serine
    Promoter
    CMV, SP6
    Available since
    Feb. 10, 2021
    Availability
    Academic Institutions and Nonprofits only
    161960-plasmid-map-sequence-id-324303

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  9. mEmerald-TES-N-14

    Plasmid
    #54277
    Purpose
    Localization: Focal Adhesions, Excitation: 487, Emission: 509
    Depositor
    Michael Davidson
    Insert
    TES (TES Human)
    Use
    Tags
    mEmerald
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    June 16, 2014
    Availability
    Industry, Academic Institutions, and Nonprofits

  10. mEos3.2-TES-N-14

    Plasmid
    #57482
    Purpose
    Localization: Focal Adhesions, Excitation: 507 / 572, Emission: 516 / 580
    Depositor
    Michael Davidson
    Insert
    TES (TES Human)
    Use
    Tags
    mEos3.2
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Feb. 27, 2015
    Availability
    Industry, Academic Institutions, and Nonprofits
    57482-plasmid-map-sequence-id-213111

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  11. mTagBFP2-TES-N-14

    Plasmid
    #62786
    Purpose
    Localization: Focal Adhesions, N1 Cloning Vector, Excitation:399, Emission:456
    Depositor
    Michael Davidson
    Insert
    TES (TES Human)
    Use
    Tags
    mTagBFP2
    Expression
    Mammalian
    Mutation
    none
    Promoter
    CMV
    Available since
    March 10, 2015
    Availability
    Industry, Academic Institutions, and Nonprofits
    62786-plasmid-map-sequence-id-173171

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  12. pQE30-HypocratesCS

    Plasmid
    #161957
    Purpose
    Bacterial expression of inactivated control version of genetically encoded biosensor for (pseudo)hypohalous acids and their derivatives
    Depositor
    Dmitry Bilan
    Article
    Kostyuk et al Nat Commun. 2022 Jan 10;13(1):171. doi:
    Insert
    HypocratesCS
    Use
    Tags
    6xHIs tag
    Expression
    Bacterial
    Mutation
    changed cysteine 355 to serine
    Promoter
    T5
    Available since
    May 4, 2021
    Availability
    Academic Institutions and Nonprofits only
    161957-plasmid-map-sequence-id-324311

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  13. mEos2-TES-N-14

    Plasmid
    #57428
    Purpose
    Localization: Focal Adhesions, Excitation: 505 / 569, Emission: 516 / 581
    Depositor
    Michael Davidson
    Insert
    TES (TES Human)
    Use
    Tags
    mEos2
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Feb. 5, 2015
    Availability
    Industry, Academic Institutions, and Nonprofits

  16. pETSUMO2_Bacteroidetes-bGSDM-TEV

    Plasmid
    #222101
    Purpose
    For expression of a mutant bacterial gasdermin (bGSDM) encoded by a Bacteroidetes species with a TEV protease cleavage site for controlled activation.
    Depositor
    Philip Kranzusch
    Article
    Johnson et al Nature. 2024 Apr;628(8008):657-663. doi:
    Insert
    Bacteroidetes-bGSDM-TEV ORF
    Use
    Tags
    6xHis-SUMO2
    Expression
    Bacterial
    Mutation
    TEV protease site replaces residues E242–G248
    Promoter
    T7
    Available since
    July 12, 2024
    Availability
    Academic Institutions and Nonprofits only
    222101-plasmid-map-sequence-id-440273

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Showing: 1 - 20 of 18993 results