GAL-hAR-658-919
(Plasmid
#89082)
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Purposemammalian expression of human androgen receptor ligand binding domain 658-919 amino acids linked to GAL4 DNA binding domain
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 89082 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepGALO
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberLow Copy
Gene/Insert
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Gene/Insert namehAR-658-919
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SpeciesH. sapiens (human)
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Mutationaa 658-919
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Entrez GeneAR (a.k.a. AIS, AR8, DHTR, HUMARA, HYSP1, KD, NR3C4, SBMA, SMAX1, TFM)
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Tag
/ Fusion Protein
- GAL4 DBD (N terminal on backbone)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site TthIII (unknown if destroyed)
- 3′ cloning site Csp45I (unknown if destroyed)
Resource Information
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Article Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
This vector contains a short VP16 activation domain (AA 411-456.)
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
GAL-hAR-658-919 was a gift from Elizabeth Wilson (Addgene plasmid # 89082 ; http://n2t.net/addgene:89082 ; RRID:Addgene_89082) -
For your References section:
Modulation of androgen receptor activation function 2 by testosterone and dihydrotestosterone. Askew EB, Gampe RT Jr, Stanley TB, Faggart JL, Wilson EM. J Biol Chem. 2007 Aug 31;282(35):25801-16. doi: 10.1074/jbc.M703268200. Epub 2007 Jun 25. 10.1074/jbc.M703268200 PubMed 17591767