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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 46028 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonetetO-Gateway
- Total vector size (bp) 10000
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Modifications to backboneHand2 cloned into Gateway site of tetO-Gateway
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Vector typeMammalian Expression, Lentiviral
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Selectable markersZeo marker is outside the LTRs and will not be packaged into virus.
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)Stbl3
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameHand2
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SpeciesM. musculus (mouse)
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Insert Size (bp)715
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Entrez GeneHand2 (a.k.a. AI225906, AI661148, Ehan, Ehand2, Hed, Th2, Thin, Thing2, bHLHa, bHLHa26, dHA, dHAND)
- Promoter tetO
Cloning Information
- Cloning method Gateway Cloning
- 5′ sequencing primer T7 (Common Sequencing Primers)
Resource Information
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Article Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
tetO-Hand2 was a gift from John Gearhart (Addgene plasmid # 46028 ; http://n2t.net/addgene:46028 ; RRID:Addgene_46028) -
For your References section:
Optimization of direct fibroblast reprogramming to cardiomyocytes using calcium activity as a functional measure of success. Addis RC, Ifkovits JL, Pinto F, Kellam LD, Esteso P, Rentschler S, Christoforou N, Epstein JA, Gearhart JD. J Mol Cell Cardiol. 2013 Apr 13;60C:97-106. doi: 10.1016/j.yjmcc.2013.04.004. 10.1016/j.yjmcc.2013.04.004 PubMed 23591016