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Plasmid#15503DepositorInsertPRDM16 (Prdm16 Mouse)
UseTagsExpressionMammalianMutationPromoterAvailable sinceDec. 14, 2007AvailabilityAcademic Institutions and Nonprofits only -
pGWB533
Plasmid#74872PurposeGateway cloning compatible binary vector for C-terminal fusion with GUS (no promoter).DepositorTypeEmpty backboneUseTagsExpressionPlantMutationPromoterAvailable sinceOct. 18, 2016AvailabilityAcademic Institutions and Nonprofits only -
pDRM166 LKB (Luciferase mKate2 Blast)
Plasmid#183502PurposeExpresses the firefly luciferase gene, the NLS-tagged mKate2 gene, and the blasticidin resistance gene joined by P2A linkers.DepositorInsertsFirefly Luciferase
mKate2
BSD
UseLentiviral and LuciferaseTagsP2A linker and SV40 NLS with GGS linkerExpressionMammalianMutationPromoterMND, MND (off Luc-P2A), and MND (off Luc-P2A-mKat…Available sinceNov. 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCIpA102-G-HLA-A2_GFP
Plasmid#85162Purposefor mRNA expression of HLA.A2-EGFPDepositorInsertHLA.A2 (HLA-A Human)
UseMrna expressionTagsEGFPExpressionMammalianMutationPromoterCMVAvailable sinceJan. 4, 2017AvailabilityAcademic Institutions and Nonprofits only -
pEGFP hsp70
Plasmid#15215DepositorInserthsp70 (HSPA1A Human)
UseTagsEGFPExpressionMammalianMutationPromoterAvailable sinceJune 15, 2007AvailabilityAcademic Institutions and Nonprofits only -
BLBP-GFP
Plasmid#63174PurposeExpressing GFP from BLBP promoter which is a neural stem cell (radial glial cells) specific promoterDepositorInsertBLBP (Fabp7 Mouse)
UseTagsExpressionMammalianMutationPromoter1700bp upstream of BLBP geneAvailable sinceJuly 7, 2015AvailabilityAcademic Institutions and Nonprofits only -
pX330-PITCh-TUBA1B
Plasmid#207763PurposeExpresses SpCas9, the PITCh gRNA, and a sgRNA targeting the N-terminus of TUBA1B for knock-in.DepositorArticleInsertsgRNA Targeting N-terminus of TUBA1B (TUBA1B Human)
UseCRISPRTagsExpressionMammalianMutationPromoterU6Available sinceNov. 29, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET-19b_MMLV-RT
Plasmid#153312PurposeBacterial expression of MMLV reverse transcriptaseDepositorInsertMMLV Reverse Transcriptase
UseTags10x HisExpressionBacterialMutationPromoterAvailable sinceJune 2, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pBABEpuro mTurquoise2 LC3B
Plasmid#78518PurposeFluorescent reporter for LC3B visualizationDepositorInsertLC3B (MAP1LC3B Human)
UseRetroviralTagsmTurquoise2ExpressionMammalianMutationPromoterretroviralAvailable sinceJan. 5, 2017AvailabilityAcademic Institutions and Nonprofits only -
GSK3 beta pGEX
Plasmid#15898DepositorInsertGSK3 beta (GSK3B Human)
UseTagsGSTExpressionBacterialMutationPromoterAvailable sinceNov. 1, 2007AvailabilityIndustry, Academic Institutions, and Nonprofits -
mCherry-MAPTau-C-10
Plasmid#55077PurposeLocalization: Microtubules, Excitation: 587, Emission: 610DepositorInsertMAPTau (MAPT Human)
UseTagsmCherryExpressionMammalianMutationPromoterCMVAvailable sinceAug. 29, 2014AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-Myc-14-3-3ζ
Plasmid#48798Purposeexpresses human myc-14-3-3ζ in mammalian cellsDepositorInsertYWHAZ (YWHAZ Human)
UseTagsmycExpressionMammalianMutationPromoterCMVAvailable sinceMay 20, 2014AvailabilityAcademic Institutions and Nonprofits only -
peYFP-C1-mTOR
Plasmid#73384PurposeExpresses YFP tagged mTORDepositorInsertmTOR (MTOR Human)
UseTagsYFPExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 24, 2016AvailabilityAcademic Institutions and Nonprofits only -
pLX304_CMV::mCherry-LgBiT-FLAG-NES
Plasmid#199715PurposeExpresses LgBiT, fused to mCherry, in the cytosol of mammalian cells. Can be used in generating stable cell lines via lentiviral transduction.DepositorInsertmCherry-LgBiT-FLAG-NES
UseLentiviral and LuciferaseTagsExpressionMammalianMutationPromoterCMVAvailable sinceMay 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
pTRIP-SFFV-Hygro-2A-TMRPSS2
Plasmid#170390PurposeGeneration of stable lines to enhance SARS-CoV-2 infectivity in ACE2 expressing cellsDepositorInsertTMPRSS2 (TMPRSS2 Human)
UseLentiviralTagsExpressionMutationPromoterSFFVAvailable sinceJune 23, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
pD649-HAsp-SIGLEC5-Fc(DAPA)-AviTag-6xHis
Plasmid#156853PurposeMammalian expression of cell-surface protein extracellular domain fused to Fc(DAPA)-Avi-6xHis. Protein is secreted from cells.DepositorInsertSIGLEC5 (SIGLEC5 Human)
UseTagsFc(DAPA)-AviTag-6xHisExpressionMammalianMutationFc region of human IgG contains D265A and P329A m…PromoterCMV/SP6Available sinceMarch 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
TFORF1974
Plasmid#143988PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertTBX3 (TBX3 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceFeb. 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
qTAG-N-Solo-mScarlet-TUBA1B
Plasmid#207764PurposeDonor template for mScarlet insertion into the N-terminus of the TUBA1B locus for tubulin visualization. To be co-transfected with sgRNA plasmid px330-PITCh-TUBA1B Addgene #207763DepositorArticleInsertTUBA1B Homology Arms flanking a mScarlet Tag (TUBA1B Human)
UseCRISPR; Donor templateTagsExpressionMammalianMutationPromoterAvailable sinceNov. 15, 2023AvailabilityIndustry, Academic Institutions, and Nonprofits -
pAHYB-GFP
Plasmid#85074PurposeExpression of GFP in Pichia pastorisDepositorInsertGFP
UseTagsExpressionYeastMutationPromoterAOX1Available sinceMarch 17, 2017AvailabilityAcademic Institutions and Nonprofits only -
tetO-NR2F1
Plasmid#170698Purposedoxycycline-inducible overexpression of NR2F1DepositorInsertnuclear receptor subfamily 2 group F member 1 (NR2F1 Human)
UseLentiviral; Doxycycline inducibleTagsExpressionMammalianMutationdeletion of 36G- please see depositor commentsPromoterTRE promoter, Tet-ONAvailable sinceJuly 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLenti6-H2B-mCherry
Plasmid#89766PurposeLentivirus destination vector for stable expression of mCherry-tagged histone H2BDepositorInsertHistone H2B (H2BC11 Human)
UseLentiviralTagsmCherryExpressionMammalianMutationPromoterCMVAvailable sinceSept. 23, 2017AvailabilityAcademic Institutions and Nonprofits only -
Inducible Caspex expression
Plasmid#97421PurposeFor stable clone generation of cells expressing the dCas9-APEX2 fusion protein for Genomic locus proteomicsDepositorInsertsCaspex
T2A-Gfp
UseCRISPRTags3xFLAG and V5ExpressionMammalianMutationdCas9: D10A and H840A, APEX2: K14D, W41F, E112K, …PromoterTRE3GAvailable sinceOct. 23, 2017AvailabilityAcademic Institutions and Nonprofits only -
pJG/ALPHA MHC
Plasmid#55594Purposetransgene expression driven by ALPHA MHC promoterDepositorTypeEmpty backboneUseTagsExpressionMammalianMutationPromoteralpha MHCAvailable sinceJuly 2, 2014AvailabilityAcademic Institutions and Nonprofits only -
9ao-SOD2-3xHA-TALE(ND4-Left)-Nt.BspD6I(C)
Plasmid#209293PurposemitoBE expression vector, target mtND4 gene.DepositorInsertSOD2-3xHA-TALE(ND4-Left)-Nt.BspD6I(C)
UseTagsExpressionMammalianMutationPromoterAvailable sinceNov. 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pDisplay-GRAB_r5-HT1.0
Plasmid#208713PurposeExpresses the red 5-HT sensor GRAB_r5-HT1.0 in mammalian cellsDepositorInsertRed fluorescent 5-HT sensor GRAB_r5-HT1.0
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceOct. 25, 2023AvailabilityAcademic Institutions and Nonprofits only -
ppyCAG_RNaseH1_D210N
Plasmid#111904PurposeExpress D210N mutated RNASEH1 which abolishes the catalytic activity of RNASEH1DepositorInsertRNASEH1 (RNASEH1 Human)
UseTagsV5ExpressionMammalianMutationChanged D 210 to NPromoterAvailable sinceJuly 4, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCDH-EF1-Luc2-cG-BSD
Plasmid#124982PurposeExpress Luc2, copGFPDepositorInsertLuc2, copGFP
UseLentiviralTagsExpressionMutationPromoterEF1-alphaAvailable sinceMay 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAV-U6+27-Tornado-Broccoli
Plasmid#124360PurposeOverexpression of circular RNA (Broccoli)DepositorInsertTornado-Broccoli
UseTagsExpressionMammalianMutationPromoterU6+27Available sinceMay 3, 2019AvailabilityAcademic Institutions and Nonprofits only -
GFP-Drosha
Plasmid#62520Purposewild-type human Drosha cDNA is subcloned into pEGFP-C1 vector between HindIII and KpnI sitesDepositorInserthuman Drosha full length (DROSHA Human)
UseTagsGFPExpressionMammalianMutationPromoterAvailable sinceMarch 20, 2015AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-N1-ACTR3
Plasmid#8462DepositorInsertACTR3 (ACTR3 Human)
UseTagsGFPExpressionMammalianMutationPromoterAvailable sinceAug. 7, 2006AvailabilityAcademic Institutions and Nonprofits only -
19ag-SOD2-3xHA-TALE(RNR2-Left)-MutH
Plasmid#209294PurposemitoBE expression vector, target mtRNR2 gene.DepositorInsertSOD2-3xHA-TALE(RNR2-Left)-MutH
UseTagsExpressionMammalianMutationPromoterAvailable sinceNov. 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
mCherry-LaminB1-10
Plasmid#55069PurposeLocalization: Nuclear Envelope, Excitation: 587, Emission: 610DepositorInsertLaminB1 (LMNB1 Human)
UseTagsmCherryExpressionMammalianMutationPromoterCMVAvailable sinceOct. 7, 2014AvailabilityAcademic Institutions and Nonprofits only -
pLV-EF1a-IRES-Puro
Plasmid#85132Purposelentiviral backboneDepositorHas ServiceCloning Grade DNATypeEmpty backboneUseLentiviralTagsExpressionMutationPromoterEF1aAvailable sinceFeb. 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hsyn-NE2h
Plasmid#208687PurposeExpresses the genetically-encoded fluorescent norepinephrine (NE) sensor GRAB_NE2h in neuronsDepositorInsertGPCR activation based norepinephrine (NE) sensor GRAB_NE2h
UseAAVTagsExpressionMutationPromoterhSynAvailable sinceOct. 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pSV human p85 alpha (HA tag)
Plasmid#11499DepositorInsertp85 alpha (PIK3R1 Human)
UseTagsHAExpressionMammalianMutationPromoterAvailable sinceJuly 19, 2006AvailabilityAcademic Institutions and Nonprofits only -
pIRESneo-FLAG/HA Ago2 corrected
Plasmid#10822DepositorInsertArgonaute 2 (AGO2 Human)
UseTagsFLAG and HAExpressionMammalianMutation3 silent mutations in orfPromoterAvailable sinceNov. 2, 2005AvailabilityAcademic Institutions and Nonprofits only -
GFP-C1(2)delta
Plasmid#21216DepositorInsertGFP-N2-PKCdelta-C1 (Prkcd Rat)
UseTagsGFPExpressionMammalianMutationC1(2) domains of PKC delta (rat) (Lab plasmid WC…PromoterAvailable sinceJuly 10, 2009AvailabilityAcademic Institutions and Nonprofits only -
pLenti6.3/EV
Plasmid#120848PurposeControl vector, 20nt random sequence between gateway recombination sitesDepositorTypeEmpty backboneUseLentiviralTagsExpressionMutationPromoterAvailable sinceFeb. 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
pUAS-V5-TurboID-NES
Plasmid#116904PurposeExpresses TurboID under Gal4/UAS control. TurboID is tagged at the N-terminus with V5 and a nuclear export signal (NES) at the C-terminus.DepositorInsertTurboID
UseDrosophila expressionTagsExpressionMutationPromoterAvailable sinceOct. 3, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCAGGS-PKCdelta-EGFP
Plasmid#178856PurposeMammalian expression of PKCδ fused to EGFPDepositorInsertPKCδ (PRKCD Human)
UseTagsEGFPExpressionMammalianMutationPromoterCAGAvailable sinceJuly 14, 2022AvailabilityAcademic Institutions and Nonprofits only