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Addgene

pLX304_CMV::mCherry-LgBiT-FLAG-NES
(Plasmid #199715)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 199715 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pLX304
  • Vector type
    Mammalian Expression, Lentiviral, Luciferase
  • Selectable markers
    Blasticidin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    mCherry-LgBiT-FLAG-NES
  • Species
    Synthetic
  • Promoter CMV

Cloning Information

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please visit https://doi.org/10.1101/2023.03.03.531050 for bioRxiv preprint. In the original study XL1 Blue E. coli are used for amplifying plasmids.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pLX304_CMV::mCherry-LgBiT-FLAG-NES was a gift from Alice Ting (Addgene plasmid # 199715 ; http://n2t.net/addgene:199715 ; RRID:Addgene_199715)
  • For your References section:

    Cell-Type-Specific Intracellular Protein Delivery with Inactivated Botulinum Neurotoxin. Roh H, Dorner BG, Ting AY. J Am Chem Soc. 2023 May 10;145(18):10220-10226. doi: 10.1021/jacs.3c01145. Epub 2023 Apr 26. 10.1021/jacs.3c01145 PubMed 37098770