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Addgene

GSK3 beta pGEX
(Plasmid #15898)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 15898 Standard format: Plasmid sent in bacteria as agar stab 1 $85 *

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Backbone

  • Vector backbone
    pGEX
  • Backbone size w/o insert (bp) 5000
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    GSK3 beta
  • Alt name
    GSK3b
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1400
  • Entrez Gene
    GSK3B
  • Tag / Fusion Protein
    • GST (N terminal on backbone)

Cloning Information

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

ORF is at 258-2237.

The Addgene Quality Control sequencing results have found silent nucleotide changes in the depositor GSK3 beta sequence as well as an amino acid change. The verified sequence matches PBD Sequence ID: 4IQ6_A and encodes an isoform of human GSK3 beta that should function as WT, please review the Addgene QC sequence before use.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    GSK3 beta pGEX was a gift from Jim Woodgett (Addgene plasmid # 15898 ; http://n2t.net/addgene:15898 ; RRID:Addgene_15898)