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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 15898 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 * |
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Backbone
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Vector backbonepGEX
- Backbone size w/o insert (bp) 5000
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameGSK3 beta
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Alt nameGSK3b
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SpeciesH. sapiens (human)
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Insert Size (bp)1400
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Entrez GeneGSK3B
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Tag
/ Fusion Protein
- GST (N terminal on backbone)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ sequencing primer pGEX5'
- 3′ sequencing primer pGEX3' (Common Sequencing Primers)
Resource Information
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Article Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
ORF is at 258-2237.
The Addgene Quality Control sequencing results have found silent nucleotide changes in the depositor GSK3 beta sequence as well as an amino acid change. The verified sequence matches PBD Sequence ID: 4IQ6_A and encodes an isoform of human GSK3 beta that should function as WT, please review the Addgene QC sequence before use.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
GSK3 beta pGEX was a gift from Jim Woodgett (Addgene plasmid # 15898 ; http://n2t.net/addgene:15898 ; RRID:Addgene_15898)