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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 14755 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 * |
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Backbone
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Vector backbonepcDNA3
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Backbone manufacturerInvitrogen
- Backbone size w/o insert (bp) 5500
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Vector typeMammalian Expression
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameGSK3 beta K85A
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Alt nameGSK3b
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SpeciesH. sapiens (human)
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Insert Size (bp)1300
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MutationK85A. Kinase dead mutant.
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Entrez GeneGSK3B
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Tag
/ Fusion Protein
- HA (C terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ sequencing primer T7 (Common Sequencing Primers)
Resource Information
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Articles Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Nru I site is added to the K->A mutation
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
HA GSK3 beta K85A pcDNA3 was a gift from Jim Woodgett (Addgene plasmid # 14755 ; http://n2t.net/addgene:14755 ; RRID:Addgene_14755) -
For your References section:
Modulation of the glycogen synthase kinase-3 family by tyrosine phosphorylation. Hughes K, Nikolakaki E, Plyte SE, Totty NF, Woodgett JR. EMBO J. 1993 Feb . 12(2):803-8. PubMed 8382613