Skip to main content
Addgene

HA GSK3 beta wt pcDNA3
(Plasmid #14753)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 14753 Standard format: Plasmid sent in bacteria as agar stab 1 $85 *

* Log in to view industry pricing.

Backbone

  • Vector backbone
    pcDNA3
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 5500
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    GSK3 beta
  • Alt name
    GSK3b
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1300
  • Entrez Gene
    GSK3B
  • Tag / Fusion Protein
    • HA (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site XbaI (not destroyed)
  • 5′ sequencing primer T7
  • (Common Sequencing Primers)

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    HA GSK3 beta wt pcDNA3 was a gift from Jim Woodgett (Addgene plasmid # 14753 ; http://n2t.net/addgene:14753 ; RRID:Addgene_14753)
  • For your References section:

    Glycogen synthase kinase-3 and dorsoventral patterning in Xenopus embryos. He X, Saint-Jeannet JP, Woodgett JR, Varmus HE, Dawid IB. Nature. 1995 Apr 13. 374(6523):617-22. 10.1038/374617a0 PubMed 7715701