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PurposeMammalian expression of human GSK3 beta with HA tag
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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 14753 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 * |
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Backbone
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Vector backbonepcDNA3
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Backbone manufacturerInvitrogen
- Backbone size w/o insert (bp) 5500
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Vector typeMammalian Expression
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameGSK3 beta
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Alt nameGSK3b
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SpeciesH. sapiens (human)
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Insert Size (bp)1300
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Entrez GeneGSK3B
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Tag
/ Fusion Protein
- HA (C terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site EcoRI (not destroyed)
- 3′ cloning site XbaI (not destroyed)
- 5′ sequencing primer T7 (Common Sequencing Primers)
Resource Information
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Articles Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
HA GSK3 beta wt pcDNA3 was a gift from Jim Woodgett (Addgene plasmid # 14753 ; http://n2t.net/addgene:14753 ; RRID:Addgene_14753) -
For your References section:
Glycogen synthase kinase-3 and dorsoventral patterning in Xenopus embryos. He X, Saint-Jeannet JP, Woodgett JR, Varmus HE, Dawid IB. Nature. 1995 Apr 13. 374(6523):617-22. 10.1038/374617a0 PubMed 7715701