tetO-NR2F1
(Plasmid
#170698)
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Purposedoxycycline-inducible overexpression of NR2F1
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 170698 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backboneFU-tetO-Gateway
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Backbone manufactureraddgene plasmid #43914
- Backbone size w/o insert (bp) 8600
- Total vector size (bp) 9879
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Modifications to backboneNR2F1 ORF added to plasmid using Gateway cloning
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Vector typeMammalian Expression, Lentiviral ; Doxycycline Inducible
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature30°C
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Growth Strain(s)NEB Stable
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Copy numberLow Copy
Gene/Insert
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Gene/Insert namenuclear receptor subfamily 2 group F member 1
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Alt nameNR2F1
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Alt nameBBOAS | BBSOAS | COUP-TFI | EAR-3 | EAR3 | ERBAL3 | NR2F2 | SVP44 | TCFCOUP1 | TFCOUP1
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SpeciesH. sapiens (human)
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Mutationdeletion of 36G- please see depositor comments
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GenBank ID3912075
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Entrez GeneNR2F1 (a.k.a. BBOAS, BBSOAS, COUP-TFI, COUPTF1, EAR-3, EAR3, ERBAL3, SVP44, TCFCOUP1, TFCOUP1)
- Promoter TRE promoter, Tet-ON
Cloning Information
- Cloning method Gateway Cloning
- 5′ sequencing primer LNCX; T7 (Common Sequencing Primers)
Resource Information
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A portion of this plasmid was derived from a plasmid made byNR2F1 cDNA was obtained from Dharmacon (MHS6278-202757063)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Please note 36G is deleted from the insert. Depositor confirms this does not effect plasmid function.
This plasmid was generated using Gateway cloning. attB sites were added to NR2F1 via PCR reaction using pCMV-SPORT6-NR2F1 as a template. Resulting fragements were recombined with pDONR221 to generate an entry clone. LR reaction was performed using the destination vector FU-tetO-Gateway (Addgene Plasmid #19778).
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
tetO-NR2F1 was a gift from Paul Burridge (Addgene plasmid # 170698 ; http://n2t.net/addgene:170698 ; RRID:Addgene_170698) -
For your References section:
A novel transcription factor combination for direct reprogramming to a spontaneously contracting human cardiomyocyte-like state. Romero-Tejeda M, Fonoudi H, Weddle CJ, DeKeyser JM, Lenny B, Fetterman KA, Magdy T, Sapkota Y, Epting C, Burridge PW. J Mol Cell Cardiol. 2023 Jul 6:S0022-2828(23)00106-2. doi: 10.1016/j.yjmcc.2023.06.005. 10.1016/j.yjmcc.2023.06.005 PubMed 37421991