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Plasmid#196861PurposeExpression of neural precursor cell expressed developmentally down-regulated 1 (Nedd1) fused to mOrange2 and rat (r) Centrosomin motif 1 (CM1). Nedd1 targets CM1 to the centrosome to activate gamma-TuRC.DepositorInsertNedd1-mOrange2-rCM1 (Nedd1 Rat)
UseTagsExpressionMammalianMutationPromoterChicken bactin (plus Chicken bactin intron)Available sinceMay 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBactin-Nedd1-mOrange2-F75A
Plasmid#196862PurposeExpression of Need1-rCM1 harboring a (F75A) mutation in the rat (r) Centrosomin motif 1 (CM1) and fused to mOrange2. F75A mutatuon renders CM1 incapable of binding and activating gamma-TuRCDepositorInsertNedd1-mOrange2-F75A (Nedd1 Rat)
UseTagsExpressionMammalianMutationF75A in the CM1 domainPromoterChicken bactin (plus Chicken bactin intron)Available sinceMay 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCI pHluorin Syt12
Plasmid#184513PurposeMammalian expression of Synaptotagmin 12 with pH-sensitive fluorescent proteinDepositorInsertSynaptotagmin 12 (Syt12 Rat)
UseTagsSuper Ecliptic pHluorinExpressionMammalianMutationPromoterAvailable sinceMay 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCI pHluorin Syt17
Plasmid#184514PurposeMammalian expression of Synaptotagmin 17 with pH-sensitive fluorescent proteinDepositorInsertSynaptotagmin 17 (Syt17 Rat)
UseTagsSuper Ecliptic pHluorinExpressionMammalianMutationPromoterAvailable sinceMay 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
FUGW-APOBEC1-YTH-mCherry
Plasmid#197035PurposeExpress APOBEC1 cytosine deaminase fused with YTH m6A binding domain from UbiC promoter.DepositorInsertAPOBEC1-YTH
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceMay 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
pEGFPC2-gephyrin C3
Plasmid#199610PurposeEncodes N-terminal eGFP-tagged gephyrin including the C3 splice cassette for expression in mammalian cells.DepositorInsertGephyrin (Gphn Rat)
UseTagsEGFPExpressionMammalianMutationC3 cassette insertionPromoterCMVAvailable sinceMay 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
pEGFPC2-gephyrin C4a
Plasmid#199611PurposeEncodes N-terminal eGFP-tagged gephyrin including the C4a splice cassette for expression in mammalian cells.DepositorInsertGephyrin (Gphn Rat)
UseTagsEGFPExpressionMammalianMutationC4a casette insertionPromoterCMVAvailable sinceMay 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGL3-MTS-ND6-W82-R15-G1333C-UGI-Puro
Plasmid#198861PurposeEmploy DdCBE-mediated mtDNA editing to ablate mtProtein(ND6) by swapping Trp codon TGA with stop codon TAA in C6 cellsDepositorInsertND6-W82-R15 TALEN
UseTagsExpressionMammalianMutationPromoterAvailable sinceMay 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGL3-MTS-ND5-W68-R15-G1333N-UGI-Puro
Plasmid#198859PurposeEmploy DdCBE-mediated mtDNA editing to ablate mtProtein(ND5) by swapping Trp codon TGA with stop codon TAA in C6 cellsDepositorInsertND5-W68-R15 TALEN
UseTagsExpressionMammalianMutationPromoterAvailable sinceMay 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGL3-MTS-ND4-W24-R17-G1333N-UGI-Puro
Plasmid#198857PurposeEmploy DdCBE-mediated mtDNA editing to ablate mtProtein(ND4) by swapping Trp codon TGA with stop codon TAA in C6 cellsDepositorInsertND4-W24-R17 TALEN
UseTagsExpressionMammalianMutationPromoterAvailable sinceMay 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGL3-MTS-ND3-Q26-R19-G1397N-UGI-Puro
Plasmid#198855PurposeEmploy DdCBE-mediated mtDNA editing to ablate mtProtein(ND3) by swapping Gln codon CAA with stop codon TAA in C6 cellsDepositorInsertND3-Q26-R19 TALEN
UseTagsExpressionMammalianMutationPromoterAvailable sinceMay 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGL3-MTS-ND2-W30-R18-G1333N-UGI-Puro
Plasmid#198853PurposeEmploy DdCBE-mediated mtDNA editing to ablate mtProtein(ND2) by swapping Trp codon TGA with stop codon TAA in C6 cellsDepositorInsertND2-W30-R18 TALEN
UseTagsExpressionMammalianMutationPromoterAvailable sinceMay 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGL3-MTS-CYTB-W77-R14-G1333N-UGI-Puro
Plasmid#198849PurposeEmploy DdCBE-mediated mtDNA editing to ablate mtProtein(CYTB) by swapping Trp codon TGA with stop codon TAA in C6 cellsDepositorInsertCYTB-W77-R14 TALEN
UseTagsExpressionMammalianMutationPromoterAvailable sinceMay 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGL3-MTS-COX3-W16-R15-G1333N-UGI-Puro
Plasmid#198847PurposeEmploy DdCBE-mediated mtDNA editing to ablate mtProtein(COX3) by swapping Trp codon TGA with stop codon TAA in C6 cellsDepositorInsertCOX3-W16-R15 TALEN
UseTagsExpressionMammalianMutationPromoterAvailable sinceMay 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGL3-MTS-COX2-Q6-R18-G1333C-UGI-Puro
Plasmid#198845PurposeEmploy DdCBE-mediated mtDNA editing to ablate mtProtein(COX2) by swapping Gln codon CAA with stop codon TAA in C6 cellsDepositorInsertCOX2-Q6-R18 TALEN
UseTagsExpressionMammalianMutationPromoterAvailable sinceMay 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGL3-MTS-COX1-W25-R18-G1333N-UGI-Puro
Plasmid#198843PurposeEmploy DdCBE-mediated mtDNA editing to ablate mtProtein(COX1) by swapping Trp codon TGA with stop codon TAA in C6 cellsDepositorInsertCOX1-W25-R18 TALEN
UseTagsExpressionMammalianMutationPromoterAvailable sinceMay 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGL3-MTS-ATP8-W9-R15-G1333N-UGI-Puro
Plasmid#198841PurposeEmploy DdCBE-mediated mtDNA editing to ablate mtProtein(ATP8) by swapping Trp codon TGA with stop codon TAA in C6 cellsDepositorInsertATP8-W9-R15 TALEN
UseTagsExpressionMammalianMutationPromoterAvailable sinceMay 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGL3-MTS-ATP6-W68-R17-G1333N-UGI-Puro
Plasmid#198839PurposeEmploy DdCBE-mediated mtDNA editing to ablate mtProtein(ATP6) by swapping Trp codon TGA with stop codon TAA in C6 cellsDepositorInsertATP6-W68-R17 TALEN
UseTagsExpressionMammalianMutationPromoterAvailable sinceMay 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLJM1-Lamp1 FLAG
Plasmid#199750PurposeLentiviral construct to stably express Lamp1DepositorInsertLamp1 (Lamp1 Rat)
UseTagsFlagExpressionMammalianMutationPromoterEF1aAvailable sinceMay 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCI pHluorin Syt3
Plasmid#184504PurposeMammalian expression of Synaptotagmin 3 with pH-sensitive fluorescent proteinDepositorInsertSynaptotagmin 3 (Syt3 Rat)
UseTagsSuper Ecliptic pHluorinExpressionMammalianMutationPromoterAvailable sinceMay 2, 2023AvailabilityAcademic Institutions and Nonprofits only