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A DARPin-based molecular toolset to probe gephyrin and inhibitory synapse biology.
Campbell BFN, Dittmann A, Dreier B, Pluckthun A, Tyagarajan SK
Elife. 2022 Oct 31;11:e80895. doi: 10.7554/eLife.80895. PubMed Article

Plasmids from Article

ID Plasmid Purpose
199610pEGFPC2-gephyrin C3Encodes N-terminal eGFP-tagged gephyrin including the C3 splice cassette for expression in mammalian cells.
199611pEGFPC2-gephyrin C4aEncodes N-terminal eGFP-tagged gephyrin including the C4a splice cassette for expression in mammalian cells.
199612pQIq_MRGS_HIS8_( E3_5)_FLAGEncodes DARPin-FLAG E3_5 for bacterial expression and column purification
199613pQIq_MRGS_HIS8_(27B3)_FLAGEncodes DARPin-FLAG 27B3 for bacterial expression and column purification
199615pQIq_MRGS_HIS8_(27F3)_FLAGEncodes DARPin-FLAG 27F3 for bacterial expression and column purification
199616pQIq_MRGS_HIS8_(27G2)_FLAGEncodes DARPin-FLAG 27G2 for bacterial expression and column purification
199617pcDNA3.1_ E3_5 _hFcEncodes DARPin-hFc E3_5 for mammalian expression and column purification
199618pcDNA3.1_27B3_hFcEncodes DARPin-hFc 27B3 for mammalian expression and column purification
199619pcDNA3.1_27F3_hFcEncodes DARPin-hFc 27F3 for mammalian expression and column purification

Antibodies from Article