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RabV CVS-N2c(deltaG)-mCherry
Plasmid#73464PurposeExpresses mCherry for monosynaptic circuit mappingDepositorInsertmCherry
UseNeurotropic virusTagsExpressionMutationPromoterAvailable sinceMarch 28, 2016AvailabilityAcademic Institutions and Nonprofits only -
PB-TO-hNGN2-BSD-mApple
Plasmid#182308PurposePiggybac Tet-ON plasmid for differentiating hiPSCs into glutamatergic neurons via NGN2 expression, bsd selection, mAppleInsertsUseTagsT2A-mycNLS-mAppleExpressionMammalianMutationPromoterEF1a and TRE3GAvailable sinceApril 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
RabV CVS-N2c(deltaG)-Cre
Plasmid#73474PurposeExpresses Cre for circuit mapping and genetic manipulationDepositorInsertCre recombinase
UseNeurotropic virusTagsExpressionMutationPromoterAvailable sinceMarch 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
pAAV-Sico-Red
Plasmid#84882PurposeCre-inducible shRNADepositorInsertSico-Red
UseAAVTagsmCherryExpressionMutationPromoterEF1a and CMVAvailable sinceFeb. 13, 2017AvailabilityAcademic Institutions and Nonprofits only -
RabV CVS-N2c(deltaG)-FlpO
Plasmid#73473PurposeExpresses FlpO for circuit mapping and genetic manipulationDepositorInsertFlpO
UseNeurotropic virusTagsExpressionMutationPromoterAvailable sinceMay 16, 2016AvailabilityAcademic Institutions and Nonprofits only -
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RabV CVS-N2c(deltaG)-GCaMP6f
Plasmid#73466PurposeExpresses GCaMP6f for calcium imagingDepositorInsertGCaMP6f
UseNeurotropic virusTagsExpressionMutationPromoterAvailable sinceMarch 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
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Pprm1-luciferase; his-3
Plasmid#137728PurposeCommunication-activated luciferase in Neurospora crassaDepositorInsertsLuciferase
Pprm-1 Promoter
UseLuciferase; Neurospora crassa expressionTagsExpressionMutationCodon-optimized for N. crassaPromoterAvailable sinceFeb. 14, 2020AvailabilityAcademic Institutions and Nonprofits only -
RabV CVS N2c(deltaG)-synaptophysin-GFP
Plasmid#73492PurposeExpresses EGFP for monosynaptic circuit mapping at the synapseDepositorInsertsynaptophysin-GFP
UseNeurotropic virusTagsExpressionMutationPromoterAvailable sinceMarch 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3 Glt1a
Plasmid#98705Purposemammalian expression of Glt1aDepositorInsertGlt1a (Slc1a2 Rat)
UseTagsExpressionMammalianMutationI288V (please see depositor comments below)PromoterCMVAvailable sinceJuly 17, 2017AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3 Glt1b
Plasmid#98706Purposemammalian expression of Glt1bDepositorInsertGlt1b (Slc1a2 Rat)
UseTagsExpressionMammalianMutationI348V and N365Y (please see depositor comments se…PromoterCMVAvailable sinceJuly 17, 2017AvailabilityAcademic Institutions and Nonprofits only -
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pCAG-ChroME2f-mRuby2-ST
Plasmid#170160PurposeCation channelrhodopsin ChroME2f fused to mRuby2 fluorophore and targeted to the neuronal soma and proximal dendritesDepositorInsertChroME2f-mRuby2-ST
UseTagsExpressionMammalianMutationPromoterAvailable sinceAug. 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCAG-ChRmine-mRuby2-ST
Plasmid#227777PurposeCation channelrhodopsin ChRmine fused to mRuby2 fluorophore and targeted to the neuronal soma and proximal dendrites for optogenetic excitationDepositorInsertChRmine-mRuby2-ST
UseTagsExpressionMammalianMutationPromoterAvailable sinceNov. 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLenti Lifeact-mRuby2 BlastR
Plasmid#84384PurposeLentiviral expression of mRuby2-tagged Lifeact with Blasticidin selection in cells including neuronsDepositorInsertLifeact
UseLentiviralTagsmRuby2ExpressionMutationPromoterCMV immediate earlyAvailable sinceJan. 5, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCAGGS-3xFLAG-Rbfox3
Plasmid#213727PurposeMammalian cell expression vector for overexpression of Rbfox3 in motor neurons.DepositorInsertRbfox3 (Rbfox3 Mouse)
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 4, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCAGGS-3xFLAG-Rbfox1
Plasmid#213725PurposeMammalian cell expression vector for overexpression of Rbfox1 in motor neurons.DepositorInsertRbfox1 (Rbfox1 Mouse)
UseTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
RabV CVS-N2c(deltaG)-mCherry-P2A-FlpO
Plasmid#73471PurposeExpresses mCherry and FlpO for monosynaptic circuit mapping and genetic manipulationDepositorInsertmCherry-P2A-FlpO
UseNeurotropic virusTagsExpressionMutationPromoterAvailable sinceJune 2, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCAGGS-3xFLAG-Rbfox2
Plasmid#213726PurposeMammalian cell expression vector for overexpression of Rbfox2 in motor neurons.DepositorInsertRbfox2 (Rbfox2 Mouse)
UseTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAM-AAV-mCamk2a-EGFP
Plasmid#153197PurposeMouse Camk2a (mCamk2a) promoter-mediates gene expression in retinal neurons with fluorescent reporterDepositorTypeEmpty backboneUseAAVTagsExpressionMammalianMutationPromoterAvailable sinceJuly 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAM-AAV-hSyn1-EGFP
Plasmid#153205PurposeHuman synapsin-1 (hSyn1) promoter-mediates gene expression in retinal neurons with fluorescent reporterDepositorTypeEmpty backboneUseAAVTagsExpressionMammalianMutationPromoterAvailable sinceJuly 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAM-AAV-mSyn1-EGFP
Plasmid#153187PurposeMouse synapsin-1 (mSyn1) promoter-mediates gene expression in retinal neurons with fluorescent reporterDepositorTypeEmpty backboneUseAAVTagsExpressionMammalianMutationPromoterAvailable sinceJune 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
RabV CVS-N2c(deltaG)-mCherry-P2A-Cre
Plasmid#73472PurposeExpresses mCherry and Cre for monosynaptic circuit mapping and genetic manipulationDepositorInsertmCherry-P2A-Cre
UseNeurotropic virusTagsExpressionMutationPromoterAvailable sinceMarch 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
pEY12
Plasmid#191018PurposeFluorescent reporter driving panneuronal nuclear GCaMP6s expression (refer to NeuroPAL paper for expression)DepositorInsertrab-3 (rab-3 Nematode)
UseTagsExpressionWormMutationNonePromoterAvailable sinceNov. 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
pFUGW-RapACR-EYFP
Plasmid#111075PurposeFast anion channelrhodopsin (current half-decay time 3-10 ms depending on voltage and light intensity) for time-resolved inhibition of neuronal spikingDepositorInsertAnion channelrhodopsin RapACR
UseLentiviralTagsEYFPExpressionMutationPromoterAvailable sinceJune 4, 2018AvailabilityAcademic Institutions and Nonprofits only -
pFUGW-RapACR_T111C-EYFP
Plasmid#119195PurposeFast anion channelrhodopsin mutant RapACR_T111C for time-resolved inhibition of neuronal spikingDepositorInsertAnion channelrhodopsin RapACR fast mutant T111C
UseLentiviralTagsEYFPExpressionMutationchanged threonine 111 to cysteinePromoterUbiquitinAvailable sinceDec. 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAM-AAV-CAG-EGFP
Plasmid#153186PurposeCAG promoter-mediates gene expression in retinal neurons with fluorescent reporterDepositorTypeEmpty backboneUseAAVTagsExpressionMammalianMutationPromoterAvailable sinceJune 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLV-75-2
Plasmid#73036PurposeExpress an NT-3-BDNF hybrid neurotrophic factor, with reduced affinity to p75NTR, in mammalian cellsDepositorInsertp75-2
UseLentiviralTagsExpressionMammalianMutationMature Human NT-3 gene exchanged Aspartic 15 to a…PromoterAvailable sinceApril 22, 2016AvailabilityAcademic Institutions and Nonprofits only -
RabV CVS-N2c(deltaG)-hChR2-YFP
Plasmid#73465PurposeExpresses channelrhodopsin fused to YFP for monosynaptic circuit mapping and optogenetic activationDepositorInserthChR2-YFP
UseNeurotropic virusTagsExpressionMutationPromoterAvailable sinceMarch 28, 2016AvailabilityAcademic Institutions and Nonprofits only -
pAAV-flex-rev-g2-2A-Venus
Plasmid#71410PurposeThis plasmid is pAAV-panpromoter-flex-rev-gamma2F77-2A-Venus. Confers zolpidem sensitivity on zolpidem-insensitive neurons.DepositorInsertsUseAAV and Cre/LoxTagsExpressionMammalianMutationPromotermouse hdc gene promoter, works as a pan neuronal …Available sinceMarch 1, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCRII-Topo S100a10 in situ probe
Plasmid#45603DepositorInserts100a10 in situ probe (S100a10 Mouse)
UseIn situTagsExpressionMutationfragment contains bp# 21-275 of BC025044PromoterAvailable sinceJuly 5, 2013AvailabilityAcademic Institutions and Nonprofits only -
pPB-tetpA-iRPT
Plasmid#140763PurposeExpresses iRFP713, PuroR and rtTAM2 in mammalian cells, with a TRE cassetteDepositorInsertsiRFP713
PuroR
rtTAM2
UseTagsExpressionMammalianMutationPromoterAvailable sinceSept. 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCRII-Topo Crym in situ probe
Plasmid#45601DepositorInsertCrym in situ probe (Crym Mouse)
UseIn situTagsExpressionMutationfragment contains nt 756-1191 of NM_016669PromoterAvailable sinceJune 3, 2013AvailabilityAcademic Institutions and Nonprofits only -
pWPXL-resNP1
Plasmid#65061PurposeConstitutive expression of NP1 resistant to silencing by RNAi with pLVTH-shNP1DepositorInsertNeuronal Pentraxin 1 (Nptx1 Rat)
UseLentiviralTagsExpressionMammalianMutationG447A, C450T, A451T, G452C, C454A, C456A, C459GPromoterEF1alphaAvailable sinceOct. 1, 2015AvailabilityAcademic Institutions and Nonprofits only -
pAM-AAV-hIsl2-EGFP
Plasmid#153206PurposeHuman Isl2 (hIsl2) promoter-mediates gene expression in retinal neurons with fluorescent reporterDepositorTypeEmpty backboneUseAAVTagsExpressionMammalianMutationPromoterAvailable sinceJune 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCAG-ChroMD-mRuby2-ST
Plasmid#171158PurposeCation channelrhodopsin ChroMD fused to mRuby2 fluorophore and targeted to the neuronal soma and proximal dendritesDepositorInsertChroMD
UseTagsExpressionMammalianMutationPromoterAvailable sinceAug. 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAM-AAV-mIsl2-EGPF
Plasmid#153191PurposeMouse Isl2 (mIsl2) promoter-mediates gene expression in retinal neurons with fluorescent reporterDepositorTypeEmpty backboneUseAAVTagsExpressionMammalianMutationPromoterAvailable sinceJune 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
pENTR CMVie-Lifeact-mRuby2 L1-R5
Plasmid#84389PurposeMultisite Gateway entry vector for expression of mRuby2-tagged Lifeact in cells including neuronsDepositorInsertLifeact
UseGatewayTagsmRuby2ExpressionMammalianMutationPromoterCMV immediate earlyAvailable sinceJan. 5, 2017AvailabilityAcademic Institutions and Nonprofits only -
pMTL3
Plasmid#127966Purposeconstitutive expression of KRAB-dCas9-BFP-KRAB from the AAVS1 locusDepositorInsertAAVS1-CAG-KRAB-dCas9-BFP-KRAB
UseTALENTagsExpressionMammalianMutationPromoterCAGAvailable sinceNov. 23, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMTL5
Plasmid#127967PurposeDHFR-degron controlled expression of KRAB-dCas9-BFP-KRAB from the AAVS1 locusDepositorInsertAAVS1-CAG-DHFR-KRAB-dCas9-BFP-KRAB
UseTALENTagsExpressionMammalianMutationPromoterCAGAvailable sinceNov. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
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H52-pUCM-hNGN2-inv
Plasmid#110492PurposeDonor construct for introduction of NGN2 into AAVS1 safe harbor site and iPSC differentiation to cortical neuron, TALEN cut site outside of homology armDepositorInsertsUseCRISPR, Cre/Lox, and TALENTagsExpressionMammalianMutationPromoterCAG, EF-1 alpha, and TRE3GAvailable sinceJune 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn-Flex-mRuby3-2A
Plasmid#203846PurposeNeuron specific expression of a Cre-dependent mRuby3DepositorInsertmRuby3
UseAAVTagsExpressionMutationPromoterAvailable sinceAug. 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
AAV-hSyn-FlicR1
Plasmid#74143PurposeExpress FlicR1 preferentially in neuronsDepositorInsertFlicR1
UseAAVTagsExpressionMammalianMutationPromoterhSynAvailable sinceApril 19, 2016AvailabilityAcademic Institutions and Nonprofits only -
pLV-NT-3/D15A
Plasmid#73035PurposeExpress an NT-3-BDNF hybrid neurotrophic factor in mammalian cellsDepositorInsertNT-3/D15A (NTF3 Human)
UseLentiviralTagsExpressionMammalianMutationMutated Aspartic acid in position 15 of the matur…PromoterAvailable sinceApril 22, 2016AvailabilityAcademic Institutions and Nonprofits only -
pAM-AAV-hSncg-EGFP
Plasmid#153198PurposeHuman gamma-synuclein (hSncg) promoter-mediates gene expression in retinal neurons with fluorescent reporterDepositorTypeEmpty backboneUseAAVTagsExpressionMammalianMutationPromoterAvailable sinceJuly 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCRII-Topo Diap3 in situ probe
Plasmid#45602DepositorInsertDiap3 in situ probe (Diaph3 Mouse)
UseIn situTagsExpressionMutationfragment contains bp# 1589-2321 of BC028920PromoterAvailable sinceJuly 5, 2013AvailabilityAcademic Institutions and Nonprofits only -
pCRII-Topo Crim1 in situ probe
Plasmid#45600DepositorInsertCrim1 in situ probe (Crim1 Mouse)
UseIn situTagsExpressionMutationinsert contains bp#4062-4502 of NM_015800.3PromoterAvailable sinceJuly 5, 2013AvailabilityAcademic Institutions and Nonprofits only
