pLV-NT-3/D15A
(Plasmid
#73035)
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PurposeExpress an NT-3-BDNF hybrid neurotrophic factor in mammalian cells
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 73035 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepRRLsinPPT.CMV.MCS.Wpre
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Backbone manufacturerNaldini
- Backbone size w/o insert (bp) 6700
- Total vector size (bp) 7600
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Vector typeMammalian Expression, Lentiviral
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Growth instructionsGrow on low salt, 5gm, of LB
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameNT-3/D15A
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SpeciesH. sapiens (human)
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Insert Size (bp)800
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MutationMutated Aspartic acid in position 15 of the mature NT-3 to alanine. With this mutation NT-3 can bind also TrkB.
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Entrez GeneNTF3 (a.k.a. HDNF, NGF-2, NGF2, NT-3, NT3)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site XbaI and BamHI (not destroyed)
- 3′ cloning site none (destroyed during cloning)
- 5′ sequencing primer cmv_frw_primer (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pLV-NT-3/D15A was a gift from Pantelis Tsoulfas (Addgene plasmid # 73035 ; http://n2t.net/addgene:73035 ; RRID:Addgene_73035) -
For your References section:
A multifunctional neurotrophin with reduced affinity to p75NTR enhances transplanted Schwann cell survival and axon growth after spinal cord injury. Enomoto M, Bunge MB, Tsoulfas P. Exp Neurol. 2013 Oct;248:170-82. doi: 10.1016/j.expneurol.2013.06.013. Epub 2013 Jun 20. 10.1016/j.expneurol.2013.06.013 PubMed 23792206