RabV CVS-N2c(deltaG)-mCherry-P2A-FlpO
(Plasmid
#73471)
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PurposeExpresses mCherry and FlpO for monosynaptic circuit mapping and genetic manipulation
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 73471 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backboneRabies CVS N2c(deltaG)
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Backbone manufacturerMatthias J. Schnell, Thomas Jefferson University
- Backbone size w/o insert (bp) 14070
- Total vector size (bp) 16116
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Vector typeNeurotropic virus
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Growth instructionsCan also be grown in Stbl2 or Stbl3
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Copy numberLow Copy
Gene/Insert
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Gene/Insert namemCherry-P2A-FlpO
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Insert Size (bp)2055
Resource Information
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Articles Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
RabV CVS-N2c(deltaG)-mCherry-P2A-FlpO was a gift from Thomas Jessell (Addgene plasmid # 73471 ; http://n2t.net/addgene:73471 ; RRID:Addgene_73471) -
For your References section:
Rabies Virus CVS-N2c Strain Enhances Retrograde Synaptic Transfer and Neuronal Viability. Reardon TR, Murray AJ, Turi GF, Wirblich C, Croce KR, Schnell MJ, Jessell TM, Losonczy A. Neuron. 2016 Jan 19. pii: S0896-6273(16)00005-2. doi: 10.1016/j.neuron.2016.01.004. 10.1016/j.neuron.2016.01.004 PubMed 26804990