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AAV2/9 REP-AAP-ΔCAP

Plasmid

#205991

Purpose

Rep and AAP protein expression for AAV CREATE libraries

Depositor

Viviana Gradinaru

Article

Chuapoco et al Nat Nanotechnol. 2023 Jul 10. doi: 10.10

Insert

AAV9 Rep and AAP proteins for CREATE libraries

Use

AAV

Tags

Expression

Mutation

Promoter

Available since

Aug. 18, 2023

Availability

Academic Institutions and Nonprofits only

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AAV pmSyn1-EBFP-Cre

Plasmid

#51507

Purpose

Can be used to generate AAV virus that will express Cre in neurons from the synapsin promoter

Depositor

Hongkui Zeng

Article

Madisen et al Neuron. 2015 Mar 4;85(5):942-58. doi: 10

Has Service

AAV5

Insert

EBFP-Cre

Use

AAV

Tags

Expression

Mutation

Promoter

pmSyn1

Available since

July 14, 2014

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn-DIO-GRAB_ACh3.0

Plasmid

#121923

Purpose

express the genetically-encoded fluorescent acethycholine(ACh) sensor GRAB-ACh3.0 in a cre-dependent manner

Depositor

Yulong Li

Article

Jing et al Nat Methods. 2020 Sep 28. pii: 10.1038/s

Insert

GPCR activation based ACh sensor GRAB-ACh3.0

Use

AAV

Tags

Expression

Mutation

Promoter

hSyn

Available since

Jan. 16, 2020

Availability

Academic Institutions and Nonprofits only

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pAAV-U6-gRNA-CBh-mCherry

Plasmid

#91947

Purpose

Expression of empty gRNA cassette and mCherry

Depositor

Jimok Kim

Article

Li et al Neuroscience. 2017 Sep 6. pii: S0306-452

Insert

mCherry

Use

AAV

Tags

Expression

Mutation

Promoter

Available since

Sept. 12, 2017

Availability

Academic Institutions and Nonprofits only

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pAAVS1-Puro-CAG-mNG2(1-10)

Plasmid

#206042

Purpose

Repair template for the integration of a mNG2(1-10) expression cassette into the AAVS1 locus in human cells.

Depositor

Alisa Piekny

Article

Husser et al Elife. 2024 Apr 23;12:RP92819. doi: 10.7

Insert

AAVS1 homology arms with SA-P2A-Puro marker and CAG-mNG2(1-10) expression cassette

Use

CRISPR and Synthetic Biology; Donor template

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 23, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV-S5E2-C1V1-eYFP

Plasmid

#135633

Purpose

AAV vector to drive the expression of C1V1-eYFP in PV cortical interneuronsunder the control of the E2 regulatory element

Depositor

Jordane Dimidschstein

Article

Vormstein-Schneider et al Nat Neurosci. 2020 Dec;23(12):1629-1636.

Has Service

AAV PHP.eB, AAV1, AAV5, and AAV9

Insert

C1V1-eYFP

Use

AAV

Tags

Expression

Mutation

None

Promoter

Available since

Aug. 17, 2020

Availability

Academic Institutions and Nonprofits only

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pAAV-EF1alpha-soCoChR-tdTomato

Plasmid

#107714

Purpose

A somatic channelrhodopsin, which enables single cell, single millisecond resolution optogenetics. EF1alpha promoter

Depositor

Edward Boyden

Article

Shemesh et al Nat Neurosci. 2017 Dec;20(12):1796-1806.

Insert

soCoChR-tdTomato

Use

AAV

Tags

KA2 and tdTomato

Expression

Mammalian

Mutation

Promoter

EF1alpha

Available since

April 30, 2018

Availability

Academic Institutions and Nonprofits only

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pAAV-Ef1a-DIO EYFP

Plasmid

#27056

Depositor

Karl Deisseroth

Has Service

AAV Retrograde, AAV1, AAV2, AAV5, and AAV9

Insert

EYFP

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 27, 2011

Availability

Academic Institutions and Nonprofits only

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pAAV-CBA-DO(FAS)-GCaMP6s

Plasmid

#110135

Purpose

Cre-off expression of GCaMP6s

Depositor

Bernardo Sabatini

Article

Markowitz et al Cell. 2018 May 10. pii: S0092-8674(18)30

Insert

GCaMP6s

Use

AAV and Cre/Lox

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 8, 2018

Availability

Academic Institutions and Nonprofits only

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AAV_NLS-dSaCas9-NLS-VPR

Plasmid

#68495

Purpose

AAV vector containing nuclease null SaCas9 fused to VPR

Depositor

George Church

Article

Kiani et al Nat Methods. 2015 Sep 7. doi: 10.1038/nm

Insert

dSaCas9

Use

AAV

Tags

VPR

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 11, 2015

Availability

Academic Institutions and Nonprofits only

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AAV-CAG-ChR2-GFP

Plasmid

#26929

Purpose

AAV-mediated expression of ChR2-GFP for neuronal excitation

Depositor

Edward Boyden

Article

Boyden et al Nat Neurosci. 2005 Sep . 8(9):1263-8.

Insert

ChR2

Use

AAV; Adeno-associated virus

Tags

GFP

Expression

Mammalian

Mutation

Promoter

CAG

Available since

Jan. 11, 2011

Availability

Academic Institutions and Nonprofits only

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pC129: pAAV.CMV-CasRx mCh

Plasmid

#203442

Purpose

Plasmid expressing active RfxCas13d with mCherry reporter for investigating CasRx activity

Depositor

Guei-Sheung Liu

Article

Kumar et al Proc Natl Acad Sci U S A. 2024 Nov 5;121

Insert

RfxCas13d-T2A-mCherry

Use

AAV and CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Dec. 12, 2024

Availability

Academic Institutions and Nonprofits only

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AAV-CamKIIa-jGCaMP8m-WPRE

Plasmid

#176751

Purpose

AAV-mediated expression of GCaMP8m under the CamKIIa promoter

Depositor

Loren Looger

Has Service

AAV Retrograde, AAV1, AAV5, and AAV9

Insert

jGCaMP8m

Use

AAV

Tags

6xHis

Expression

Mammalian

Mutation

Promoter

CamKIIalpha

Available since

Nov. 30, 2021

Availability

Academic Institutions and Nonprofits only

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pAAV.CAG.(cyto).iATPSnFR2.A95A.A119L.HaloTag

Plasmid

#209653

Purpose

Expression of iATPSnFR2, low affinity

Depositor

Tim Brown, HHMI-JRC Tool Translation Team

Article

Marvin et al Proc Natl Acad Sci U S A. 2024 May 21;12

Insert

iATPSnFR2.A95A.A119L.HaloTag

Use

AAV

Tags

Expression

Mutation

A95A.A119L

Promoter

CAG

Available since

Nov. 3, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV-R26-FLEX-mStr

Plasmid

#135619

Purpose

Donor vector for genomic targeting of a CAG-FLEX-mStrawberry cassette to the mouse Rosa26 locus

Depositor

Lin He

Article

Chen et al Cell Rep. 2019 Jun 25;27(13):3780-3789.e

Insert

CAG-FLEX-mStrawberry flanked by Rosa26 homology arms (Gt(ROSA)26Sor Mouse)

Use

AAV, CRISPR, and Mouse Targeting

Tags

Expression

Mutation

Promoter

Available since

May 18, 2020

Availability

Academic Institutions and Nonprofits only

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pAAV-nEF-ChRmine-mScarlet

Plasmid

#137158

Purpose

Viral expression of ChRmine-p2a-mScarlet

Depositor

Karl Deisseroth, INTRSECT 2.0 Project

Article

Fenno et al Neuron. 2020 Jun 20. pii: S0896-6273(20)

Has Service

AAV8

Insert

ChRmine-p2a-mScarlet

Use

AAV

Tags

Expression

Mutation

n/a

Promoter

nEF

Available since

June 25, 2020

Availability

Academic Institutions and Nonprofits only

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Cbh_v5 AAV-ABE C-terminal

Plasmid

#137178

Purpose

AAV genome: expresses the C-terminal of v5 AAV-ABE from the Cbh promoter, and U6-sgRNA

Depositor

David Liu

Article

Levy et al Nat Biomed Eng. 2020 Jan;4(1):97-110. do

Insert

v5 AAV-CBE C-terminal; U6-protospacer

Use

AAV

Tags

Expression

Mutation

Promoter

CMV

Available since

Jan. 30, 2020

Availability

Academic Institutions and Nonprofits only

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pAAV-flex-taCasp3-TEVp

Plasmid

#45580

Depositor

Nirao Shah, Jim Wells

Article

Yang et al Cell. 2013 May 9;153(4):896-909. doi: 10

Has Service

AAV5

Inserts

Caspase 3 (CASP3 Synthetic, Human)
Tobacco Etch Virus protease

Use

AAV; Adeno associated viral vector

Tags

Expression

Mutation

Linker replaced with a TEV protease cleavage site

Promoter

EF1a

Available since

July 10, 2013

Availability

Academic Institutions and Nonprofits only

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AAV-SYN-mBaoJin-oPRE

Plasmid

#218683

Purpose

AAV vector plasmid expressing mBaoJin under the human synapsin (SYN) promoter. It also contains an optimised and truncated version of WPRE as a regulatory element.

Depositor

Bart Nieuwenhuis

Insert

mBaoJin

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

Human synapsin promoter

Available since

Oct. 23, 2024

Availability

Academic Institutions and Nonprofits only

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Cbh_v5 AAV-ABE N-terminal

Plasmid

#137177

Purpose

AAV genome: expresses the N-terminal of v5 AAV-ABE from the Cbh promoter

Depositor

David Liu

Article

Levy et al Nat Biomed Eng. 2020 Jan;4(1):97-110. do

Insert

v5 AAV-ABE N-terminal

Use

AAV

Tags

Expression

Mutation

Cas9 D10A

Promoter

Cbh

Available since

Jan. 30, 2020

Availability

Academic Institutions and Nonprofits only

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