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PurposeExpression of empty gRNA cassette and mCherry
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 91947 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backboneAAV:ITR-U6-sgRNA(backbone)-pCBh-Cre-WPRE-hGHpA-ITR
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Backbone manufacturerF. Zhang Lab (Addgene #60229)
- Backbone size w/o insert (bp) 6394
- Total vector size (bp) 6025
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Modifications to backboneThe Cre gene in the backbone plasmid was replaced by the mCherry gene at the restriction sites AgeI and EcoRI. The mCherry DNA was PCR-amplified from the plasmid pAAV-CaMKIIa-hChR2(E123T/T159C)-mCherry (Addgene #35512).
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Vector typeAAV
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberUnknown
Gene/Insert
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Gene/Insert namemCherry
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SpeciesSynthetic
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Insert Size (bp)711
Resource Information
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Articles Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pAAV-U6-gRNA-CBh-mCherry was a gift from Jimok Kim (Addgene plasmid # 91947 ; http://n2t.net/addgene:91947 ; RRID:Addgene_91947) -
For your References section:
Distinct roles of neuronal and microglial CB2 cannabinoid receptors in the mouse hippocampus. Li Y, Kim J. Neuroscience. 2017 Sep 6. pii: S0306-4522(17)30629-2. doi: 10.1016/j.neuroscience.2017.08.053. 10.1016/j.neuroscience.2017.08.053 PubMed 28888955