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Transgenic mice for intersectional targeting of neural sensors and effectors with high specificity and performance.

Madisen L, Garner AR, Shimaoka D, Chuong AS, Klapoetke NC, Li L, van der Bourg A, Niino Y, Egolf L, Monetti C, Gu H, Mills M, Cheng A, Tasic B, Nguyen TN, Sunkin SM, Benucci A, Nagy A, Miyawaki A, Helmchen F, Empson RM, Knopfel T, Boyden ES, Reid RC, Carandini M, Zeng H
Neuron. 2015 Mar 4;85(5):942-58. doi: 10.1016/j.neuron.2015.02.022. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
50363AAV phSyn1(S)-DreO-bGHpACan be used to generate AAV virus that will express DreO recombinase in neurons from the synapsin promoter
50364AAV phSyn1(S)-FlpE-bGHpACan be used to generate AAV virus that will express FlpE recombinse gene in neurons from the synapsin promoter
51507AAV pmSyn1-EBFP-CreCan be used to generate AAV virus that will express Cre in neurons from the synapsin promoter
51669AAV phSyn1(S)-FlpO-bGHpACan be used to generate AAV virus that will express the FlpO recombinase gene in neurons from the synapsin promoter.
61570Pvalb-2A-Cre targeting vectorTarget the Cre recombinase gene to the stop codon of the mouse Pvalb gene
61571Pvalb-2A-Dre Flp-in replacement vectorRecombinase-mediated cassette exchange in ES cells to insert the Dre recombinase gene at the mouse Pvalb gene stop codon
61572Pvalb-2A-Flpo Flp-in replacement vectorRecombinase-mediated cassette exchange in ES cells to insert the Flpo recombinase gene at the mouse Pvalb gene stop codon
61573Rasgrf2-2A-dCre targeting vectorTarget a DHFR-destabilized Cre recombinase gene to the stop codon of the mouse Rasgrf2 gene
61574Slc17a7-IRES2-Cre targeting vectorTarget the Cre recombinase gene to the stop codon of the mouse Slc17a7 (VGlut1) gene
61575Snap25-LSL-2A-GFP targeting vectorTarget a Cre-dependent GFP expression cassette to the stop codon of the mouse Snap25 gene
61576Ai62(TITL-tdT) Flp-in replacement vectorRecombinase-mediated cassette exchange in ES cells to insert a Cre & tTA-dependent tdTomato expression cassette into a docking site within the mouse TIGRE genomic locus
61577Ai65(RCFL-tdT) targeting vectorTarget a Flp and Cre-dependent tdTomato expression cassette to the mouse Rosa26 locus
61578Ai66(RCRL-tdT) targeting vectorTarget a Dre and Cre-dependent tdTomato expression cassette to the mouse Rosa26 locus
61579Ai95(RCL-GCaMP6f) targeting vectorTarget a Cre-dependent GCaMP6-fast expression cassette to the mouse Rosa26 locus
61580TIGRE docking site targeting vectorCreate a docking site in the mouse TIGRE genomic locus that allows modification in ES cells by Flp recombinase-mediated cassette exchange
65429AAV phSyn1-RSR-FLEX-ChR2(H134R)-EYFP-WPRE-bGHpACan be used to generate AAV virus that will express the ChR2(H134R)-EYFP channelrhodopsin fusion protein in neurons under intersectional control by Dre and Cre recombinases
65449AAV pTRE-FLEX-EGFP-WPRE-bGHpACan be used to generate AAV virus that will express EGFP from the tetO promoter in a Cre-dependent manner
65453AAV pTRE-FSF-FLEX-EGFP-WPRE-bGHpACan be used to generate AAV virus that will express EGFP from the tetO promoter under intersectional control by Flp and Cre recombinases
65454AAV phSyn1-FSF-FLEX-ChR2(H134R)-EYFP-WPRE-bGHpACan be used to generate AAV virus that will express the ChR2(H134R)-EYFP channelrhodopsin fusion protein in neurons under intersectional control by Flp and Cre recombinases
65455AAV pCAG-FSF-FLEX-EGFP-WPRE-bGHpACan be used to generate AAV virus that will express EGFP from the CAG promoter under intersectional control by Flp and Cre recombinases
65458AAV pCAG-FLEX2-tTA2-WPRE-bGHpACan be used to generate AAV virus that will express the tetracycline transactivator tTA2 from the CAG promoter in a Cre-dependent manner

Antibodies from Article