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Showing: 2881 - 2920 of 149167 results

  1. LentiGuide Puro-P2A-EGFP

    Plasmid
    #137729
    Purpose
    For simple determination of the multiplicity of infection (MOI) of a lentiviral CRISPR library by checking EGFP expression (still allowing for puro selection).
    Depositor
    Fredrik Wermeling
    Article
    Panda et al Proc Natl Acad Sci U S A. 2020 Jan 24. p
    Type
    Empty backbone
    Use
    CRISPR, Lentiviral, and Synthetic Biology
    Tags
    EGFP
    Expression
    Bacterial and Mammalian
    Mutation
    Promoter
    Available since
    Feb. 21, 2020
    Availability
    Academic Institutions and Nonprofits only
    137729-plasmid-map-sequence-id-272646

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  4. pCI pHluorin Syt1

    Plasmid
    #184502
    Purpose
    Mammalian expression of Synaptotagmin 1 with pH-sensitive fluorescent protein
    Depositor
    James Rothman
    Insert
    Synaptotagmin 1 (Syt1 Rat)
    Use
    Tags
    Super Ecliptic pHluorin
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    May 15, 2023
    Availability
    Academic Institutions and Nonprofits only
    184502-plasmid-map-sequence-id-393122

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  11. dCas9-hHDAC3

    Plasmid
    #98591
    Purpose
    3rd generation lenti vector encoding dCas9-hHDAC3 (EF1a-NLS-dCas9(N863)-hHDAC3). Expresses dCas9 fused to human HDAC3.
    Depositor
    Zhaolan Zhou
    Article
    Kwon et al Nat Commun. 2017 May 12;8:15315. doi: 10
    Insert
    dCAS9-HDAC3 (HDAC3 Synthetic, S. pyogenes, Human)
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    D10A and N863A in Cas9
    Promoter
    EF1A
    Available since
    Oct. 3, 2017
    Availability
    Academic Institutions and Nonprofits only
    98591-plasmid-map-sequence-id-339879

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  13. CMV-hEAAT2

    Plasmid
    #32814
    Depositor
    Susan Amara
    Insert
    hEAAT2 (SLC1A2 Human)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Dec. 5, 2011
    Availability
    Academic Institutions and Nonprofits only
    32814-plasmid-map-sequence-id-258966

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  18. PB-CAG-DDdCas9VPH-T2A-GFP-IRES-Neo

    Plasmid
    #102886
    Purpose
    PiggyBac transposon system construct with constitutive CAG promoter expression of TMP inducible DDdCas9VP192-p65-HSF1 activator followed by T2A-EGFP as a reporter and IRES-Neo as selection
    Depositor
    Timo Otonkoski
    Article
    Weltner et al Nat Commun. 2018 Jul 6;9(1):2643. doi: 1
    Insert
    DDdCas9VP192-p65-HSF1-T2A-GFP-IRES-Neo
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    D10A, H840A
    Promoter
    CAG
    Available since
    July 10, 2018
    Availability
    Academic Institutions and Nonprofits only
    102886-plasmid-map-sequence-id-194712

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  20. pLKO.3G_X7

    Plasmid
    #171213
    Purpose
    3rd gen lentiviral backbone for cloning and expression of new shRNA sequences. Uses eGFP for selection. Please see cloning information for suggested restriction sites
    Depositor
    Jenny Wang
    Insert
    N/A; empty backbone
    Use
    Lentiviral and RNAi
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    July 22, 2021
    Availability
    Academic Institutions and Nonprofits only
    171213-plasmid-map-sequence-id-338282

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  22. pTP1172

    Plasmid
    #104161
    Purpose
    Bacterial expression plasmid of anti-mouse kappa chain nanobody TP1170 (3x Cysteines)
    Depositor
    Dirk Görlich
    Article
    Pleiner et al J Cell Biol. 2018 Mar 5;217(3):1143-1154
    Insert
    Anti-mouse kappa chain specific nanobody TP1170 (3xCysteines)
    Use
    Tags
    14xHistidine tag and NEDD8 from Brachypodium dist…
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Dec. 21, 2017
    Availability
    Academic Institutions and Nonprofits only
    104161-plasmid-map-sequence-id-200072

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  23. pSBtet-GP

    Plasmid
    #60495
    Purpose
    SB-transposon with inducible SfiI cloning site for GOI (contains firefly luciferase) and constitutive expression of GFP, rtTA and puromycin resistance gene
    Depositor
    Eric Kowarz
    Article
    Kowarz et al Biotechnol J. 2015 Feb 4. doi: 10.1002/b
    Has Service
    Cloning Grade DNA
    Type
    Empty backbone
    Use
    Transposon
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    TCE & RPBSA
    Available since
    Feb. 11, 2015
    Availability
    Academic Institutions and Nonprofits only
    60495-plasmid-map-sequence-id-237935

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  32. pLenti.CAG.H2B-cerFP-2A-mito-dsRFP.W

    Plasmid
    #51013
    Purpose
    Lentivector that expresses H2B-cerFP-2A-mito-dsRFP from internal CAG enhancer-promoter
    Depositor
    Rusty Lansford
    Insert
    H2B-cerFP-2A-mito-RFP
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CAG
    Available since
    June 2, 2014
    Availability
    Academic Institutions and Nonprofits only
    51013-plasmid-map-sequence-id-77778

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  39. pJC53.2

    Plasmid
    #26536
    Purpose
    T/A Cloning Vector for dsRNA generation and the generation of sense and anti-sense probes
    Depositor
    Phillip Newmark
    Article
    Collins et al PLoS Biol. 2010 . 8(10):e1000509.
    Type
    Empty backbone
    Use
    T/a cloning vector for dsrna generation and the g…
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Dec. 1, 2010
    Availability
    Academic Institutions and Nonprofits only
    26536-plasmid-map-sequence-id-12556

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Showing: 2881 - 2920 of 149167 results