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Continuous directed evolution of aminoacyl-tRNA synthetases.

Bryson DI, Fan C, Guo LT, Miller C, Soll D, Liu DR
Nat Chem Biol. 2017 Oct 16. doi: 10.1038/nchembio.2474. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
99147pDB007(+)Accessory plasmid for PACE of MjTyrRS variants
99209pDB021CH(+)Accessory plasmid for PACE of PylRS variants
99210pDB038Accessory plasmid for PACE of PylRS variants
99211pDB038aAccessory plasmid for PACE of PylRS variants
99212pDB038bAccessory plasmid for PACE of PylRS variants
99214pDB007ns2aAccessory plasmid for PACE of PylRS variants; negative selection
99215pDB036aAccessory plasmid for PACE of MjTyrRS variants; negative selection
99216pDB036dAccessory plasmid for PACE of MjTyrRS variants; negative selection
99217pDB023fComplementary plasmid for aaRS PACE; amber codons in T7 RNAP
99218pDB023f1Complementary plasmid for aaRS PACE; amber codons in T7 RNAP
99219pDB023kComplementary plasmid for aaRS PACE; amber codons in T7 RNAP
99220pDB016Accessory plasmid for aaRS PACE; negative selection
99222pTECH-chPylRS(IPYE)Expression plasmid encoding chPylRS(IPYE) and pylT
99397pDB070.iodo.5Expression plasmid encoding the evolved MjTyrRS variant Iodo.5RS for amber suppression with p-iodophenylalanine
104069pTECH-chAcK3RS(IPYE)Expression plasmid encoding chAcK3RS(IPYE) and pylT
104070pTECH-MbAcK3RS(IPYE)Expression plasmid encoding MbAcK3RS(IPYE) and pylT
104071pTECH-MmAcK3RS(IPYE)Expression plasmid encoding MmAcK3RS(IPYE) and pylT
104072pTECH-MbPylRS(IPYE)Expression plasmid encoding MbPylRS(IPYE) and pylT
104073pTECH-MmPylRS(IPYE)Expression plasmid encoding MmPylRS(IPYE) and pylT
109260pDB026aAccessory plasmid for PACE of MjTyrRS variants
109261pDB026eAccessory plasmid for PACE of MjTyrRS variants
109263pDB026gAccessory plasmid for PACE of MjTyrRS variants

Antibodies from Article