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Transcription activation is enhanced by multivalent interactions independent of phase separation.
Trojanowski J, Frank L, Rademacher A, Mucke N, Grigaitis P, Rippe K
Mol Cell. 2022 May 4. pii: S1097-2765(22)00328-8. doi: 10.1016/j.molcel.2022.04.017. PubMed Article

Plasmids from Article

ID Plasmid Purpose
183916rTetR-GFPReverse Tet repressor labeled with GFP; binds tetO sites upon doxycycline addition
183917rTetR-GFP-VP16VP16 activation domain coupled to reverse Tet repressor; labeled with GFP; binds tetO sites upon doxycycline addition
183918rTetR-GFP-VPRVPR activation domain coupled to reverse Tet repressor; labeled with GFP; binds tetO sites upon doxycycline addition
183919CIBN-rTetROptogenetic CIBN localizer domain coupled to reverse Tet repressor; binds tetO sites upon doxycycline addition; CIBN is bound by PHR upon blue light exposure
183920GFP-LacILac repressor labeled with GFP; can be used to label lacO sites
183921tdTomato-LacILac repressor labeled with tdTomato; can be used to label lacO sites
183922SNAPtag-LacILac repressor labeled with SNAPtag; can be used to label lacO sites
183923NLS-dCas9-HA-NLS-GFP-VP16VP16 activation domain coupled to catalytically inactive dCas9 for localization; labeled with GFP
183924NLS-dCas9-HA-NLS-GFP4-VPRVPR activation domain coupled to catalytically inactive dCas9 for localization; contains a GFP tetramer fpr labeling and as a spacer
183925NLS-dCas9-HA-NLS--GFP-p300p300 HAT core domain coupled to catalytically dead dCas9 for localization; labeled with GFP
183926PHR-GFPOptogenetic PHR domain coupled to GFP; binds to CIBN upon blue light exposure
183927PHR-GFP-VP16Optogenetic PHR domain coupled to GFP and VP16 activation domain; binds to CIBN upon blue light exposure
183928PHR-GFP-VPROptogenetic PHR domain coupled to GFP and VPR activation domain; binds to CIBN upon blue light exposure
183929PHR-GFP-p65Optogenetic PHR domain coupled to GFP and p65 activation domain; binds to CIBN upon blue light exposure
183930PHR-GFP-RtaOptogenetic PHR domain coupled to GFP and Rta activation domain; binds to CIBN upon blue light exposure
183931PHR-GFP-STAT2Optogenetic PHR domain coupled to GFP and STAT2 activation domain; binds to CIBN upon blue light exposure
183932PHR-GFP-FUSNOptogenetic PHR domain coupled to GFP and FUSN domain with a high phase separation propensity; binds to CIBN upon blue light exposure
183933PHR-GFP-FUSN-VP16Optogenetic PHR domain coupled to GFP, FUSN (high phase separation propensity) and VP16 activation domain; binds to CIBN upon blue light exposure
183934NLS-tdPCP-GFPPCP tandem dimer binding to PP7 RNA stem loops; labeled with GFP
183935NLS-tdPCP-GFP-VP16PCP tandem dimer binding to PP7 RNA stem loops coupled to VP16 activation domain; labeled with GFP
183936NLS-tdPCP-GFP-VPRPCP tandem dimer binding to PP7 RNA stem loops coupled to VP16 activation domain; labeled with GFP
183937NLS-tdPCP-CIBNPCP tandem dimer binding to PP7 RNA stem loops coupled to optogenetic CIBN domain; bound by PHR upon blue light exposure
183938NLS-HA-tdMCP-tdTomatotandem MS2 binding protein labeled with tdTomato, containing a NLS and a HA-tag
183939mCherry-BRD4Transcriptional co-activator BRD4 labeled by mCherry

Antibodies from Article