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Purposevisualization of mitochondria
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 49151 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepAcGFP1-N1
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Backbone manufacturerClontech
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Modifications to backboneGFP was replaced with TagBFP using BamHI/NotI sites.
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Vector typeMammalian Expression
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberLow Copy
Gene/Insert
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Gene/Insert namemitochondrial targeting signal
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Alt nameCOX4, aa1-21
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SpeciesS. cerevisiae (budding yeast)
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Entrez GeneCOX4 (a.k.a. YGL187C)
- Promoter CMV
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Tag
/ Fusion Protein
- BFP (C terminal on backbone)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site XhoI (not destroyed)
- 3′ cloning site BamHI (not destroyed)
- 5′ sequencing primer CMV-F (Common Sequencing Primers)
Resource Information
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Articles Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
mito-BFP was a gift from Gia Voeltz (Addgene plasmid # 49151 ; http://n2t.net/addgene:49151 ; RRID:Addgene_49151) -
For your References section:
ER tubules mark sites of mitochondrial division. Friedman JR, Lackner LL, West M, DiBenedetto JR, Nunnari J, Voeltz GK. Science. 2011 Oct 21;334(6054):358-62. doi: 10.1126/science.1207385. Epub 2011 Sep 1. 10.1126/science.1207385 PubMed 21885730