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Addgene

Rtn4a-GFP
(Plasmid #61807)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 61807 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pAcGFP1-N1
  • Backbone manufacturer
    Clontech
  • Backbone size w/o insert (bp) 4700
  • Total vector size (bp) 8279
  • Modifications to backbone
    None
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    XL1 Blue
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Rtn4a
  • Alt name
    NOGO-A
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    3579
  • Mutation
    Rtn4a Stop Codon removed
  • GenBank ID
    NM_020532.4
  • Entrez Gene
    RTN4 (a.k.a. ASY, NI220/250, NOGO, NSP, NSP-CL, Nbla00271, Nbla10545, RTN-X, RTN4-A, RTN4-B1, RTN4-B2, RTN4-C)
  • Promoter CMV
  • Tag / Fusion Protein
    • GFP (C terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site XhoI (not destroyed)
  • 3′ cloning site KpnI (not destroyed)
  • 5′ sequencing primer None
  • 3′ sequencing primer None
  • (Common Sequencing Primers)

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Rtn4a-GFP was a gift from Gia Voeltz (Addgene plasmid # 61807 ; http://n2t.net/addgene:61807 ; RRID:Addgene_61807)
  • For your References section:

    The reticulon and DP1/Yop1p proteins form immobile oligomers in the tubular endoplasmic reticulum. Shibata Y, Voss C, Rist JM, Hu J, Rapoport TA, Prinz WA, Voeltz GK. J Biol Chem. 2008 Jul 4. 283(27):18892-904. 10.1074/jbc.M800986200 PubMed 18442980