mCh-Rab7A
(Plasmid #61804)

• Purpose: Human Rab7A fluorescently tagged with mCh on the N-terminus for mammalian expression
• Depositing Lab: Gia Voeltz
• Publication: Rowland et al Cell. 2014 Nov 20;159(5):1027-41. doi: 10.1016/j.cell.2014.10.023.

Backbone

• Vector backbone: pAcGFP1-C1
• Backbone manufacturer: Clontech
• Backbone size w/o insert (bp): 4700
• Total vector size (bp): 5324
• Modifications to backbone: mCh tag was amplified and inserted into the NheI and XhoI sites in order to replace the GFP.
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): XL1 Blue
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: Rab7A
• Species: H. sapiens (human)
• Insert Size (bp): 624
• Mutation: None
• GenBank ID: NM_004637.5
• Entrez Gene: RAB7A (a.k.a. CMT2B, PRO2706, RAB7)
• Promoter: CMV
• Tag / Fusion Protein:
  • mCh (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: XhoI (not destroyed)
• 3′ cloning site: HindIII (not destroyed)
• 5′ sequencing primer: CCA TCG TGG AAC AGT ACG AACG
• 3′ sequencing primer: none

Resource Information

• Articles Citing this Plasmid:
  • 15 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Takara Bio Limited Use Label License (formerly Clontech)
• Industry Terms:
  • Not Available to Industry