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Addgene

mRFP-Rab5
(Plasmid #14437)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 14437 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pmRFP-C3
  • Backbone size w/o insert (bp) 4656
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Rab 5
  • Alt name
    Rab5
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    663
  • GenBank ID
    NM_004162
  • Entrez Gene
    RAB5A (a.k.a. RAB5)
  • Tag / Fusion Protein
    • mRFP (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site HindIII (not destroyed)
  • 3′ cloning site BamHI (not destroyed)
  • 5′ sequencing primer GACTACACCATCGTGGAA
  • (Common Sequencing Primers)

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    mRFP-Rab5 was a gift from Ari Helenius (Addgene plasmid # 14437 ; http://n2t.net/addgene:14437 ; RRID:Addgene_14437)
  • For your References section:

    Rab7 associates with early endosomes to mediate sorting and transport of Semliki forest virus to late endosomes. Vonderheit A, Helenius A. PLoS Biol. 2005 Jul . 3(7):e233. 10.1371/journal.pbio.0030233 PubMed 15954801