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PurposeMammalian expression of Rab5 fused to mRFP. Used for localization to the early endosome, and other early endosomal vacuoles.
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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 14437 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepmRFP-C3
- Backbone size w/o insert (bp) 4656
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Vector typeMammalian Expression
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameRab 5
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Alt nameRab5
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SpeciesH. sapiens (human)
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Insert Size (bp)663
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GenBank IDNM_004162
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Entrez GeneRAB5A (a.k.a. RAB5)
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Tag
/ Fusion Protein
- mRFP (N terminal on backbone)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site HindIII (not destroyed)
- 3′ cloning site BamHI (not destroyed)
- 5′ sequencing primer GACTACACCATCGTGGAA (Common Sequencing Primers)
Resource Information
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Articles Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
mRFP-Rab5 was a gift from Ari Helenius (Addgene plasmid # 14437 ; http://n2t.net/addgene:14437 ; RRID:Addgene_14437) -
For your References section:
Rab7 associates with early endosomes to mediate sorting and transport of Semliki forest virus to late endosomes. Vonderheit A, Helenius A. PLoS Biol. 2005 Jul . 3(7):e233. 10.1371/journal.pbio.0030233 PubMed 15954801