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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 12605 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepEGFP-C1
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Backbone manufacturerClontech
- Backbone size w/o insert (bp) 4700
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Vector typeMammalian Expression
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameRAB7 (Homo sapiens)
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Alt nameGFP-rab7 wild type
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SpeciesH. sapiens (human)
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Insert Size (bp)693
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GenBank IDNM_004637
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Entrez GeneRAB7A (a.k.a. CMT2B, PRO2706, RAB7)
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Tags
/ Fusion Proteins
- GFP (N terminal on backbone)
- HA (N terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site BAMHI (not destroyed)
- 3′ cloning site BAMHI (not destroyed)
- 5′ sequencing primer EGFP-C
- 3′ sequencing primer SV40pA-R (Common Sequencing Primers)
Resource Information
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A portion of this plasmid was derived from a plasmid made byIMAGE Clone collection at ATCC
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Articles Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
GFP-rab7 WT was a gift from Richard Pagano (Addgene plasmid # 12605 ; http://n2t.net/addgene:12605 ; RRID:Addgene_12605) -
For your References section:
Rab proteins mediate Golgi transport of caveola-internalized glycosphingolipids and correct lipid trafficking in Niemann-Pick C cells. Choudhury A, Dominguez M, Puri V, Sharma DK, Narita K, Wheatley CL, Marks DL, Pagano RE. J Clin Invest. 2002 Jun . 109(12):1541-50. 10.1172/JCI15420 PubMed 12070301