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Plasmid#114104Purposefusion protein of Gaussia luciferase variant slow burn, Volvox channelrhodopsin-1, and enhanced yellow fluorescent protein for bioluminescent optogeneticsDepositorInsertsbGLuc-VChR1-EYFP
UseAAVTagsExpressionMammalianMutationPromoterCAGAvailable sinceAug. 9, 2019AvailabilityAcademic Institutions and Nonprofits only -
pENTR_L1-BirA(R118G)-NES-YFP-STOP-L2
Plasmid#127357Purposegateway entry vector for expressing cytosolic BirA* (R118G)-YFP under a promoter of choiceDepositorInsertBirA* (R118G mutant)
UseGateway entry vectorTagsGS linker, NES, V5, and YFPExpressionMutationR118GPromoterno promoterAvailable sinceSept. 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLenti-CaMKIIa-hChR2(C128A)-EYFP-WPRE
Plasmid#20293PurposeLentiviral expression of ChR2 (C128A) Step Function Opsin (SFO) fused to EYFP, for bi-stable optogenetic excitationDepositorInsertchannelrhodopsin-2
UseLentiviralTagsEYFPExpressionMammalianMutationchanged Cysteine 128 to AlaninePromoterAvailable sinceMay 13, 2009AvailabilityAcademic Institutions and Nonprofits only -
Myopathy Loop Mutant Nuclear Myosin I YFP
Plasmid#60243PurposeExpresses a myopathy loop mutant (6 mutations) of the nuclear isoform of myosin IC attached to YFP and an NLS, which has impaired actin binding activityDepositorInsertNuclear Myosin 1 (Myo1c Mouse)
UseTagsEYFP and NLSExpressionMammalianMutationDeleted I339, I340, G343, E344, E345,L346PromoterCMVAvailable sinceDec. 2, 2014AvailabilityAcademic Institutions and Nonprofits only -
pBS-TarHom-Tol2-kanaR-cryaa-YFP
Plasmid#74154PurposeBAC targeting cassette for pTARBAC2 with kanaR selection marker and lens-specific transgenesis marker CitrineDepositorInsertTol2-kanaR-cryaa-YFP
UseTagsExpressionMutationPromoterAvailable sinceMarch 28, 2016AvailabilityAcademic Institutions and Nonprofits only -
pAAV-Ef1a-sCreDIO hChR2(H134R)-EYFP
Plasmid#55642PurposesCre-Dependent ChR2-EYFPDepositorInserthChR2(H134R)-EYFP
UseAAV; Scre-dependentTagsEYFPExpressionMammalianMutationPromoterEf1aAvailable sinceAug. 8, 2014AvailabilityAcademic Institutions and Nonprofits only -
YFP(159-238)-gamma-1 in pcDNAI/Amp
Plasmid#54471PurposeA carboxyl-terminal YFP fragment was fused to Ggamma-1. When co-expressed with an amino-terminal YFP fragment fused to a Gbeta subunit with which it interacts, a fluorescent signal is produced.DepositorInsertYFP(159-238)-gamma-1 (GNGT1 Aequorea victoria, Bovine)
UseTagsYFP(159-238) was fused to the amino terminus of G…ExpressionMammalianMutationGgamma-1 was amplified by PCR, which added a Bam…PromoterCMVAvailable sinceJuly 30, 2015AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn-LMO4 (GLucM23-VChR1-EYFP)
Plasmid#114101Purposefusion protein of Gaussia luciferase variant M23, Volvox channelrhodopsin-1, and enhanced yellow fluorescent protein for bioluminescent optogeneticsDepositorInsertGaussia luciferase, M23; Volvox Channelrhodopsin 1; EYFP
UseAAVTagsExpressionMammalianMutationPromoterhSynAvailable sinceAug. 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1 Flag YFP hNKCC1 HA-ECL4 (NT935)
Plasmid#49065PurposeExpresses human NKCC1 mutant with HA tag inserted into extracellular loop #4 and N-terminal 3xFLAG-YFP tag in mammalian cells. cDNA is synthetic, containing convenient restriction sites.DepositorInserthuman NKCC1 (synthetic) (SLC12A2 Human, Synthetic)
UseTags3x Flag and mVenusExpressionMammalianMutation2xHA epitope in ECL4 inserted at aa574 in hNKCC1 …PromoterCMVAvailable sinceNov. 12, 2013AvailabilityAcademic Institutions and Nonprofits only -
pAAV-nEF-Coff/Fon-bREACHes-EYFP
Plasmid#137147PurposeIntersectional viral expression of bREACHes-EYFP in cells expressing Flp AND NOT CreDepositorInsertCoff/Fon-bREACHes-EYFP
UseAAV, Cre/Lox, and Synthetic Biology ; Flp/frtTagsExpressionMutationn/aPromoternEFAvailable sinceJune 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLenti-CaMKIIa-hChR2(C128T)-EYFP-WPRE
Plasmid#20295PurposeLentiviral expression of ChR2 (C128T) Step Function Opsin (SFO) fused to EYFP, for bi-stable optogenetic excitationDepositorInsertchannelrhodopsin-2
UseLentiviralTagsEYFPExpressionMammalianMutationchanged Cysteine 128 to ThreoninePromoterAvailable sinceMay 13, 2009AvailabilityAcademic Institutions and Nonprofits only -
pAAV-Ef1a-FAS-NpHR3.0-eYFP-WPRE-pA
Plasmid#37088PurposeExpresses NpHR3.0-EYFP in cells lacking Cre (Cre-Off, flanked by alternative lox FAS sites), for optogenetic inhibitionDepositorInsertNpHR3.0-EYFP
UseAAV and Cre/Lox; Cre-offTagsEYFPExpressionMutationPromoterEF1aAvailable sinceJuly 27, 2012AvailabilityAcademic Institutions and Nonprofits only -
pAAV-Ef1a-DIO-Egr3-EYFP-WPRE
Plasmid#186417PurposeOverexpression of Egr3 transcript variant 1 CDS under EF-1α promoterDepositorInsertEarly growth response 3 (Egr3 Mouse)
UseAAVTagsEYFPExpressionMutationPromoterEF-1-alphaAvailable sinceJuly 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBS-TarHom-Tol2-TetR-cryaa-YFP
Plasmid#73202PurposeBAC targeting cassette for pTARBAC2 with tetR selection marker and lens-specific transgenesis marker CitrineDepositorInsertYFP
UseTagsExpressionMutationPromoterAvailable sinceMarch 8, 2016AvailabilityAcademic Institutions and Nonprofits only -
PacrAB-acrAB-rfp + PmutS-mutS-yfp
Plasmid#121443Purposeplasmid expressing translational fusions of AcrAB and MutSDepositorInsertsPmutS-mutS-yfp
PacrAB-acrAB-rfp
UseSynthetic Biology; Pbb vectorTagsExpressionBacterialMutationPromoterPacrAB and PmutSAvailable sinceMay 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1 Flag YFP hNKCC1 HA-ECL2 (NT931)
Plasmid#49063PurposeExpresses human NKCC1 mutant with HA tag inserted into extracellular loop #2 and an N-terminal 3xFLAG-YFP tag in mammalian cells. cDNA is synthetic, containing convenient restriction sites.DepositorInserthuman NKCC1 (synthetic) (SLC12A2 Human, Synthetic)
UseTags3x Flag and mVenusExpressionMammalianMutation2xHA epitope in ECL2 inserted at aa398 in hNKCC1 …PromoterCMVAvailable sinceNov. 12, 2013AvailabilityAcademic Institutions and Nonprofits only -
YFP(159-238)-gamma-7 in pcDNAI/Amp
Plasmid#54473PurposeA carboxyl-terminal YFP fragment was fused to Ggamma-7. When co-expressed with an amino-terminal YFP fragment fused to a Gbeta subunit with which it interacts, a fluorescent signal is produced.DepositorInsertYFP(159-238)-gamma-7 (GNG7 Human, Aequorea victoria)
UseTagsYFP(159-238) was fused to the amino terminus of G…ExpressionMammalianMutationGgamma-7 was amplified by PCR, which added a BamH…PromoterCMVAvailable sinceJuly 30, 2015AvailabilityAcademic Institutions and Nonprofits only -
pTH33 [pmyo-3::YFP-BeCyclOp(A-2x)::SL2::mCherry]
Plasmid#168169PurposeExpression of YFP-BeCyclOp(A-2x) in BWMs of C. elegansDepositorInsertYFP-BeCyclOp(A-2x) (codon optimized for C. elegans)
UseTagsYFPExpressionWormMutationPromoterAvailable sinceMay 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
pRS416 Gal-RNQ1 153-405-YFP
Plasmid#18689DepositorInsertRnq1 153-405
UseTagsYFPExpressionYeastMutationPrion domain of Rnq1PromoterAvailable sinceJune 11, 2008AvailabilityAcademic Institutions and Nonprofits only -
YFP(159-238)Stop in pcDNA1/amp
Plasmid#60891PurposeThis vector attaches YFP(159-238) to the C-terminus of a protein.DepositorInsertYFP(159-238)Stop
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceJuly 30, 2015AvailabilityAcademic Institutions and Nonprofits only