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Plasmid#120269PurposeAllows for initial cellular transduction of EnvA-pseudotyped Rb via Cre-dependent expression of an avian TVA receptor fused with yellow fluorescent proteinDepositorInserteSyn-DIO-TVA950:YFP
UseAAVTagsExpressionMammalianMutationPromoterAvailable sinceMarch 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
YFP(159-238)-gamma-2 in pcDNAI/Amp
Plasmid#54472PurposeA carboxyl-terminal YFP fragment was fused to Ggamma-2. When co-expressed with an amino-terminal YFP fragment fused to a Gbeta subunit with which it interacts, a fluorescent signal is produced.DepositorInsertYFP(159-238)-gamma -2 (GNG2 Human, Aequorea victoria)
UseTagsYFP(159-238) was fused to the amino terminus of G…ExpressionMammalianMutationGgamma-2 was amplified by PCR, which added a BamH…PromoterCMVAvailable sinceJuly 30, 2015AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-CAG-LMO1 (GLuc-ChR2-EYFP)
Plasmid#72888Purposefusion protein of Gaussia luciferase, Channelrhodopsin-2, and enhanced yellow fluorescent protein for bioluminescent optogeneticsDepositorInsertGaussia luciferase, Channelrhodopsin-2, EYFP
UseTagsExpressionBacterial and MammalianMutationPromoterCAGAvailable sinceAug. 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAG306-pTet07-synYFP[TTG/AGA]-nLuc
Plasmid#199756PurposeElongation reporter construct to quantify elongation of synYFP[TTG]; Control construct of elongation reporter to quantify elongation duration of n_CGA stallingDepositorInsertsynYFP[TTG/AGA]-nLuc
UseTagsExpressionYeastMutationPromoterTetO7Available sinceMay 25, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAAV-Hb9-LMO3 (sbGLuc-VChR1-EYFP)
Plasmid#114103Purposefusion protein of Gaussia luciferase variant slow burn, Volvox channelrhodopsin-1, and enhanced yellow fluorescent protein for bioluminescent optogeneticsDepositorInsertsbGLuc-VChR1-EYFP
UseAAVTagsExpressionMammalianMutationPromotermotoneuron-specific Homeobox 9 promoterAvailable sinceAug. 9, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn-LMO3 (sbGLuc-VChR1-EYFP)
Plasmid#114099Purposefusion protein of Gaussia luciferase variant slow burn, Volvox channelrhodopsin-1, and enhanced yellow fluorescent protein for bioluminescent optogeneticsDepositorInsertGaussia luciferase, slow burn; Volvox Channelrhodopsin 1; EYFP
UseAAVTagsExpressionMammalianMutationPromoterhSynAvailable sinceAug. 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLenti-CaMKIIa-hChR2(C128S)-EYFP-WPRE
Plasmid#20294PurposeLentiviral expression of ChR2 (C128S) Step Function Opsin (SFO) fused to EYFP, for bi-stable optogenetic excitationDepositorInsertchannelrhodopsin-2
UseLentiviralTagsEYFPExpressionMammalianMutationchanged Cysteine 128 to SerinePromoterAvailable sinceMay 13, 2009AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CaMKIIa-ChRmine-eYFP-Kv2.1-WPRE
Plasmid#130989PurposeAAV vector to drive the expression of soma-targeted ChRmine-eYFP under the control of CamKIIa promoterDepositorInsertChRmine
UseAAVTagsEYFP-Kv2.1ExpressionMutationPromoterCaMKIIaAvailable sinceSept. 9, 2019AvailabilityAcademic Institutions and Nonprofits only -
RabV CVS-N2c(deltaG)-hChR2-YFP
Plasmid#73465PurposeExpresses channelrhodopsin fused to YFP for monosynaptic circuit mapping and optogenetic activationDepositorInserthChR2-YFP
UseNeurotropic virusTagsExpressionMutationPromoterAvailable sinceMarch 28, 2016AvailabilityAcademic Institutions and Nonprofits only -
pAAV-EF1α-FRT-FLEX-GtACR1-EYFP
Plasmid#114370Purposeexpresses Flpo recombinase-dependent GtACR1DepositorInsertGtACR1-EYFP (NEWENTRY )
UseAAVTagsEYFPExpressionMammalianMutationPromoterEf1aAvailable sinceAug. 24, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAAV-nEF-Con/Fon-bREACHes-EYFP
Plasmid#137145PurposeIntersectional viral expression of bREACHes-EYFP in cells expressing both Cre AND FlpDepositorInsertCon/Fon-bREACHes-EYFP
UseAAV, Cre/Lox, and Synthetic Biology ; Flp/frtTagsExpressionMutationn/aPromoternEFAvailable sinceJune 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
pEYFPC1-ECFP/GgVcl 1-419 control 1 FRET
Plasmid#46277DepositorInsertECFP + Vcl 1-419 (VCL Chicken)
UseTagsEYFPExpressionMammalianMutationaa 1-419PromoterCMVAvailable sinceAug. 5, 2013AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn-iLMO4 (GLucM23-iChloC-EYFP)
Plasmid#114102Purposefusion protein of Gaussia luciferase variant M23, improved slowChloC anion conducting ChR, and enhanced yellow fluorescent protein for bioluminescent optogeneticsDepositorInsertGLucM23-iChloC-EYFP
UseAAVTagsExpressionMammalianMutationPromoterhSynAvailable sinceAug. 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
pENTR_L1-BirA(R118G)-YFP-NLS-STOP-L2
Plasmid#127360Purposegateway entry vector for expressing nuclear BirA* (R118G)-YFP under a promoter of choiceDepositorInsertBirA* (R118G mutant)
UseGateway entry vectorTagsGS linker, NLS, V5, and YFPExpressionMutationR118GPromoterno promoterAvailable sinceSept. 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLenti-CaMKIIa-hChR2(C128A)-EYFP-WPRE
Plasmid#20293PurposeLentiviral expression of ChR2 (C128A) Step Function Opsin (SFO) fused to EYFP, for bi-stable optogenetic excitationDepositorInsertchannelrhodopsin-2
UseLentiviralTagsEYFPExpressionMammalianMutationchanged Cysteine 128 to AlaninePromoterAvailable sinceMay 13, 2009AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CAG-LMO3 (sbGLuc-VChR1-EYFP)
Plasmid#114104Purposefusion protein of Gaussia luciferase variant slow burn, Volvox channelrhodopsin-1, and enhanced yellow fluorescent protein for bioluminescent optogeneticsDepositorInsertsbGLuc-VChR1-EYFP
UseAAVTagsExpressionMammalianMutationPromoterCAGAvailable sinceAug. 9, 2019AvailabilityAcademic Institutions and Nonprofits only -
pENTR_L1-BirA(R118G)-NES-YFP-STOP-L2
Plasmid#127357Purposegateway entry vector for expressing cytosolic BirA* (R118G)-YFP under a promoter of choiceDepositorInsertBirA* (R118G mutant)
UseGateway entry vectorTagsGS linker, NES, V5, and YFPExpressionMutationR118GPromoterno promoterAvailable sinceSept. 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
Myopathy Loop Mutant Nuclear Myosin I YFP
Plasmid#60243PurposeExpresses a myopathy loop mutant (6 mutations) of the nuclear isoform of myosin IC attached to YFP and an NLS, which has impaired actin binding activityDepositorInsertNuclear Myosin 1 (Myo1c Mouse)
UseTagsEYFP and NLSExpressionMammalianMutationDeleted I339, I340, G343, E344, E345,L346PromoterCMVAvailable sinceDec. 2, 2014AvailabilityAcademic Institutions and Nonprofits only -
pBS-TarHom-Tol2-kanaR-cryaa-YFP
Plasmid#74154PurposeBAC targeting cassette for pTARBAC2 with kanaR selection marker and lens-specific transgenesis marker CitrineDepositorInsertTol2-kanaR-cryaa-YFP
UseTagsExpressionMutationPromoterAvailable sinceMarch 28, 2016AvailabilityAcademic Institutions and Nonprofits only -
pAAV-Ef1a-sCreDIO hChR2(H134R)-EYFP
Plasmid#55642PurposesCre-Dependent ChR2-EYFPDepositorInserthChR2(H134R)-EYFP
UseAAV; Scre-dependentTagsEYFPExpressionMammalianMutationPromoterEf1aAvailable sinceAug. 8, 2014AvailabilityAcademic Institutions and Nonprofits only