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Showing: 3501 - 3550 of 156588 results

  4. pLKO5.U6.DR130(CasRX)_GFP-targeting sgRNA.EFS.tRFP657

    Plasmid
    #212963
    Purpose
    Lentiviral plasmid for the measurement of RfxCas13d (CasRx) nuclease activity. As a marker, the plasmid also encodes the tRFP657 fluorescence protein.
    Depositor
    Roland Rad
    Article
    Montero et al Nat Methods. 2024 Feb 26. doi: 10.1038/s
    Insert
    DR1_CasRx_GFP targeting spacer
    Use
    CRISPR and Lentiviral
    Tags
    TagRFP657
    Expression
    Mammalian
    Mutation
    Promoter
    U6
    Available since
    Feb. 21, 2024
    Availability
    Academic Institutions and Nonprofits only
    212963-plasmid-map-sequence-id-422499

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  16. pAB1

    Plasmid
    #62547
    Purpose
    broad host range plasmid for IPTG-inducible expression of eGFP. Designed for imaging Pseudomonas aeruginosa activity in biofilms.
    Depositor
    Michael Franklin
    Article
    Walters MC et al Antimicrob Agents Chemother. 2003 Jan;47
    Insert
    eGFP
    Use
    Broad host range plasmid
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    pTrc
    Available since
    April 21, 2015
    Availability
    Academic Institutions and Nonprofits only
    62547-plasmid-map-sequence-id-314155

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  20. pHP138 Myc-TRF2-deltaB deltaM

    Plasmid
    #164255
    Purpose
    for generation of mammalian cells with inducible expression of dominant negative TRF2 for induction of telomere crisis used together with any PiggyBac transposase plasmid
    Depositor
    John Maciejowski
    Article
    Mohr et al Mol Cell. 2021 Jan 12. pii: S1097-2765(2
    Insert
    TRF2
    Use
    Tags
    Myc
    Expression
    Mammalian
    Mutation
    deletion of amino acids-86 and 496-542
    Promoter
    Tetracycline inducible promotor
    Available since
    April 13, 2021
    Availability
    Academic Institutions and Nonprofits only
    164255-plasmid-map-sequence-id-329101

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  21. TFORF3473

    Plasmid
    #144949
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    RUNX1 (RUNX1 Human)
    Use
    Lentiviral
    Tags
    V5
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Available since
    May 9, 2023
    Availability
    Academic Institutions and Nonprofits only
    144949-plasmid-map-sequence-id-392630

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  22. PB-TO-ASCL1-DLX2

    Plasmid
    #182307
    Purpose
    Piggybac Tet-ON plasmid for differentiating hiPSCs into GABAergic neuron via ASCL1 and DLX2 expression
    Depositor
    iPSC Neurodegenerative Disease Initiative (iNDI), Michael Ward
    Inserts
    Ascl1 (Ascl1 Mouse)
    Dlx2 (Dlx2 Mouse)
    puroR-T2A-mycNLS-mTagBFP2
    Use
    Tags
    None and T2A-mycNLS-mTagBFP2
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a and pTRE3G-bidirectional
    Available since
    April 14, 2022
    Availability
    Academic Institutions and Nonprofits only
    182307-plasmid-map-sequence-id-359517

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  32. pDEST17-His10-ccdB

    Plasmid
    #166145
    Purpose
    Gateway destination vector for generation of N-terminal His10-tagged proteins in E. coli.
    Depositor
    Daniel Jarosz
    Article
    Itamar Harel et al BioRxiv
    Type
    Empty backbone
    Use
    Tags
    His10
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    May 20, 2024
    Availability
    Academic Institutions and Nonprofits only
    166145-plasmid-map-sequence-id-328877

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  38. pCS2+HyPer7-NLS

    Plasmid
    #136468
    Purpose
    Mammalian expression of nucleus targeted ultrasensitive hydrogen peroxide indicator HyPer7 for optical imaging
    Depositor
    Vsevolod Belousov
    Article
    Pak et al Cell Metab. 2020 Mar 3;31(3):642-653.e6.
    Insert
    HyPer7
    Use
    Tags
    Triple nuclear localization signal of SV40 (simia…
    Expression
    Mammalian
    Mutation
    Promoter
    CMV, SP6
    Available since
    Aug. 18, 2020
    Availability
    Academic Institutions and Nonprofits only
    136468-plasmid-map-sequence-id-308715

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  47. pLGB36

    Plasmid
    #135621
    Purpose
    Suicide vector for allelic replacement in Bacteroides species including B. fragilis strain 638R, erythromycin selection and aTC-inducible ss-Bfe3 counterselection
    Depositor
    Laurie Comstock
    Article
    Ito et al mBio. 2020 Feb 4;11(1). pii: mBio.03238-
    Insert
    bfe3
    Use
    Bacteroides suicide vector with inducible counter…
    Tags
    signal sequence for periplasmic targeting
    Expression
    Mutation
    Promoter
    Bacteroides promoter regualted by TetR transcript…
    Available since
    Jan. 22, 2020
    Availability
    Academic Institutions and Nonprofits only
    135621-plasmid-map-sequence-id-267063

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  49. pBABE

    Plasmid
    #21836
    Depositor
    David Ron
    Insert
    EGFP
    Use
    Retroviral
    Tags
    farnysylation signal
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Sept. 14, 2009
    Availability
    Academic Institutions and Nonprofits only
    21836-plasmid-map-sequence-id-10029

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Showing: 3501 - 3550 of 156588 results