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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 18749 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepWZL Hygro
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Backbone manufacturerAvailable at Addgene (#18750)
- Backbone size w/o insert (bp) 5915
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Vector typeMammalian Expression, Retroviral
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Selectable markersHygromycin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameH-Ras V12
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Alt nameHRas V12
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SpeciesH. sapiens (human)
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Insert Size (bp)526
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MutationV12
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GenBank IDNM_005343
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Entrez GeneHRAS (a.k.a. C-BAS/HAS, C-H-RAS, C-HA-RAS1, CTLO, H-RASIDX, HAMSV, HRAS1, RASH1, p21ras)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site BamHI (not destroyed)
- 3′ cloning site SalI (not destroyed)
- 5′ sequencing primer pBABE 5' (Common Sequencing Primers)
Resource Information
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A portion of this plasmid was derived from a plasmid made byJay Morgenstern
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Articles Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pWZL hygro H-Ras V12 was a gift from Scott Lowe (Addgene plasmid # 18749 ; http://n2t.net/addgene:18749 ; RRID:Addgene_18749) -
For your References section:
Oncogenic ras provokes premature cell senescence associated with accumulation of p53 and p16INK4a. Serrano M, Lin AW, McCurrach ME, Beach D, Lowe SW. Cell. 1997 Mar 7. 88(5):593-602. 10.1016/S0092-8674(00)81902-9 PubMed 9054499