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Purposegreen fluorescent glucose sensor for mammalian cells
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 126206 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepcDNA3.1(-)
- Backbone size w/o insert (bp) 5400
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameGreen Glifon50
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Insert Size (bp)1700
Resource Information
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Article Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
Green Glifon50 was a gift from Tetsuya Kitaguchi (Addgene plasmid # 126206 ; http://n2t.net/addgene:126206 ; RRID:Addgene_126206) -
For your References section:
Green Fluorescent Protein-Based Glucose Indicators Report Glucose Dynamics in Living Cells. Mita M, Ito M, Harada K, Sugawara I, Ueda H, Tsuboi T, Kitaguchi T. Anal Chem. 2019 Apr 2;91(7):4821-4830. doi: 10.1021/acs.analchem.9b00447. Epub 2019 Mar 22. 10.1021/acs.analchem.9b00447 PubMed 30869867