-
Plasmid#31818DepositorInsertMEF2-dependent promoter (-307 to -242 of Nur77/NR4A1)
UseLuciferaseTagsExpressionMammalianMutationPromoterMEF2-dependent (-307 to -242 of Nur77/NR4A1)Available sinceAug. 29, 2011AvailabilityAcademic Institutions and Nonprofits only -
Clomeleon
Plasmid#203723PurposeA genetically encoded ratiometric optical indicator for chloride ions, to detect changes in intracellular chloride concentrationDepositorInsertcyan fluorescent protein linked yellow florescent protein
UseTagsExpressionBacterial and MammalianMutationCyan Fluorescent Protein, Topaz Fluorescent Prote…PromoterAvailable sinceSept. 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
BB44_pmc.DonorR5.TS
Plasmid#199223PurposeDonor plasmid with CCR5 target sites for ITPN or HMEJ knock-in at the human CCR5 safe harbour locusDepositorInsertExpression unit for mCherry - monomeric derivative of DsRed fluorescent protein (Shaner et al., 2004)
UseGene targeting donor plasmidTagsExpressionMammalianMutationPromoterHuman PGK1 gene promoterAvailable sinceApril 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLexM-SLC7A11_GFP
Plasmid#197995PurposeExpresses SLC7A11 with an N-terminal FLAG tag and C-terminal TEV-GFP-His8 tag in mammalian cellsDepositorInsertSLC7A11 (SLC7A11 Human)
UseTagsFLAG tag and TEV-GFP-His8ExpressionMammalianMutationPromoterCMVAvailable sinceMarch 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcsII-LMP7-flag
Plasmid#86773PurposeC-terminal flag-tagged protein expression in mammalian cells, lentiviral vectorDepositorInsertimmunoproteasome beta subunit 5 (LMP7) (PSMB8 Human)
UseLentiviralTagsflagExpressionMammalianMutationPromoterEF-1aAvailable sinceJan. 26, 2017AvailabilityAcademic Institutions and Nonprofits only -
pLenti_PGK_Prkaa1
Plasmid#204356PurposeLentiviral plasmid, Expresses Prkaa1 under the control of the PGK promoterDepositorInsertAMP-activated protein kinase alpha 1 (Prkaa1 Mouse)
UseLentiviralTagsExpressionMammalianMutationPromoterPGKAvailable sinceSept. 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLXI_TRC403 SMARCB1 var 2
Plasmid#111185PurposeInducible vector (V5 tagged) with SMARCB1 variant 2DepositorInsertBAF47 (SMARCB1 Human)
UseLentiviralTagsV5ExpressionMutationPromoterTREAvailable sinceMarch 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
TetO-FUW-NfiA
Plasmid#64901PurposeInducible expression of murine CDS encoding for NfiA. Toghther with TetO-FUW-Sox9 and TetO-FUW-NfiB allows reprograming into iAstro. cells.DepositorInsertNfia (Nfia Mouse)
UseLentiviralTagsExpressionMammalianMutationPromoterTetOAvailable sinceOct. 30, 2015AvailabilityAcademic Institutions and Nonprofits only -
p2attNG-H11-long
Plasmid#51545Purposep2attNG landing pad plasmid with 5 and 3kb homology arms to the human H11 locus.DepositorInsertGFP4m
UseHuman targetingTagsExpressionMammalianMutationPromoterAvailable sinceMarch 7, 2014AvailabilityAcademic Institutions and Nonprofits only -
pRvSAHS1-SAHS1-mEGFP
Plasmid#205007PurposeThe vector contains 1kbp of the upstream and downstream regions of the sahs1 gene from Ramazzottius varieornatus, with the RvSAHS1-mEGFP gene positioned in between.DepositorInsertRvSAHS1-mEGFP with 1kbp upstream and downstream of the sahs1 gene from Ramazzottius varieornatus
UseTardigrade expressionTagsExpressionMutationPromoterAvailable sinceAug. 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
DHFR-mNeon-GluA1
Plasmid#173009PurposeFor light regulated release of GluA1 from the endoplasmic reticulum. Driven by synapsin promoter. Intracellular trafficking can be better visualized with mNeon.DepositorInsertDHFR-mNeon-GluA1
UseTagsDHFR and mNeonExpressionMammalianMutationPromoterChicken beta actin (CAG)Available sinceAug. 5, 2021AvailabilityAcademic Institutions and Nonprofits only -
H516 SONIC KrasG12D-IRES-luciferase donor
Plasmid#138178PurposeCRISPR SONIC: Kras G12D luciferase donor plasmid.DepositorInsertKrasG12D (Kras Mouse)
UseNhej donorTagsExpressionMutationPromoterAvailable sinceMarch 20, 2020AvailabilityAcademic Institutions and Nonprofits only -
CCR6-Tango
Plasmid#66243PurposeExpression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocationDepositorInsertCCR6 (CCR6 Human)
UseTagsFLAGExpressionMammalianMutationPromoterCMVAvailable sinceSept. 15, 2015AvailabilityAcademic Institutions and Nonprofits only -
REEP5-EGFP
Plasmid#210363PurposeExpresses REEP5 fused with EGFP in mammalian cellsDepositorInsertREEP5 (REEP5 Human)
UseTagsEGFPExpressionMammalianMutationPromoterAvailable sinceDec. 19, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-N-Myc_1-560 USP7
Plasmid#131244PurposeMammalian expression of N-terminally Myc-tagged USP7, minus the UBL domainsDepositorInsertUbiquitin carboxyl-terminal hydrolase 7 (USP7 Human)
UseTagsMycExpressionMammalianMutationExpresses USP7 amino acids 1 - 560. This truncati…PromoterAvailable sinceDec. 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
SMC2-mAC donor (Hygro)
Plasmid#140648PurposeSMC2 tagging with mAID-CloverDepositorInsertSMC2 (SMC2 Human)
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceNov. 12, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLL3.7_hsa-miR-34a
Plasmid#25791DepositorInserthsa-miR-34a (TP53 Human)
UseLentiviral and RNAiTagsExpressionMutationPromoterAvailable sinceJuly 13, 2010AvailabilityAcademic Institutions and Nonprofits only -
pAcGFP1-N1-RvCAHS3-GS
Plasmid#192460PurposeExpression of RvCAHS3-GS_linker-AcGFP1 in mammalian cellsDepositorInsertCAHS3
UseTagsAcGFP1ExpressionMammalianMutationPromoterCMVAvailable sinceDec. 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
lenti GFAABC1D-dCas9-KRAB-MeCP2
Plasmid#194701PurposeExpresses dCas9-KRAB-MeCP2 in AstrocytesDepositorInsertGFAABC1D promoter
UseCRISPR and LentiviralTags3xFLAGExpressionMammalianMutationNonePromoterGFAABC1DAvailable sinceFeb. 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
pWaldo-GFPe-SH1446
Plasmid#112507PurposeExpresses SH1446 in E. coli with a C-terminal TEV-GFP-His(8) tag.DepositorInsertPOT family transporter
UseTagsC terminal TEV GFP octa-His affinity tagExpressionBacterialMutationPromoterT7Available sinceNov. 29, 2018AvailabilityAcademic Institutions and Nonprofits only