159,967 results

1. pAG424GPD-ccdB

   Plasmid

   #14152

   Depositor

   Susan Lindquist

   Article

   Alberti et al Yeast. 2007 Oct . 24(10):913-9.

   Type

   Empty backbone

   Use

   Gateway destination

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   March 15, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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2. pTW199b2

   Plasmid

   #136928

   Purpose

   Directed evolution of SpCas9

   Depositor

   David Liu

   Article

   Miller et al Nat Biotechnol. 2020 Apr;38(4):471-481.

   Insert

   gIII
   

   Use

   CRISPR

   Tags

   Expression

   Bacterial

   Mutation

   none

   Promoter

   Available since

   Feb. 11, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pMRX-IZ-ANXA7-mRuby3

   Plasmid

   #184893

   Purpose

   Stable expression of ANXA7-mRuby3 (Annexin A7-mRuby3) in mammalian cells

   Depositor

   Noboru Mizushima

   Article

   Yim et al FEBS Lett. 2022 Mar 11. doi: 10.1002/187

   Insert

   ANXA7 (ANXA7 Human)
   

   Use

   Tags

   mRuby3

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 13, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pU6-sgRNA-CBh-eGFP

   Plasmid

   #206885

   Purpose

   cloning backbone for sgRNA using OptScf2

   Depositor

   Jacob Giehm Mikkelsen

   Article

   Haldrup et al Nucleic Acids Res. 2023 Sep 8:gkad676. d

   Type

   Empty backbone

   Use

   Tags

   Expression

   Mutation

   Promoter

   U6

   Available since

   Oct. 20, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLenti-Puro-sgNUAK1

   Plasmid

   #138692

   Purpose

   Expresses a human NUAK1-targeting sgRNA and Cas9

   Depositor

   Reuben Shaw

   Article

   Hollstein et al Cancer Discov. 2019 Nov;9(11):1606-1627.

   Insert

   sgNUAK1 human (NUAK1 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   hU6

   Available since

   Feb. 26, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pLexM-SLC7A11_GFP

   Plasmid

   #197995

   Purpose

   Expresses SLC7A11 with an N-terminal FLAG tag and C-terminal TEV-GFP-His8 tag in mammalian cells

   Depositor

   Simon Newstead

   Article

   Parker et al Nat Commun. 2021 Dec 8;12(1):7147. doi:

   Insert

   SLC7A11 (SLC7A11 Human)
   

   Use

   Tags

   FLAG tag and TEV-GFP-His8

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   March 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. MSCV Puro-CCSP:GFP

   Plasmid

   #68487

   Purpose

   Retrovirus expressing GFP driven by CCSP promoter

   Depositor

   Tyler Jacks

   Article

   Akama-Garren et al Sci Rep. 2016 Feb 18;6:16836. doi: 10.10

   Inserts

   Puromycin Resistance
   GFP
   

   Use

   Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CCSP and LTR

   Available since

   Feb. 22, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. pcDNA3-HA-14-3-3 gamma

   Plasmid

   #13274

   Depositor

   Michael Yaffe

   Insert

   14-3-3 gamma (Ywhag Rat)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 7, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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9. pMSCV-FLAG-hIKAROS-IRES-GFP

   Plasmid

   #74046

   Purpose

   retroviral plasmid for expression of FLAG-tagged human Ikaros corresponding to cDNA from XM_011515058.1

   Depositor

   Ria Baumgrass

   Article

   Gabriel et al J Biol Chem. 2016 Sep 16. pii: jbc.M116.

   Insert

   human Ikaros (IKZF1) (IKZF1 Human)
   

   Use

   Retroviral

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   pMSCV-LTRs

   Available since

   June 8, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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10. pKRG3-mU6-PUb-hSpCas9

    Plasmid

    #162162

    Purpose

    Expresses hSpCas9 (Ae. aegypti PUb promoter) with cloning site for sgRNAs (Ae. aegypti U6 promoter)

    Depositor

    Charles Rice

    Article

    Rozen-Gagnon et al Sci Rep. 2021 Jan 12;11(1):736. doi: 10.

    Inserts

    hSpCas9
    sgRNA
    

    Use

    CRISPR

    Tags

    N/A

    Expression

    Insect

    Mutation

    Promoter

    Ae. aegypti PUb and Ae. aegypti U6

    Available since

    Jan. 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pTETRIS-cargo

    Plasmid

    #126033

    Purpose

    Luciferase reporter construct for the study of cis-repressive activity in long noncoding RNAs. Also confers puromycin resistance.

    Depositor

    Mauro Calabrese

    Article

    Kirk et al Nat Genet. 2018 Oct;50(10):1474-1482. do

    Type

    Empty backbone

    Use

    Luciferase; Piggybac

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    TRE

    Available since

    June 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pGJK_His-SUMO-LbuCas13a

    Plasmid

    #172488

    Purpose

    Bacterial expression of codon-optimized His-SUMO-LbuCas13a.

    Depositor

    Jennifer Doudna

    Article

    Liu et al Nat Chem Biol. 2021 Sep;17(9):982-988. d

    Insert

    bdSUMO-LbuCas13a (codon-optimized)
    

    Use

    Tags

    His6 and bdSUMO

    Expression

    Bacterial

    Mutation

    Promoter

    T7 promoter and lac operator

    Available since

    Aug. 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pBHt2

    Plasmid

    #104175

    Purpose

    Empty backbone for Agrobacterium tumefaciens-mediated transformation of Fusarium oxysporum

    Depositor

    Seogchan Kang

    Article

    Mullins et al Phytopathology. 2001 Feb;91(2):173-80. d

    Type

    Empty backbone

    Use

    Fungal expression

    Tags

    Expression

    Mutation

    Promoter

    none

    Available since

    Jan. 3, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. pSL1180-HR-Aag2-loxP-PUb-RFP-loxP-3xFLAG-AGO1

    Plasmid

    #162164

    Purpose

    Selectable homology donor template for Aag2 cell AGO1

    Depositor

    Charles Rice

    Article

    Rozen-Gagnon et al Sci Rep. 2021 Jan 12;11(1):736. doi: 10.

    Inserts

    RFP
    3xFLAG
    

    Use

    CRISPR and Cre/Lox; Homology donor template

    Tags

    AGO1 homology and loxP

    Expression

    Insect

    Mutation

    Promoter

    Ae. aegypti PUb and endogenous AGO1

    Available since

    Jan. 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. CMV:eGFP-p2A-HA-β2Ar-uTEV1Δ(220-242)

    Plasmid

    #135459

    Purpose

    Mammalian expression of SPARK protease component

    Depositor

    Alice Ting

    Article

    Sanchez et al Nat Methods. 2019 Dec 9. pii: 10.1038/s4

    Insert

    eGFP-p2A-HA-βarrestin2-uTEV1Δ
    

    Use

    Lentiviral

    Tags

    eGFP, HA

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 14, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. eGFP-proα1(I)

    Plasmid

    #119843

    Purpose

    Expresses mouse Type I procollagen α1 chain (Col1a1) with eGFP replacing exon 2-3 retaining N-propeptide minor triple helix and N-terminal cleavage site

    Depositor

    Sergey Leikin

    Article

    Omari et al Proc Natl Acad Sci U S A. 2018 Oct 23;11

    Insert

    Type I procollagen α1 chain (Col1a1 Mouse)
    

    Use

    Tags

    eGFP

    Expression

    Mammalian

    Mutation

    Col1a1 exons 2-3 replaced by fluorescent tag

    Promoter

    CMV

    Available since

    April 2, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. pcDNA3-mNG-IRES-mScarlet-dPCP

    Plasmid

    #231090

    Purpose

    Bicistronic construct encoding mNG in combination with mScarlet-dPCP via an EMCV-IRES sequence.

    Depositor

    John Ngo

    Insert

    mNG-IRES-mScarlet-dPCP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 17, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCMV-PL

    Plasmid

    #20783

    Depositor

    Bryan Cullen

    Article

    Zeng et al Mol Cell. 2002 Jun . 9(6):1327-33.

    Type

    Empty backbone

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 5, 2009

    Availability

    Academic Institutions and Nonprofits only

19. LentiCRISPR v2 hBAK

    Plasmid

    #129579

    Purpose

    CRISPR deletion of human BAK

    Depositor

    Stephen Tait

    Article

    Lopez et al Nat Commun. 2016 Feb 2;7:10538. doi: 10.

    Insert

    gRNA vs human BAK (BAK1 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    None

    Promoter

    Available since

    Aug. 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. Prab-3::NLS::GFP

    Plasmid

    #68120

    Purpose

    Nuclear Localized GFP for pan-neuronal expression in C. Elegans

    Depositor

    Andrew Leifer

    Article

    Nguyen et al Proc Natl Acad Sci U S A. 2015 Dec 28. p

    Insert

    GFP
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Promoter

    rab-3

    Available since

    Aug. 18, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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