159,967 results

1. AiP12858 - pAAV-AiE0675m-minBG-SYFP2-WPRE3-BGHpA (Alias: CN2858)

   Plasmid

   #224014

   Purpose

   AiE0675m is an enhancer sequence, designed to drive AAV-mediated transgene expression in cortical L2/3_IT neurons

   Depositor

   The Allen Institute for Brain Science, Jonathan Ting

   Article

   Ben-Simon et al Cell. 2025 May 16:S0092-8674(25)00513-6.

   Insert

   SYFP2
   

   Use

   AAV

   Tags

   Expression

   Mutation

   NA

   Promoter

   minBG

   Available since

   Oct. 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pHR SFFVp BFP-Erk D319N

   Plasmid

   #102350

   Purpose

   Lentiviral expressing translational fusion between blue fluorescent protein and Erk2 bearing the D319N mutation

   Depositor

   Jared Toettcher

   Article

   Wilson et al Mol Cell. 2017 Sep 7;67(5):757-769.e5. d

   Insert

   Erk2 (Mapk1 Mouse)
   

   Use

   Lentiviral

   Tags

   BFP

   Expression

   Mutation

   Aspartic acid 319 was mutated to asparagine

   Promoter

   SFFV

   Available since

   Oct. 25, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. p3E-csGBP

   Plasmid

   #108891

   Purpose

   Multisite gateway vector for 3' tagging with a conditionally stable nanobody against GFP

   Depositor

   Rob Parton

   Article

   Ariotti et al PLoS Biol. 2018 Apr 5;16(4):e2005473. do

   Insert

   csGBP
   

   Use

   Multisite gateway

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Jan. 9, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAG306GPD-ccdB

   Plasmid

   #14140

   Depositor

   Susan Lindquist

   Article

   Alberti et al Yeast. 2007 Oct . 24(10):913-9.

   Type

   Empty backbone

   Use

   Gateway destination

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   March 16, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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5. pME-mKate2-P2A-TurboID-NS

   Plasmid

   #166568

   Purpose

   Multisite Gateway middle entry clone encoding TurboID with mKate2 marker. Parton lab clone IYN

   Depositor

   Rob Parton

   Article

   Xiong et al Elife. 2021 Feb 16;10. pii: 64631. doi:

   Insert

   mKate2-P2A-TurboID
   

   Use

   Multisite gateway entry clone

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Sept. 13, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pSG66-KcsA-Kv1.3-sfGFP

   Plasmid

   #102457

   Purpose

   Expresses a fusion protein of KcsA-Kv1.3 and sfGFP with a C-terminal His tag. The expression is controlled by a OR2-OR1-Pr promoter.

   Depositor

   Richard Murray

   Article

   Guo et al bioRxiv 104455

   Insert

   KcsA-Kv1.3
   

   Use

   Tags

   His tag and sfGFP

   Expression

   Bacterial

   Mutation

   Promoter

   OR2-OR1-Pr

   Available since

   Oct. 31, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. Mouse Ism1 with C-terminal Myc-6X-his tag

   Plasmid

   #173046

   Purpose

   Expresses Ism1 in mammalian cells

   Depositor

   Katrin Svensson

   Article

   Jiang et al Cell Metab. 2021 Jul 28. pii: S1550-4131

   Insert

   Ism1 (Ism1 Mouse)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 1, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pPB-PNGT3-d2EGFP_CMV-mCherry

   Plasmid

   #213765

   Purpose

   Fluorescent reporter for genetic tracing of mesenchymal Glioblastoma cell-state.

   Depositor

   Gaetano Gargiulo

   Article

   Company et al Nat Commun. 2024 Feb 5;15(1):897. doi: 1

   Insert

   PNGT#3-d2EGFP
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Feb. 5, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pcDNA3.1-Flag HBx (G124L, I127A)

   Plasmid

   #42598

   Depositor

   Ding Xue

   Article

   Geng et al Proc Natl Acad Sci U S A. 2012 Nov 6;109

   Insert

   Hepatitis B virus protein X (G124L, I127A)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   G124L I127A

   Promoter

   Available since

   March 22, 2013

   Availability

   Academic Institutions and Nonprofits only

10. haA1.pBABEpu

    Plasmid

    #21813

    Depositor

    David Ron

    Article

    Novoa et al J Cell Biol. 2001 May 28. 153(5):1011-22

    Insert

    GADD34 C-term
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 10, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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11. pUS116

    Plasmid

    #84578

    Purpose

    Shuttle and expression vector. Replicates in E.coli and Mycobacterium. Gives acetamide-inducible gene expression in Mycobacterium.

    Depositor

    Nicholas Coleman

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Jan. 17, 2017

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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12. pIG-874_HA-GD2-28z_CAR_IL2RA

    Plasmid

    #207504

    Purpose

    This plasmid can be used to generate HDR templates for non-viral knockin into the TRAC locus of human T cells.

    Depositor

    Alexander Marson

    Article

    Blaeschke et al Cell. 2023 Sep 14;186(19):4216-4234.e33.

    Insert

    IL2RA, HA-GD2-28z_CAR (IL2RA Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pLenti-STAT3-linker-TagGFP2

    Plasmid

    #121427

    Purpose

    WT STAT3 inserted using Infusion Cloning into MluI and EcoRI digested pLenti-Origene-Nrf21.

    Depositor

    Stanley Qi, Ovijit Chaudhuri

    Article

    Lee et al Nat Commun. 2019 Apr 23;10(1):1848. doi:

    Insert

    STAT3-linker-TagGFP2
    

    Use

    Tags

    TagGFP2

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 22, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. pETM33_AP2B1_adaptin

    Plasmid

    #178467

    Purpose

    Bacterial expression of human domain with His-tag and GST tag

    Depositor

    Ylva Ivarsson

    Article

    Benz et al Mol Syst Biol. 2022 Jan;18(1):e10584. do

    Insert

    AP2B1_adaptin (AP2B1 Synthetic, Human)
    

    Use

    Tags

    His-GST

    Expression

    Bacterial

    Mutation

    Promoter

    T7/LacO

    Available since

    March 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pFGC5941-PacI-ath-STTM156/157

    Plasmid

    #84083

    Purpose

    target miRNA156/157 for destruction in Arabidopsis thaliana

    Depositor

    Guiliang Tang

    Article

    Tang et al Methods. 2012 Oct;58(2):118-25. doi: 10.

    Insert

    STTM156/157
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    2x35S

    Available since

    Jan. 4, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. pVC_CRISPR_Cat

    Plasmid

    #120368

    Purpose

    Reporter plasmid for full-site selection assay

    Depositor

    Jennifer Doudna

    Article

    Wright et al Mol Cell. 2019 Feb 21;73(4):727-737.e3.

    Insert

    CRISPR_Cat
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 12, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. Col18a1_pCSdest

    Plasmid

    #53930

    Depositor

    Roger Reeves

    Article

    Edie et al G3 (Bethesda). 2018 Jul 2;8(7):2215-2223

    Insert

    Col18a1 (Col18a1 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    SP6 and CMV

    Available since

    March 27, 2018

    Availability

    Industry, Academic Institutions, and Nonprofits

18. pFGC5941-PacI-ath-STTM160

    Plasmid

    #84085

    Purpose

    target miRNA160 for destruction in Arabidopsis thaliana

    Depositor

    Guiliang Tang

    Article

    Tang et al Methods. 2012 Oct;58(2):118-25. doi: 10.

    Insert

    STTM160
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    2x35S

    Available since

    March 21, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. pUb-Cas9-mCherry_cU6:6-sgRNA

    Plasmid

    #190598

    Purpose

    The plasmid encodes S. pyogenes Cas9 and mCherry separated by a T2A peptide under an Aedes aegypti polyubiquitin promoter. It also expresses a sgRNA scaffold under a Culex quinquefasciatus U6 promoter

    Depositor

    Claudia Rueckert

    Article

    Torres et al Insects. 2022 Sep 19;13(9):856. doi: 10.

    Inserts

    Cas9
    sgRNA
    

    Use

    CRISPR

    Tags

    mCherry - separated by T2A

    Expression

    Mutation

    Promoter

    Aedes aegypti polyubiquitin and Culex quinquefasc…

    Available since

    Jan. 17, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. C278

    Plasmid

    #174356

    Purpose

    For introducing multiple copies of OsTIR1 with the piggyBac system

    Depositor

    David Bartel

    Article

    Xiang et al Elife. 2021 Jul 2;10. pii: 66493. doi: 1

    Insert

    OsTIR1
    

    Use

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 4, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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