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Showing: 1501 - 1520 of 155550 results

  2. pGEM4Z-T7-5'UTR-NanoLuc-3'UTR-A64

    Plasmid
    #203350
    Purpose
    Template for T7 in vitro transcription of NanoLuc mRNA flanked by Xenopus beta globin UTRs and 64nt polyA tail
    Depositor
    Christopher Grigsby, Molly Stevens
    Insert
    Nano Luciferase
    Use
    Template for t7-mediated in vitro transcription o…
    Tags
    Flanked by 5' and 3' UTRs from Xenopus …
    Expression
    Mutation
    Promoter
    T7
    Available since
    June 29, 2023
    Availability
    Academic Institutions and Nonprofits only
    203350-plasmid-map-sequence-id-399148

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  5. pSLIK-Hygro

    Plasmid
    #25737
    Purpose
    3rd generation Lentiviral vector for Tet-based inducible shRNA or cDNA expression, constitutive Hygromycin resistance gene coexpression.
    Depositor
    Iain Fraser
    Article
    Shin et al Proc Natl Acad Sci U S A. 2006 Sep 12. 1
    Type
    Empty backbone
    Use
    Lentiviral and RNAi
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Aug. 13, 2010
    Availability
    Academic Institutions and Nonprofits only
    25737-plasmid-map-sequence-id-202030

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  6. Go1-CASE

    Plasmid
    #168123
    Purpose
    Encodes a BRET-based activity sensor for the heterotrimeric G protein Go1. Composed of the subunits G alpha o1 (GNAO1) tagged with NanoLuciferase, G beta 3 (GNB3) and Venus-tagged G gamma 9 (GNG9).
    Depositor
    Gunnar Schulte
    Article
    Schihada et al Sci Signal. 2021 Sep 7;14(699):eabf1653.
    Insert
    GNAO1, GNB3, GNG9 (GNAO1, GNB3, GNGT2 Human)
    Use
    Luciferase
    Tags
    cpVenus on GNG9
    Expression
    Mammalian
    Mutation
    NLuc is inserted at L91/G92 within GNAO1
    Promoter
    Available since
    July 29, 2021
    Availability
    Academic Institutions and Nonprofits only
    168123-plasmid-map-sequence-id-332865

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  7. pPP085

    Plasmid
    #158795
    Purpose
    pRSF-Duet1 derived plasmid containing C-terminally hexa-histidine tagged CasΦ-2 in MCS1 for expression in E. coli and protein purification
    Depositor
    Jennifer Doudna
    Article
    Pausch et al Science. 2020 Jul 17;369(6501):333-337.
    Insert
    CasPhi-2
    Use
    Tags
    6xHis
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Aug. 25, 2020
    Availability
    Academic Institutions and Nonprofits only
    158795-plasmid-map-sequence-id-309510

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  13. pFastBac1-His10FLAG

    Plasmid
    #157736
    Purpose
    empty pFastBac1 vector (polyhedrin promoter) adds enterokinase-removable N-terminal His10-tag + 3xFLAG tag; avoids unwanted residues in the final protein sequence by using the BseRI restriction sites
    Depositor
    Debra Hansen
    Article
    Olson et al Protein Expr Purif. 2021 May 7:105890. d
    Type
    Empty backbone
    Use
    Tags
    His10 + 3xFLAG, enterokinase-cleavable
    Expression
    Insect
    Mutation
    Promoter
    polyhedrin
    Available since
    June 2, 2021
    Availability
    Academic Institutions and Nonprofits only
    157736-plasmid-map-sequence-id-333926

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  17. mCherry-Sequestosome1-N-18

    Plasmid
    #55132
    Purpose
    Localization: Membrane Scaffold, Excitation: 587, Emission: 610
    Depositor
    Michael Davidson
    Insert
    Sequestosome1 (SQSTM1 Human)
    Use
    Tags
    mCherry
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Aug. 29, 2014
    Availability
    Academic Institutions and Nonprofits only

  19. pmCherry-C1 R62D actin-3XNLS P2A mCherry

    Plasmid
    #58477
    Purpose
    Expresses nuclear-targeted non-polymerizing R62D mutant of human actin, with an mCherry expression reporter after a P2A protease cleavage site, on a CMV promoter
    Depositor
    Dyche Mullins
    Article
    Belin et al Elife. 2015 Aug 19;4. doi: 10.7554/eLife
    Inserts
    beta actin (ACTB Human)
    3XNLS
    P2A cleavage site
    Use
    Tags
    mCherry
    Expression
    Mammalian
    Mutation
    Changed Arginine 62 to Aspartic Acid
    Promoter
    CMV
    Available since
    Sept. 18, 2015
    Availability
    Academic Institutions and Nonprofits only
    58477-plasmid-map-sequence-id-90386

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Showing: 1501 - 1520 of 155550 results