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Plasmid#85121PurposePkaiB-kaiC-ecfp expressed from NSII-Gm vector constructed by seamless assembly. Fusion expressed as full length fusion protein at WT levels but is 5hr long period. Used only for co-localization expDepositorInsertPkaiB-KaiC-ECFP
UseTagsECFPExpressionBacterialMutationPromoterAvailable sinceMay 31, 2017AvailabilityAcademic Institutions and Nonprofits only -
pWZL Hygro-NFLAG TRF1 deltaA
Plasmid#16278DepositorInserthTRF1 (TERF1 Human)
UseRetroviralTagsFlagExpressionMammalianMutationDeleted acidic domain (aa1-66)PromoterAvailable sinceMay 14, 2008AvailabilityAcademic Institutions and Nonprofits only -
pWZL Hygro-NFLAG TRF1 deltaM
Plasmid#16281DepositorInserthTRF1 (TERF1 Human)
UseRetroviralTagsFlagExpressionMammalianMutationDeleted myb domain (aa379-439)PromoterAvailable sinceMay 14, 2008AvailabilityAcademic Institutions and Nonprofits only -
pWZL Hygro-NFLAG TRF1 deltaA deltaM
Plasmid#16297DepositorInserthTRF1 (TERF1 Human)
UseRetroviralTagsFlagExpressionMammalianMutationDeleted acidic domain (aa1-66) and myb domain (aa…PromoterAvailable sinceMay 14, 2008AvailabilityAcademic Institutions and Nonprofits only -
AAVS1-TRE-U2AF2-TurboID-HA-rtTA
Plasmid#226997PurposeIntegrative plasmid to express U2AF2-TurboID in mammalian cellsDepositorInsertU2 small nuclear RNA auxiliary factor 2 (U2AF2 Human)
UseTagsTurboID-HAExpressionMammalianMutationPromoterAvailable sinceNov. 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLenti-Cre-MS2_UbC-GFP (Cre_GFP)
Plasmid#219536Purposea. Expresses Cre recombinase tagged with 21x repeats of MS2 binding sequence under EF1A promotor and b. GFP reporter under UbC promotorDepositorInsertsUseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceNov. 11, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
AiP12360 - pAAV-AiE0519h-minBG-SYFP2-WPRE3-BGHpA (Alias: CN2360)
Plasmid#223986PurposeAiE0519h is an enhancer sequence, designed to drive AAV-mediated transgene expression in cortical Sst+ neuronsInsertSYFP2
UseAAVTagsExpressionMutationNAPromoterminBGAvailable sinceOct. 30, 2024AvailabilityAcademic Institutions and Nonprofits only -
pX552-EF1a-DIO-mcherry(with gRNA scaffold)
Plasmid#199581PurposeExpresses mCherry in a Cre-dependent mannerDepositorInsertmCherry
UseAAV, CRISPR, and Cre/LoxTagsExpressionMammalianMutationPromoterEF1a intron A and U6Available sinceOct. 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
AiP13563 - pAAV-AiE0170h_3xC2-minBG-SYFP2-WPRE3-BGHpA (Alias: CN3563)
Plasmid#224029PurposeAiE0170h_3xC2 is an enhancer sequence, designed to drive AAV-mediated transgene expression in cortical Sst+ neuronsInsertSYFP2
UseAAVTagsExpressionMutationNAPromoterminBGAvailable sinceOct. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
AiP13583 - pAAV-AiE0475m_3xC1-minBG-SYFP2-WPRE3-BGHpA (Alias: CN3583)
Plasmid#224030PurposeAiE0475m_3xC1 is an enhancer sequence, designed to drive AAV-mediated transgene expression in cortical Pvalb+ neurons (sparse ChC cells)InsertSYFP2
UseAAVTagsExpressionMutationNAPromoterminBGAvailable sinceOct. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
AiP13584 - pAAV-AiE0475m_3xC2-minBG-SYFP2-WPRE3-BGHpA (Alias: CN3584)
Plasmid#224031PurposeAiE0475m_3xC2 is an enhancer sequence, designed to drive AAV-mediated transgene expression in cortical Chandelier cellsInsertSYFP2
UseAAVTagsExpressionMutationNAPromoterminBGAvailable sinceOct. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pYX-C-mScarlet-Zeo-TSC22D2
Plasmid#222670Purposeendogenously tag TSC22D2 with C-terminal mScarletDepositorInsertTSC22D2 (TSC22D2 Human)
UseTagsmScarletExpressionBacterialMutationPromoterAvailable sinceOct. 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
pYX-N-Puro-mNeon-TSC22D4
Plasmid#222671Purposeendogenously tag TSC22D4 with N-terminal mNeonDepositorInsertTSC22D4 (TSC22D4 Human)
UseTagsmNeonExpressionBacterialMutationPromoterAvailable sinceOct. 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
prhaBAD-pA-Hia5
Plasmid#222303PurposePlasmid for rhamnose inducible expression and purification of protein A-Hia5-6XHis (pA-Hia5) for antibody directed DNA (adenine) methylation (as in DiMeLo-seq)DepositorInsertpA-Hia5
UseTags6His and protein AExpressionBacterialMutationcodon optimized for bacterial expressionPromoterrhaBADAvailable sinceOct. 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
p5E-TetO8x-CMVmin (JDW 1087)
Plasmid#224475PurposeGateway compatible 5' entry clone containing 8 tet operons (TREs) upstream of a minimal CMV promoterDepositorInsertTetO(8x)-CMV promoter
UseGateway cloningTagsExpressionMutationPromoterAvailable sinceOct. 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-link-ymScarletI-SpHis5
Plasmid#219844Purposetagging vector to tag genes with ymScarletI and a HIS5 geneDepositorInsertymScarletI-SpHis5
UseTagsExpressionYeastMutationPromoterAvailable sinceAug. 27, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
pMMS22L-siR3
Plasmid#221013Purposemammalian expression vector of myc-Flag tagged MMS22L siRNA resistantDepositorInsertMMS22L (MMS22L Human)
UseTagsFlag and mycExpressionMammalianMutationsilent mutations MMS22L: 5'-aaAacGtgTtgCtgTg…PromoterCMVAvailable sinceJuly 31, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
pYAL_HLA-A2_MART1
Plasmid#171177PurposeDisplays MART-1 peptide in HLA-A*02 as a single-chain trimerDepositorInsertMART1_HLA-A2*01 single-chain trimer fusion
UseTagsExpressionBacterial and YeastMutationPromoterGAL1Available sinceJuly 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
mAIRN HO
Plasmid#207411PurposeMammalian-cell-based plasmid system that expresses mAIRN (an R-loop-forming transcript) upon dox-induction, which will further cause head-ON transcription-replication conflict (HO TRC).DepositorInsertmAIRN
UseTagsExpressionBacterial and MammalianMutationPromoterAvailable sinceMarch 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
mAIRN CD
Plasmid#207412PurposeMammalian-cell-based plasmid system that expresses mAIRN (an R-loop-forming transcript) upon dox-induction, which will further cause co-directional transcription-replication conflict (CD TRC).DepositorInsertmAIRN
UseTagsExpressionBacterial and MammalianMutationPromoterAvailable sinceMarch 27, 2024AvailabilityAcademic Institutions and Nonprofits only