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Plasmid#189772PurposeConstruct for labelling endoplasmic reticulum in mammalian cells using ER targeted mScarlet3DepositorInsertER-mScarlet3
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceMarch 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
pEZT-BM
Plasmid#74099PurposeMulti-purpose rapid titer bacmam expression vectorDepositorHas ServiceCloning Grade DNATypeEmpty backboneUseBacmam, baculovirusTagsEGFP following p10 promoterExpressionMammalianMutationPromoterCMV, p10Available sinceMarch 28, 2016AvailabilityAcademic Institutions and Nonprofits only -
pLX303-ZIM3-KRAB-dCas9
Plasmid#154472PurposeExpresses ZIM3 KRAB domain fused to the N terminus of dCas9DepositorInsertZIM3 (ZIM3 Synthetic)
UseLentiviralTagsHAExpressionMammalianMutationIncludes ZIM3 aa 1-100PromoterAvailable sinceOct. 5, 2020AvailabilityAcademic Institutions and Nonprofits only -
pHR-flag-Lag16-iCAM-1
Plasmid#205195PurposeThis vector encodes of the synCAM (iCAM1) and GFP nanobody LAG16DepositorInsertsynCAM iCAM1 and Lag16 anti-GFP nanobody
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceAug. 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
lenti sgRNA(MS2)_zeo backbone
Plasmid#61427Purpose3rd generation lenti sgRNA cloning backbone with MS2 loops at tetraloop and stemloop 2 and EF1a-zeo resistance marker. Contains BsmBI sites for insertion of spacer sequences.DepositorHas ServiceCloning Grade DNATypeEmpty backboneUseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterU6 and EF1AAvailable sinceDec. 15, 2014AvailabilityAcademic Institutions and Nonprofits only -
pGEXTK-Pak1 70-117
Plasmid#12217DepositorInsertPak1 (PAK1 Human)
UseTagsGSTExpressionBacterialMutationPBD (p21-binding domain), aa 70-117PromoterAvailable sinceAug. 11, 2006AvailabilityAcademic Institutions and Nonprofits only -
pCMV-MMLVgag-3xNES-ABE8e-NG
Plasmid#181754PurposeMMLV-Gag fused to 3xNES-ABE8e-NG with TSTLLMENSS cleavage site between Gag-NES and ABE. For production of virus like particles with base-editor RNP cargo.DepositorInsertMMLVgag-3xNES-ABE8e-NG
UseTagsFlagExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLV-EGFP-Cre
Plasmid#86805PurposeLentiviral vector that expresses an EGFP-Cre fusion protein with a nuclear localization signal from the CMV promoterDepositorInsertCre recombinase
UseCre/Lox and LentiviralTagsEGFP and nuclear localization signal: PKKKRKVExpressionMammalianMutationPromoterCMVAvailable sinceMarch 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
psfaRb-Tn5
Plasmid#183638PurposeExpresses anti-rabbit nanobody fused with Tn5DepositorInsertanti rabbit secondary nanobody fused to Tn5
UseTagsExpressionBacterialMutationPromoterAvailable sinceJune 23, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCS2FA-H2B-mScarlet3
Plasmid#224436PurposeConstruct for labelling cell nuclei in mammalian cells using Histon protein 2B fused to mScarlet3DepositorInsertH2B-mScarlet3
UseTagsExpressionMammalianMutationPromoterCMV & SP6Available sinceSept. 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCI-Caspase 1
Plasmid#41552DepositorInsertCaspase-1 (CASP1 Human)
UseTagsMycExpressionMammalianMutationPromoterCMV immediate-early enhancer/promoterAvailable sinceDec. 20, 2012AvailabilityAcademic Institutions and Nonprofits only -
pC0037
Plasmid#181934PurposeDual secreted luciferase reporter (EF1alpha-Gaussia luciferase, CMV-Cypridina luciferase)DepositorInsertsGaussia luciferase
Cypridina luciferase
UseTagsExpressionMammalianMutationPromoterCMV and EF1alphaAvailable sinceAug. 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLX304-DCK*-IRES-GFP
Plasmid#176297PurposeExpresses DCK* and GFP from the same transcriptDepositorInsertMutant Deoxycytidine Kinase (DCK*, S74E R104M D133A) (DCK Human)
UseLentiviralTagsV5ExpressionMammalianMutationS74E R104M D133APromoterCMVAvailable sinceJuly 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
PB-SAM
Plasmid#102559PurposePiggybac transposon vector encoding dCAS9-VP64 and MS2-P65-HSF1 activator helper complex.DepositorInsertsMS2-P65-HSF1_T2A_Hygro
dCAS9(D10A, N863A)-VP64_T2A_Blast
UseCRISPR; Piggybac transposonTagsExpressionMammalianMutationD10A and N863A in Cas9 and N55K in MS2PromoterCAG and EF1AAvailable sinceJan. 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCBh_NLS_hfCas13X(Cas13X_M17YY)_NLS-pA-U6-DR-BpiI-BpiI-DR-pSV40-EGFP-pA-pSV40-mCherry-pA
Plasmid#190033Purposevector for encoding a human codon-optimized High-fidelity Cas13X (hfCas13X) driven by CBh promoter, guide RNAs compatible with Cas13X driven by hU6, EGFP and mCherry driven by SV40 promotersDepositorInserthumanized hfCas13X
UseTagsExpressionMammalianMutationY672A,Y676APromoterCBh, SV40, hU6Available sinceSept. 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
lenti sgRNA(MS2)_puro backbone
Plasmid#73795Purpose3rd generation lenti sgRNA cloning backbone with MS2 loops at tetraloop and stemloop 2 and EF1a-puro resistance marker. Contains BsmBI sites for insertion of spacer sequences.DepositorHas ServiceCloning Grade DNATypeEmpty backboneUseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterU6 and EF1AAvailable sinceMarch 15, 2016AvailabilityAcademic Institutions and Nonprofits only -
pGL3-NFAT luciferase
Plasmid#17870Purpose3x NFAT binding sequence upstream of a luciferase reporter; can be used to assay signal transduction through Ca2+, calcineurin and NFAT in any tissueDepositorInsert3x NFAT binding sequence (IL2 )
UseLuciferaseTagsIL2 promoter and LuciferaseExpressionMammalianMutationPromoterIL-2 promoterAvailable sinceApril 29, 2008AvailabilityAcademic Institutions and Nonprofits only -
pVDM1001
Plasmid#134660PurposeCRISPR delivery and repair template delivery vectorDepositorInsertCRISPR guide RNA array
UseTagsExpressionBacterialMutationPromoterAvailable sinceApril 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
pB-CAGGS-dCas9-KRAB-MeCP2
Plasmid#110824PurposePiggyBac compatible plasmid expressing dCas9-KRAB-MeCP2DepositorInsertdCas9-KRAB-MeCP2
UseTagsExpressionMammalianMutationCas9 mutations to make dCas9=D10A+D839A+H840A+N86…PromoterCAGGSAvailable sinceJuly 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
pHIV-Luc-ZsGreen
Plasmid#39196DepositorInsertsFirefly Luciferase Luc2P
ZsGreen
UseLentiviralTagsExpressionMammalianMutationPromoterEF1-alpha and EF1-alphaAvailable sinceOct. 30, 2012AvailabilityAcademic Institutions and Nonprofits only -
pENN236_CRISPRcharm_Kv2
Plasmid#220839PurposeCRISPRcharm Kv2 expression in mammalian cellsDepositorInsertCRISPRcharm Kv2
UseCRISPRTagsP2A-TagBFPExpressionMammalianMutationN/APromoterCAGAvailable sinceJune 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
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pCS2+IMS-HyPer7
Plasmid#136469PurposeMammalian expression of mitochondrial intermembrane space targeted ultrasensitive hydrogen peroxide indicator HyPer7 for optical imagingDepositorInsertHyPer7
UseTagsSMAC/DIABLOExpressionMammalianMutationPromoterCMV, SP6Available sinceFeb. 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCDH-TfR-mApple
Plasmid#179384PurposeLentiviral vector for expression of Human Transferrin receptor-1 fused mAppleDepositorInsertTransferrin receptor 1 - mApple (TFRC Human, Synthetic)
UseLentiviralTagsmAppleExpressionMammalianMutationPromoterEF1Available sinceOct. 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
6xHis-SNAP-PI Probe
Plasmid#211507PurposeFor expression of recombinant biosensor for detection of Phosphatidylinositol (PI) from bacteriaDepositorInsertBcPI-PLC-ANH
UseTags6xhis-SNAPExpressionBacterialMutationPromoterAvailable sinceJan. 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCS2+mNeonGreen-C Cloning Vector
Plasmid#128144PurposeTo express a protein of interest fused to the C-terminus of mNeonGreenDepositorTypeEmpty backboneUseTagsmNeonGreen fusionExpressionMammalianMutationPromoterAvailable sinceJuly 2, 2019AvailabilityAcademic Institutions and Nonprofits only -
pGL3 Control Vector
Plasmid#212937PurposeControl vector with constitutive firefly luciferase expressionDepositorInsertluc+
UseLuciferaseTagsExpressionMutationWTPromoterSV40Available sinceJan. 16, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
tetO.Nfia.Blast
Plasmid#117270PurposeExpresses Nfia and blasticidin resistance gene under control of tetO sequence.DepositorInsertNfia (Nfia Mouse)
UseLentiviralTagsExpressionMammalianMutationPromotertet-onAvailable sinceNov. 1, 2018AvailabilityAcademic Institutions and Nonprofits only -
pTubulin-miRFP670nano3
Plasmid#184666PurposeHuman alpha-tubulin labelled with near-infrared fluorescent protein miRFP670nano3DepositorInsertmiRFP670nano3-human alpha-tubulin
UseTagsmiRFP670nano3ExpressionMammalianMutationPromoterCMVAvailable sinceJune 23, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3/Myc-DNMT1
Plasmid#36939PurposeMammalian expression of human DNMT1 with myc tagDepositorInsertDNMT1 (DNMT1 Human)
UseTagsMycExpressionMammalianMutationPromoterCMVAvailable sinceJune 22, 2012AvailabilityAcademic Institutions and Nonprofits only -
lentiCRISPR v2-Blast
Plasmid#83480PurposeMammalian expression of Cas9 and sgRNA scaffoldDepositorInsertBlasticidin S deaminase
UseCRISPR and LentiviralTagsExpressionMammalianMutationReplaced puromycin N-acetyltransferase on the ori…PromoterEF-1αAvailable sinceNov. 10, 2016AvailabilityAcademic Institutions and Nonprofits only -
pU6-sp-sgRNA-RNF2_+41nick
Plasmid#135958PurposeS. pyogenes sgRNA for +41 nick in RNF2DepositorInsertRNF2_+41 nicking sgRNA
UseTagsExpressionMammalianMutationSee manuscriptPromoterU6Available sinceJan. 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
pFC330
Plasmid#87842PurposeAMA1 plasmid with Aspergillus optimized Cas9 and pyrG selection markerDepositorInsertsCas9
pyrG
UseCRISPR; Fungal expressionTagsSV40 NLSExpressionBacterialMutationPromoterAspergillus nidulans tef1 promoter and native pro…Available sinceJuly 28, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn-DIO-mCherry
Plasmid#50459PurposeDouble floxed mCherry under the control of human synapsin promoterDepositorHas ServiceAAV Retrograde, AAV1, AAV2, AAV5, AAV8, and AAV9InsertmCherry
UseAAVTagsN/AExpressionMutationPromoterhuman Synapsin 1Available sinceMarch 17, 2014AvailabilityAcademic Institutions and Nonprofits only -
UBQ:RUBY
Plasmid#160909PurposeRUBY under the control of Ubiquitin promoter/marker for transformationDepositorInsertRUBY and UBIQUITIN promoter
UseTagsExpressionPlantMutationPromoterUBIQUITINAvailable sinceNov. 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
pENTR4_G3BP2
Plasmid#127105DepositorInsertG3BP2 (G3BP2 Human)
UseGateway shuttling vectorTagsExpressionMutationPromoterAvailable sinceJuly 25, 2019AvailabilityAcademic Institutions and Nonprofits only -
CMV-Mito4x-GCaMP6f
Plasmid#127870PurposeFluorescent reporter for mitochondrial matrix Ca2+DepositorInsert4 repeats of COX8 signal peptide plus GCaMP6f
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceNov. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
pDestTol2CG2-U6:gRNA
Plasmid#63156PurposeDestination vector for Gateway based on vector #395 from the Tol2Kit, containing a gRNA scaffold under the control of a zebrafish U6 promoter, and cmlc2:GFP as a transgenesis marker.DepositorTypeEmpty backboneUseCRISPR; Tol2 destination vector for gatewayTagsExpressionMutationPromoterAvailable sinceApril 13, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCIP-AMPKa1_KD
Plasmid#79011PurposeFor mammalian expression of a kinase dead (K47R) version of AMPK alpha1.DepositorInsertAMPK alpha 1 (PRKAA1 Human)
UseTagsExpressionMammalianMutationK47RPromoterpCAGGsAvailable sinceJuly 5, 2016AvailabilityAcademic Institutions and Nonprofits only -
pLenti-tetON-dxCas9(3.7)-VPR-EF1a-TagRFP-2A-tet3G
Plasmid#167937PurposeInducible CRISPRa expressionDepositorInsertdxCas9(3.7)-VPR
UseLentiviralTagsExpressionMutationPromotertetONAvailable sinceJune 7, 2021AvailabilityAcademic Institutions and Nonprofits only