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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 11783 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepT81Luc
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Backbone manufacturerATCC
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Vector typeBacterial Expression, Luciferase
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameNFAT/AP-1 3x
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Alt nameIL-2 promoter
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SpeciesM. musculus (mouse)
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Insert Size (bp)120
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Entrez GeneIl2 (a.k.a. Il-2)
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Tag
/ Fusion Protein
- luciferase (C terminal on backbone)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site HindIII (destroyed during cloning)
- 3′ cloning site XhoI (not destroyed)
- 5′ sequencing primer LucNrev (Common Sequencing Primers)
Resource Information
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A portion of this plasmid was derived from a plasmid made byDJ McKean (Hedin, et al., 1997, PMID 9548483)
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Articles Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Three tandem repeats of the NFAT/AP-1 binding site at approximately -287 of the murine IL-2 promotor. Sequence: 5'-AGCTTGGATCC AAGAGGAAAA TTTGTTTCAT ACAGAAGGCG TTAAGAGGAA AATTTGTTTC ATACAGAAGG CGTTAAGAGG AAAATTTGTT TCATACAGAA GGCGTTCAAG CTTGTCGAC-3
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
NFAT/AP-1 3x luciferase was a gift from Anjana Rao (Addgene plasmid # 11783 ; http://n2t.net/addgene:11783 ; RRID:Addgene_11783) -
For your References section:
Gene expression elicited by NFAT in the presence or absence of cooperative recruitment of Fos and Jun. Macian F, Garcia-Rodriguez C, Rao A. EMBO J. 2000 Sep 1. 19(17):4783-95. 10.1093/emboj/19.17.4783 PubMed 10970869