IL-2 promoter luciferase
(Plasmid #12194)

• Depositing Lab: Anjana Rao
• Publication: Macian et al EMBO J. 2000 Sep 1. 19(17):4783-95.

Backbone

• Vector backbone: pT81Luc
• Backbone manufacturer: ATCC
• Vector type: Bacterial Expression, Luciferase

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: IL-2 promoter
• Species: M. musculus (mouse)
• Insert Size (bp): 524
• Entrez Gene: Il2 (a.k.a. Il-2)
• Tag / Fusion Protein:
  • luciferase (C terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: SalI (not destroyed)
• 3′ cloning site: BglII (destroyed during cloning)
• 5′ sequencing primer: LucNrev

Resource Information

• A portion of this plasmid was derived from a plasmid made by: DJ McKean (Hedin, et al., 1997, PMID 9548483)
• Articles Citing this Plasmid:
  • 5 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Luciferase Limited Use Label License
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Murine IL-2 promotor from -585 to -34 relative to the transcription start site.