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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 10959 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepGL2-Basic
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Vector typeMammalian Expression, Luciferase
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameIL-2 promoter
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Alt nameIL-2
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SpeciesH. sapiens (human)
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Insert Size (bp)90
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Entrez GeneIL2 (a.k.a. IL-2, TCGF, lymphokine)
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Tag
/ Fusion Protein
- luciferase (C terminal on backbone)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ sequencing primer LucNRev (Common Sequencing Primers)
Resource Information
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Articles Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
A luciferase reporter containing three tandem repeats of a 30-bp fragment of the IL-2 promoter for analysis of NFAT activity. 30-bp fragment is GGAGG AAAAA CTGTT TCATA CAGAA GGCGT.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
NFAT luciferase reporter was a gift from Toren Finkel (Addgene plasmid # 10959 ; http://n2t.net/addgene:10959 ; RRID:Addgene_10959) -
For your References section:
Ras regulates NFAT3 activity in cardiac myocytes. Ichida M, Finkel T. J Biol Chem. 2001 Feb 2. 276(5):3524-30. 10.1074/jbc.M004275200 PubMed 11044444