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Plasmid#140190PurposeOverexpression of GFP with a nuclear localization sequence, under the human synapsin promoterDepositorInsertGFP-NLS
UseLentiviralTagsGFPExpressionMammalianMutationPromoterAvailable sinceJuly 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
pGEX-5X-1-LARP7
Plasmid#113545PurposeProduces an N-terminal GST-tagged full length LARP7 protein.DepositorInsertLARP7 (LARP7 Human)
UseTagsGSTExpressionBacterialMutationPromoterAvailable sinceJuly 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
mCh-Sec61 beta
Plasmid#49155Purposeexpression of Sec61 beta fused to mCherry, used as a general ER markerDepositorInsertSec61 beta (SEC61B Human)
UseTagsmCherry*ExpressionMammalianMutationPromoterCMVAvailable sinceNov. 20, 2013AvailabilityAcademic Institutions and Nonprofits only -
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pCRISPR-PE-bacteria
Plasmid#172715PurposeExpression of an E. coli codon optimized fusion protein of Cas9n-linker-M-MLV2 for prime editingDepositorInsertCas9n-linker-M-MLV2
UseTagsExpressionBacterialMutationH840A of Cas9PromoterAvailable sinceSept. 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
DR5rev::erRFP
Plasmid#61011PurposeAuxin signalling reporter (DR5rev) driving RFP (ER-targeted)DepositorInsertDR5rev::erRFP:35S_term
UseTagsExpressionPlantMutationPromoterDR5rev::erRFP:35StermAvailable sinceFeb. 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
pWZL hygro H-Ras V12
Plasmid#18749DepositorInsertH-Ras V12 (HRAS Human)
UseRetroviralTagsExpressionMammalianMutationV12PromoterAvailable sinceJuly 3, 2008AvailabilityAcademic Institutions and Nonprofits only -
T7-Elongin C-pcDNA3
Plasmid#19998DepositorInsertElongin C (ELOC Human)
UseTagsT7-tagExpressionMammalianMutationPromoterAvailable sinceApril 6, 2009AvailabilityAcademic Institutions and Nonprofits only -
pLAI-Env
Plasmid#133996PurposeEnv gene from HIV-1 LAI in pcDNA3.1 expression vectorDepositorInsertEnvelope Glycoprotein
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceMarch 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
Ai65(RCFL-tdT) targeting vector
Plasmid#61577PurposeTarget a Flp and Cre-dependent tdTomato expression cassette to the mouse Rosa26 locusDepositorInsertpCAG-FSF-LSL-tdTomato
UseCre/Lox and Mouse TargetingTagsExpressionMutationPromoterCAGAvailable sinceJune 30, 2015AvailabilityAcademic Institutions and Nonprofits only -
HRE-oUnaG
Plasmid#201712PurposeHypoxia responsive promoter driving UnaG fluorescent protein destablized using ODDD fragment from HIF1aDepositorInsertUnaG
UseTagsExpressionMammalianMutationPromoterAvailable sinceNov. 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
pDEST17-UbE2S
Plasmid#15807DepositorInsertUbiquitin conjugating enzyme UbE2S (UBE2S Human)
UseTagsHisExpressionBacterialMutationPromoterAvailable sinceSept. 28, 2007AvailabilityAcademic Institutions and Nonprofits only -
pLY017SB_pAAV-U6sg(BbsI)-EFS-Thy1.1-P2A-SB100X
Plasmid#192151PurposeT cell CRISPR AAV-SB vectorDepositorTypeEmpty backboneUseTagsExpressionMammalianMutationNAPromoterAvailable sinceNov. 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLGB36
Plasmid#135621PurposeSuicide vector for allelic replacement in Bacteroides species including B. fragilis strain 638R, erythromycin selection and aTC-inducible ss-Bfe3 counterselectionDepositorInsertbfe3
UseBacteroides suicide vector with inducible counter…Tagssignal sequence for periplasmic targetingExpressionMutationPromoterBacteroides promoter regualted by TetR transcript…Available sinceJan. 22, 2020AvailabilityAcademic Institutions and Nonprofits only -
HA-SOCS1
Plasmid#174571PurposeHuman HA-tagged SOCS1 expression (N-term. tag) (CMV prom.)DepositorInsertHA-SOCS1
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceJan. 19, 2023AvailabilityAcademic Institutions and Nonprofits only -
pK18msB
Plasmid#177839PurposePlasmid for gene replacement using kanamycin/sucrose selection/counterselection. Derived from pK18mobsacB but reduced in size.DepositorTypeEmpty backboneUseBacterial gene replacementTagsExpressionMutationPromoterAvailable sinceAug. 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
Proinsulin-NanoLuc in pLX304
Plasmid#62057PurposeLuminescent reporter of insulin secretionDepositorInsertProinsulin-NanoLuc (Ins2 Mouse, Synthetic)
UseLentiviral and LuciferaseTagsExpressionMammalianMutationInserted NanoLuc into C-peptide of mouse Ins2 cod…PromoterCMVAvailable sinceJan. 20, 2015AvailabilityAcademic Institutions and Nonprofits only -
pT7-PE7 for IVT
Plasmid#214813PurposeTemplate for in vitro transcription of PE7DepositorInsertPE7
UseTemplate for in vitro transcriptionTagsSV40 bpNLS and c-Myc NLSExpressionMutationPromoterT7 (inactivated)Available sinceMarch 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGWB414
Plasmid#74808PurposeGateway cloning compatible binary vector for C-terminal fusion with 3xHA (CaMV35S promoter).DepositorTypeEmpty backboneUseTagsExpressionPlantMutationPromoterCaMV35SAvailable sinceOct. 18, 2016AvailabilityAcademic Institutions and Nonprofits only -
pDESTmycYBX1
Plasmid#19878DepositorInsertY box binding protein 1 (YBX1 Human)
UseTagsMYCExpressionMammalianMutationPromoterAvailable sinceDec. 31, 2008AvailabilityAcademic Institutions and Nonprofits only -
pET-28a(+)-GDH
Plasmid#108954Purposeheterologous expression of GDH of B. megaterium in E. coliDepositorInsertGlucose dehydrogenase IV (GDH IV)
UseTagsHis6-TagExpressionBacterialMutationPromoterAvailable sinceDec. 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
CAG-GFP/cre
Plasmid#49054PurposeRetroviral vector contains CAG promoter , CMV enhancer and a large synthetic intron that used to allow the ubiquitous expression of GFP-fused Cre recombinaseDepositorInsertGFP/Cre
UseRetroviralTagsExpressionMammalianMutationPromoterCAGAvailable sinceNov. 4, 2013AvailabilityAcademic Institutions and Nonprofits only -
V1-STING
Plasmid#124263PurposeN-terminally tagged STING with split Venus Construct for studying localisation of STING in different cellular compartmentsDepositorInsertTMEM173 (STING1 Human)
UseTagsVenus1ExpressionMammalianMutationPromoterAvailable sinceMay 10, 2019AvailabilityAcademic Institutions and Nonprofits only -
Pet28a_Human_H4
Plasmid#42633DepositorInsertHuman H4 (H4C1 Human)
UseTagsUntaggedExpressionBacterialMutationPromoterT7Available sinceFeb. 25, 2013AvailabilityIndustry, Academic Institutions, and Nonprofits -
pHAGE-IRES-puro-NLS-dPguCas13b-3xEGFP-NLS-3xFlag
Plasmid#132399Purposeoverexpression in human cellsDepositorInsertdPguCas13b
UseLentiviralTagsflagExpressionMammalianMutationH151A; H1121APromoterAvailable sinceAug. 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET15b-mISG15(GG)
Plasmid#12448DepositorInsertC-terminal processed form of ISG15
UseTagsHisExpressionBacterialMutationPromoterAvailable sinceJan. 4, 2007AvailabilityAcademic Institutions and Nonprofits only -
pDEST17-UbE2L6
Plasmid#15797DepositorInsertUbiquitin conjugating enzyme UbE2L6 (UBE2L6 Human)
UseTagsHisExpressionBacterialMutationPromoterAvailable sinceSept. 28, 2007AvailabilityAcademic Institutions and Nonprofits only -
GFP-Rab7A
Plasmid#61803PurposeHuman Rab7A fluorescently tagged with GFP on the N-terminus for mammalian expressionDepositorInsertRab7A (RAB7A Human)
UseTags2x HA and GFPExpressionMammalianMutationNonePromoterCMVAvailable sinceFeb. 2, 2015AvailabilityAcademic Institutions and Nonprofits only -
5'UTR CGG 99x FMR1-FLAG
Plasmid#63090PurposeContains expanded CGG repeats (99 repeats) within the 5'UTR of FMR1 as found in the Fragile X Tremor Ataxia Syndrome (FXTAS). These repeats are in frame with a FLAG tag.DepositorInsertexpanded CGG repeats (99 repeats) within the 5'UTR of FMR1 (FMR1 Human)
UseTagsFLAGExpressionMammalianMutationPromoterCMVAvailable sinceApril 21, 2015AvailabilityAcademic Institutions and Nonprofits only -
pAAV-anti-PDL1(KN035) PAGER(TF)
Plasmid#230003PurposeExpresses anti-PDL1 PAGER(TF) in mammalian cells; used with NanoLuc-Arrestin-TEVp (Addgene #125228) and UAS-Firefly Luciferase reporter (Addgene #104840)DepositorInsertIL2SP-Aro6-KN035-TEVcs-ALFA-KORD(RAA)-LOV-TEVcs-Gal4
UseAAVTagsExpressionMammalianMutationV360A/R361A on KORDPromoterCMVAvailable sinceJan. 6, 2025AvailabilityAcademic Institutions and Nonprofits only -
pGP-AAV-syn-jGCaMP7f-WPRE
Plasmid#104488PurposeAAV-mediated expression of ultrasensitive protein calcium sensor under the Syn promoterDepositorHas ServiceAAV PHP.eB, AAV Retrograde, AAV1, AAV8, and AAV9InsertjGCaMP7f
UseAAVTagsT7 epitope, Xpress tag, 6xHisExpressionMammalianMutationPromoterSynapsinAvailable sinceDec. 13, 2017AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-2xFYVE
Plasmid#140047PurposeExpresses GFP-tagged HRS 2xFYVE in mammalian cellsDepositorInserttandem HRS FYVE domain (Hgs Mouse)
UseTagsEGFPExpressionMammalianMutationtwo FYVE domains (amino acids 147-223), linked by…PromoterCMVAvailable sinceMay 3, 2020AvailabilityAcademic Institutions and Nonprofits only -
pJYDC1
Plasmid#162458PurposeYeast display plasmid for expression of C-terminal fusions with Aga2p yeast agglutinin. The reporter, eUnaG2 bilirubin dependent green-yellow fluorescent protein, is fused to the NterminusDepositorTypeEmpty backboneUseShuttle vector bacteria/yeastTagsHA tag, HDEL sequence, eUnaG2 fluorescent protein…ExpressionMutationPromoterAvailable sinceFeb. 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
PM-RA
Plasmid#211705PurposeExpression of a dimerization-dependent fluorescent protein (ddFP) subunit (RA) fused with plasma membrane (PM) targetting motif of Lck in mammalian cellsDepositorInsertPM-targetted RA (Lck Synthetic)
UseTagsRAExpressionMammalianMutationPromoterCMVAvailable sinceJan. 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCR3-FLAG-Ubc13-WT
Plasmid#43938DepositorInsertubiquitin-conjugating enzyme E2N (UBE2N Human)
UseTags2x FLAGExpressionMammalianMutationPromoterAvailable sinceDec. 6, 2017AvailabilityAcademic Institutions and Nonprofits only -
pDONR221_SLC7A5
Plasmid#132197PurposeGateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectorsDepositorInsertSLC7A5 (SLC7A5 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceOct. 28, 2019AvailabilityIndustry, Academic Institutions, and Nonprofits -
AAV-Ef1a-InteinC-CreC
Plasmid#187615PurposeAAV vector encoding C-terminal portion of split-Intein-mediated split-CreDepositorInsertInteinC-CreC
UseAAVTagsExpressionMammalianMutationPromoterAvailable sinceJuly 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLKO.1 3xHA-KillerRed-OMP25
Plasmid#174544Purposelocalizes KillerRed to mitochondriaDepositorInsert3xHA-KillerRed-OMP25
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceAug. 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
pXL001-TQM IL13 CAR
Plasmid#154054PurposeLentiviral expression of TQM IL13 CAR for CAR-T therapyDepositorInsertTQM IL13 CAR
UseLentiviralTagsExpressionMutationPromoterEF1aAvailable sinceJuly 22, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAAV.TBG.PI.Null.bGH
Plasmid#105536Purposeempty AAV vector with TBG promoterDepositorHas ServiceAAV8InsertNone
UseAAVTagsExpressionMammalianMutationPromoterTBGAvailable sinceFeb. 2, 2018AvailabilityAcademic Institutions and Nonprofits only -
pMi(3xP3-EGFP)
Plasmid#102540PurposeTransgenesis vector based on the Minos transposon, carrying the 3xP3-EGFP markerDepositorInsertMinos transpoable element with 3xP3-EGFP marker
UseTagsExpressionMutationPromoterAvailable sinceMarch 30, 2018AvailabilityAcademic Institutions and Nonprofits only -
pGWB501
Plasmid#74843PurposeGateway cloning compatible binary vector for simple cloning of gene.DepositorTypeEmpty backboneUseTagsExpressionPlantMutationPromoterNo PromoterAvailable sinceAug. 3, 2016AvailabilityAcademic Institutions and Nonprofits only -
pc3.1-CAGGS-ASAP3-mCyRFP3
Plasmid#193323PurposeA red fluorescent protein with improved monomericity enables ratiometric voltage imaging with ASAP3DepositorInsertASAP3-R3
UseTagsExpressionMammalianMutationPromotercaggsAvailable sinceOct. 13, 2023AvailabilityAcademic Institutions and Nonprofits only -
pNCS mCherry
Plasmid#91769PurposeBacterial expression of a fluorescent protein, monomeric Cherry (mCherry)DepositorInsertmonomeric Cherry
UseTagsHexa-Histidine tag, Xpress epitope for detection …ExpressionBacterialMutationSee Depositor CommentsPromoterT7Available sinceJune 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCap-101-Hgy
Plasmid#227508PurposeCapture plasmid with hygromycin resistance and pIJ101 repliconDepositorTypeEmpty backboneUseTagsExpressionMutationPromoterAvailable sinceDec. 17, 2024AvailabilityAcademic Institutions and Nonprofits only -
pHR-pSFFV-dCas9-SunTag-P2A-BFP-dWPRE
Plasmid#122151PurposeExpresses dCas9-SunTag(24X) fusion and a fluorescent indicator BFP.DepositorInsertdCas9 fused to SunTag(24x)
UseLentiviralTags2xNLS-P2A-BFP and NLSExpressionMammalianMutationPromoterSFFVAvailable sinceMarch 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
pHR CD19-PI3K CAR
Plasmid#113018Purposefor lentivirus production of CD19-PI3K CARDepositorInsertCD19 CAR-P intracellular PI3k recruiting domain from CD19 (CD19 Synthetic, Human)
UseLentiviralTagsmCherryExpressionMammalianMutationPromoterAvailable sinceFeb. 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAG426GAL-ccdB
Plasmid#14155DepositorTypeEmpty backboneUseGateway destinationTagsExpressionYeastMutationPromoterAvailable sinceMarch 16, 2007AvailabilityAcademic Institutions and Nonprofits only -
CMV500 A-FOS
Plasmid#33353DepositorInsertFOS (Fos Mouse)
UseTagsFLAGExpressionMammalianMutationDominant Negative of c-Jun; heterodimerizes and i…PromoterCMVAvailable sinceDec. 19, 2011AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-HA-ROC2
Plasmid#19899DepositorInsertRING finger protein 2 (ROC2) (RNF7 Human)
UseTagsHAExpressionMammalianMutationPromoterAvailable sinceDec. 31, 2008AvailabilityAcademic Institutions and Nonprofits only