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pLKO_HA.HSV.Ollas_mCherry-NLS

Plasmid

#178259

Purpose

Nuclear Protein Barcode (nPC) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables spatial detection of CRISPR screens.

Depositor

Brian Brown

Article

Dhainaut et al Cell. 2022 Mar 10. pii: S0092-8674(22)00

Insert

nPC Tagged mCherry-NLS

Use

Lentiviral

Tags

HA.HSV.Ollas and Nuclear Localization Signal

Expression

Mutation

Promoter

eF1a

Available since

Jan. 26, 2022

Availability

Academic Institutions and Nonprofits only

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pLKO_HA.HSV.NWS_mCherry-NLS

Plasmid

#178258

Purpose

Nuclear Protein Barcode (nPC) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables spatial detection of CRISPR screens.

Depositor

Brian Brown

Article

Dhainaut et al Cell. 2022 Mar 10. pii: S0092-8674(22)00

Insert

nPC Tagged mCherry-NLS

Use

Lentiviral

Tags

HA.HSV.NWS and Nuclear Localization Signal

Expression

Mutation

Promoter

eF1a

Available since

Jan. 26, 2022

Availability

Academic Institutions and Nonprofits only

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AOI-WT-Cas9-sg-mouse Suz12-F2-GFP

Plasmid

#91882

Purpose

Expresses 3xFLAG-Cas9 and a gRNA targeting mouse Suz12

Depositor

Martine Roussel

Article

Vo et al Cell Rep. 2017 Mar 21;18(12):2907-2917.

Insert

gRNA targeting Suz12 (Suz12 Mouse)

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

June 26, 2017

Availability

Academic Institutions and Nonprofits only
pDONR221_SLC35F5

Plasmid

#132085

Purpose

Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

Depositor

Giulio Superti-Furga, RESOLUTE Consortium

Article

Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

Insert

SLC35F5 (SLC35F5 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 21, 2019

Availability

Industry, Academic Institutions, and Nonprofits

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pLKO.TRC1.shmNsd1.2, puro

Plasmid

#114447

Purpose

Lentiviral vector for expression of shRNA sequence targeting mouse Nsd1

Depositor

Adrian Bracken

Article

Streubel et al Mol Cell. 2018 Apr 19;70(2):371-379.e5.

Insert

shNsd1.2 (Nsd1 Mouse)

Use

Lentiviral and RNAi

Tags

Expression

Mutation

Promoter

Available since

Sept. 11, 2018

Availability

Academic Institutions and Nonprofits only

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pLKO.TRC1.shmNsd1.1, puro

Plasmid

#114446

Purpose

Lentiviral vector for expression of shRNA sequence targeting mouse Nsd1

Depositor

Adrian Bracken

Article

Streubel et al Mol Cell. 2018 Apr 19;70(2):371-379.e5.

Insert

shNsd1.1 (Nsd1 Mouse)

Use

Lentiviral and RNAi

Tags

Expression

Mutation

Promoter

Available since

Sept. 11, 2018

Availability

Academic Institutions and Nonprofits only

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pN1 VAMP2 R125TAG

Plasmid

#69877

Purpose

express in mammalian cells for unnatural amino acid incorporation into VAMP2

Depositor

Silvio Rizzoli

Article

Vreja et al ACS Nano. 2015 Nov 24;9(11):11034-41. do

Insert

VAMP2 (Vamp2 Rat)

Use

Synthetic Biology

Tags

Expression

Mammalian

Mutation

Amber and Ochre stop codons in the former linker …

Promoter

Available since

Jan. 25, 2016

Availability

Academic Institutions and Nonprofits only
pCAG-FLAG-IRES-blasticidin-Sox2 S248A

Plasmid

#182047

Purpose

expression of Sox2 mutants

Depositor

Hyonchol Jang

Article

Kim et al Exp Mol Med. 2021 Nov;53(11):1759-1768.

Insert

Sox2 (Sox2 Mouse)

Use

Tags

FLAG

Expression

Mammalian

Mutation

S248A

Promoter

CAG

Available since

June 6, 2022

Availability

Academic Institutions and Nonprofits only

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pCAG-FLAG-IRES-blasticidin-Sox2 S248A/T258A

Plasmid

#182049

Purpose

expression of Sox2 mutants

Depositor

Hyonchol Jang

Article

Kim et al Exp Mol Med. 2021 Nov;53(11):1759-1768.

Insert

Sox2 (Sox2 Mouse)

Use

Tags

FLAG

Expression

Mammalian

Mutation

S248A/T258A

Promoter

CAG

Available since

April 13, 2022

Availability

Academic Institutions and Nonprofits only

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pCAG-FLAG-IRES-blasticidin-Sox2 T258A

Plasmid

#182048

Purpose

expression of Sox2 mutants

Depositor

Hyonchol Jang

Article

Kim et al Exp Mol Med. 2021 Nov;53(11):1759-1768.

Insert

Sox2 (Sox2 Mouse)

Use

Tags

FLAG

Expression

Mammalian

Mutation

T258A

Promoter

CAG

Available since

April 13, 2022

Availability

Academic Institutions and Nonprofits only

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pCR3-Flag-AuroraB KD (K106R)

Plasmid

#108488

Purpose

expression of Flag-AuroraB kinase death (K106R)

Depositor

Susanne Lens

Article

Hengeveld et al Mol Cell Proteomics. 2012 May;11(5):47-5

Insert

AuroraB (AURKB Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

Kinase death K106A

Promoter

CMV

Available since

May 8, 2018

Availability

Academic Institutions and Nonprofits only

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pEGFP-YOD1-MutS2-C160S

Plasmid

#85668

Purpose

Expression of human YOD1 I292Q V295Q C160S mutant with N-terminal GFP tag

Depositor

Hemmo Meyer

Article

Papadopoulos et al EMBO J. 2016 Oct 17. pii: e201695148.

Insert

YOD1 (YOD1 Human)

Use

Tags

GFP

Expression

Mammalian

Mutation

C160S and I292Q and V295Q

Promoter

CMV

Available since

April 28, 2017

Availability

Academic Institutions and Nonprofits only

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pShuttle-EGFP-CLASP2 340-1362 9xS/A

Plasmid

#24373

Depositor

Torsten Wittmann

Article

Kumar et al J Cell Biol. 2009 Mar 23. 184(6):895-908

Insert

CLASP2 (340-1362) 9xS/A (CLASP2 Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Nonphosphorylatable CLASP2 deletion mutant. M…

Promoter

Available since

June 29, 2010

Availability

Academic Institutions and Nonprofits only

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pDONR221_SLC2A7

Plasmid

#132066

Purpose

Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

Depositor

Giulio Superti-Furga, RESOLUTE Consortium

Article

Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

Insert

SLC2A7 (SLC2A7 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 8, 2019

Availability

Industry, Academic Institutions, and Nonprofits

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pLKO.1_sh_ex13

Plasmid

#35165

Depositor

Benjamin Blencowe

Article

Gabut et al Cell. 2011 Sep 30;147(1):132-46. Epub 20

Insert

Foxp1 (Foxp1 Mouse)

Use

Lentiviral and RNAi

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 30, 2012

Availability

Academic Institutions and Nonprofits only
pDONR221_SLC35A3

Plasmid

#132135

Purpose

Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

Depositor

Giulio Superti-Furga, RESOLUTE Consortium

Article

Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

Insert

SLC35A3 (SLC35A3 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 4, 2019

Availability

Industry, Academic Institutions, and Nonprofits

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CMV-218_HNF4A2

Plasmid

#31090

Depositor

Gerhart Ryffel

Article

Senkel et al BMC Res Notes. 2009 . 2():210.

Insert

HNF4A (HNF4A Human)

Use

Flp/frt

Tags

myc

Expression

Mammalian

Mutation

splice variant 2 of HNF4A, deletion of CMV promo…

Promoter

Available since

Oct. 14, 2011

Availability

Academic Institutions and Nonprofits only

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pCS2+ cMyc-oDam_f_GFPoNLS

Plasmid

#85818

Purpose

iDamID plasmid. To express transiently the c-Myc-tagged and optimized E. coli Dam adenine methyltransferase fused to the nuclear-localized mmGFP via flexylinker.

Depositor

Joachim Wittbrodt

Article

Gutierrez-Triana et al Development. 2016 Oct 5. pii: dev.139261

Insert

cMyc oDam_f_GFPoNLS

Use

Tags

cMyc and oNLS (optimized nuclear localization sig…

Expression

Bacterial, Insect, Mammalia…

Mutation

L122A. Codon optimization compatible to work in M…

Promoter

Available since

Jan. 4, 2017

Availability

Academic Institutions and Nonprofits only

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pT5T_hp53_ST_CR2

Plasmid

#34923

Depositor

Thanos Halazonetis

Article

Petty et al EMBO J. 2011 Jun 1;30(11):2167-76. Epub

Insert

p53 DNA-binding (Res 94-358, deletion 293-321) (TP53 Human)

Use

Tags

Expression

Bacterial

Mutation

C135V, C141V, W146Y, C182S, V203A, R209P, C229Y, …

Promoter

T7

Available since

Feb. 22, 2012

Availability

Academic Institutions and Nonprofits only

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CMV-138_HNF4A8

Plasmid

#31093

Depositor

Gerhart Ryffel

Article

Senkel et al BMC Res Notes. 2009 . 2():210.

Insert

HNF4A (HNF4A Human)

Use

Flp/frt

Tags

myc

Expression

Mammalian

Mutation

splice variant 8 of HNF4A, deletion of CMV promo…

Promoter

Available since

Oct. 14, 2011

Availability

Academic Institutions and Nonprofits only

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