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iDamIDseq and iDEAR: An improved method and computational pipeline to profile chromatin-binding proteins.

Gutierrez-Triana JA, Mateo JL, Ibberson D, Ryu S, Wittbrodt J
Development. 2016 Oct 5. pii: dev.139261. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
85818pCS2+ cMyc-oDam_f_GFPoNLSiDamID plasmid. To express transiently the c-Myc-tagged and optimized E. coli Dam adenine methyltransferase fused to the nuclear-localized mmGFP via flexylinker.
85819pCS2+ oDam_f_GFPoNLSiDamID plasmid. To express transiently the optimized E. coli Dam adenine methyltransferase fused to the nuclear-localized mmGFP via flexylinker.
85820pCS2+ cMyc Dam_f_GFPoNLSiDamID plasmid. To express transiently the c-Myc-tagged , L122A-mutant E. coli Dam adenine methyltransferase fused to the nuclear-localized mmGFP via flexylinker.
85821pDestTol2 ZfUbi::cMyc-oDam_f_GFPoNLSiDamID plasmid. To stably and ubiquitously express the c-Myc-tagged and optimized E. coli Dam adenine methyltransferase fused to the nuclear-localized mmGFP via flexylinker.

Antibodies from Article