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Plasmid#133026PurposeExpresses EGFP-tagged tail anchor sequence of BikDepositorInsertBcl-2-interacting killer (BIK Human)
UseLentiviralTagsEGFPExpressionMammalianMutationdeleted amino acids 1-110PromoterHuman elongation factor 1 alphaAvailable sinceFeb. 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
MSCV N-term HA-FLAG puro BRD7 shRescue
Plasmid#41936DepositorInsertBRD7 (BRD7 Human)
UseRetroviralTagsFLAG and HAExpressionMammalianMutationPromoterAvailable sinceApril 1, 2013AvailabilityAcademic Institutions and Nonprofits only -
hPlekhm1-C/pIRES puro Glue (S778-A1056)
Plasmid#73456PurposeExpress human C-terminal part (Q778-A1056) Plekhm1 in mammalian cellsDepositorInsertPlekhm1 (PLEKHM1 Human)
UseTagsCBP tag, HA tag, and strep tagExpressionMammalianMutationPromoterAvailable sinceMarch 25, 2016AvailabilityAcademic Institutions and Nonprofits only -
nonphosphorylatable Cenp-B-targted Aurora B FRET sensor
Plasmid#45230DepositorInsertnonphos Cenp-B targeted Aurora B FRET sensor
UseTagsExpressionMammalianMutationt-a mutation in substrate sequencePromoterAvailable sinceJuly 16, 2013AvailabilityAcademic Institutions and Nonprofits only -
pLenti-VQRRA-P2A-GFP-PGK-Puro
Plasmid#110864PurposeLentiviral vector for constitutive expression of Cas9-VQRRA-P2A-GFP in mammalian cells (codon optimized)DepositorInsertCas9-VQRRA
UseLentiviralTagsFLAGExpressionMutationD1135V, R1335Q, T1337R and NLS sequence at the N-…PromoterEF1sAvailable sinceJune 27, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLenti-EF1a-Ly6G-P2A-PuroR-HA-miR30
Plasmid#163359PurposeLentiviral vector for negative control of knockdown expressing a non-target miR against Renilla luciferase, with expression of a Ly6G surface marker and a puromycin selection marker.DepositorInsertLy-6G (Ly6g Mouse)
UseLentiviral and RNAiTagsExpressionMammalianMutationPromoterEF1Available sinceJan. 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
CAG-xFNLS-T2A-EGFP-ires-puro
Plasmid#121171PurposeCAG promoter driven expression of xFNLS BE3 base editor, EGFP and Puromycin resistance.DepositorInsertxFNLS-T2A-EGFP-ires-puro
UseCRISPRTagsT2A-EGFP-ires-puroExpressionMammalianMutationPromoterAvailable sinceJuly 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
Opie2-NeoR[traF]+Opie2-PuroR[dsxM]
Plasmid#131618PurposeExpresses a functional NeoR only in females, a functional PuroR only in males, and dsRed in both genders.DepositorInsertsPuroR[dsxM]
NeoR[traF]
UseTagsExpressionInsectMutationPromoterOpie2Available sinceMay 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
pSUPER-retro-puro-tetO-Mek1-shRNA
Plasmid#53179PurposeExpresses shRNA against mouse Mek1 from puromycin resistance retroviral vectorDepositorInsertMEK1 (Map2k1 Mouse)
UseRNAi and RetroviralTagsExpressionMammalianMutationPromoterAvailable sinceJune 19, 2014AvailabilityAcademic Institutions and Nonprofits only -
MSCV-BRD9_BRG1BD-PGK-Puro-IRES-GFP
Plasmid#75121PurposeRetroviral expression plasmid encoding a human BRD9 bromodomain-swap mutant containing the BRG1 bromodomainDepositorInsertBRD9 (BRD9 Human)
UseRetroviralTagsExpressionMutationBromodomain replaced with the bromodomain of BRG1PromoterAvailable sinceJune 30, 2016AvailabilityAcademic Institutions and Nonprofits only -
MSCV-BRD9_BRD1BD-PGK-Puro-IRES-GFP
Plasmid#75119PurposeRetroviral expression plasmid encoding a human BRD9 bromodomain-swap mutant containing the BRD1 bromodomainDepositorInsertBRD9 (BRD9 Human)
UseRetroviralTagsExpressionMutationBromodomain replaced with the bromodomain of BRD1PromoterAvailable sinceMay 18, 2016AvailabilityAcademic Institutions and Nonprofits only -
pBabe 3XFLAG-S37A-GATA6-3XAU1 puro
Plasmid#72608PurposeFor mammalian expression of an N-terminally triple FLAG-tagged and C-terminally triple AU1-tagged full length human GATA6 with a single serine#37 to alanine mutationDepositorInsertGATA binding protein 6 (GATA6 Human)
UseRetroviralTags3XAU1 and 3XFLAGExpressionMammalianMutationchanged serine37 to alaninePromoterAvailable sinceJuly 12, 2016AvailabilityAcademic Institutions and Nonprofits only -
integrin beta6 Y762F, Y774F pBabe puro
Plasmid#13598DepositorInsertintegrin beta 6 Y762F Y774F (ITGB6 Human)
UseRetroviralTagsExpressionMammalianMutationY762F Y774FPromoterAvailable sinceJan. 8, 2007AvailabilityAcademic Institutions and Nonprofits only -
1070 pBabe puroL PTEN 360-364A A4
Plasmid#10943DepositorInsertPTEN (PTEN Human)
UseRetroviralTagsExpressionMammalianMutation360-364A (SSSTS to AAAAA); A4: S380A, T382A, T383…PromoterAvailable sinceJan. 3, 2006AvailabilityAcademic Institutions and Nonprofits only -
integrin alpha9 / alpha5 EGFP pBabe puro
Plasmid#13608DepositorInsertintegrin alpha 9 / alpha 5 (ITGA9 Human)
UseRetroviralTagsGFPExpressionMammalianMutationIntegrin alpha 9 chimera containing the cytoplasm…PromoterAvailable sinceJan. 8, 2007AvailabilityAcademic Institutions and Nonprofits only -
pLenti-VRERRA-P2A-GFP-PGK-Puro
Plasmid#110865PurposeLentiviral vector for constitutive expression of Cas9-VRERRA-P2A-GFP in mammalian cells (codon optimized)DepositorInsertCas9-VRERRA
UseLentiviralTagsFLAGExpressionMutationD1135V, G1218R, R1335E, T1337R, and NLS sequence …PromoterEF1sAvailable sinceJune 27, 2018AvailabilityAcademic Institutions and Nonprofits only -
MSCV-6NBC-GFP-PGK-Puro-Barcode83
Plasmid#83760Purposeindividual 6N barcode to make barcoded cell line variantsDepositorInsertBarcode83
UseRetroviralTagsExpressionMutationPromoterAvailable sinceNov. 8, 2016AvailabilityAcademic Institutions and Nonprofits only -
N112 nLV EF-1a-PGK-Puro Gal4-HP1a CS
Plasmid#43969DepositorInsertCbx5 (Cbx5 Mouse)
UseLentiviralTagsGal4/DBDExpressionMutationN-term chromo domain removedPromoterEF-1aAvailable sinceJune 5, 2013AvailabilityAcademic Institutions and Nonprofits only -
1038 pBabe puroL PTEN 360-364A
Plasmid#10940DepositorInsertPTEN (PTEN Human)
UseRetroviralTagsExpressionMammalianMutation360-364A (SSSTS to AAAAA)PromoterAvailable sinceJan. 3, 2006AvailabilityAcademic Institutions and Nonprofits only -
hPlekhm1-C/pIRES puro Glue (H987-end)
Plasmid#73458PurposeExpress human C-terminal part (H987-end) Plekhm1 in mammalian cellsDepositorInsertPlekhm1 (PLEKHM1 Human)
UseTagsCBP tag, HA tag, and strep tagExpressionMammalianMutationPromoterAvailable sinceMarch 14, 2016AvailabilityAcademic Institutions and Nonprofits only -
hPlekhm1-C/pIRES puro Glue (S778-Q986)
Plasmid#73457PurposeExpress human C-terminal part (S778-Q986) Plekhm1 in mammalian cellsDepositorInsertPlekhm1 (PLEKHM1 Human)
UseTagsCBP tag, HA tag, and strep tagExpressionMammalianMutationPromoterAvailable sinceMarch 14, 2016AvailabilityAcademic Institutions and Nonprofits only -
pAV-puro-U6+27-F30-Tornado-Acyclovir-IRE#A4
Plasmid#216823PurposeExpresses acyclovir-controllable IRE variant #A4 as circular RNA in mammalian cells using the Tornado expression system.DepositorInsertTornado-Acyclovir-IRE#A4
UseTagsExpressionMammalianMutationPromoterU6+27Available sinceAug. 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAV-puro-U6+27-F30-Tornado-Acyclovir-IRE#A8
Plasmid#216824PurposeExpresses acyclovir-controllable IRE variant #A8 as circular RNA in mammalian cells using the Tornado expression system.DepositorInsertTornado-Acyclovir-IRE#A8
UseTagsExpressionMammalianMutationPromoterU6+27Available sinceAug. 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAV-puro-U6+27-F30-Tornado-Acyclovir-IRE#A8-3
Plasmid#216825PurposeExpresses acyclovir-controllable IRE variant #A8-3 as circular RNA in mammalian cells using the Tornado expression system.DepositorInsertTornado-Acyclovir-IRE#A8-3
UseTagsExpressionMammalianMutationPromoterU6+27Available sinceAug. 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAV-puro-U6+27-F30-Tornado-Acyclovir-IRE#A8-4
Plasmid#216826PurposeExpresses acyclovir-controllable IRE variant #A8-4 as circular RNA in mammalian cells using the Tornado expression system.DepositorInsertTornado-Acyclovir-IRE#A8-4
UseTagsExpressionMammalianMutationPromoterU6+27Available sinceAug. 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCW-HA-hRb-WT-T373A-S608A-S612A-puro
Plasmid#212674PurposeExpress tagged hRb mutantDepositorInsertRb (RB1 Human)
UseLentiviralTagsHAExpressionMutationT373A/S608A/S612APromoterTREAvailable sinceAug. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
AAVS1-Puro cTnT CCND2-T2A-ChloC
Plasmid#179841Purposedonor plasmid for cardiac-specific CCND2-T2A-Chloc expression in human cellsDepositorInsertCCND2 (CCND2 Human)
UseCRISPR and TALEN; Donor plasmidTagschloride-conducting ChR (ChloC)ExpressionMammalianMutationPromoterTroponin T promoterAvailable sinceJuly 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
AAVS1-Puro cTnT CCND2-T2A-iChloC
Plasmid#179842Purposedonor plasmid for cardiac-specific CCND2-T2A-iChloc expression in human cellsDepositorInsertCCND2 (CCND2 Human)
UseCRISPR and TALEN; Donor plasmidTagsimproved chloride-conducting ChR (iChloC)ExpressionMammalianMutationPromoterTroponin T promoterAvailable sinceJuly 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
AAVS1-Puro cTnT CCND2-T2A-luciferase
Plasmid#179844Purposedonor plasmid for cardiac-specific CCND2-T2A-luciferase expression in human cellsDepositorInsertCCND2 (CCND2 Human)
UseCRISPR, Luciferase, and TALEN ; Donor plasmidTagsluciferaseExpressionMammalianMutationPromoterTroponin T promoterAvailable sinceJuly 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pPB-EF1A-Puro-mCherry-RAB7-S72A
Plasmid#221556PurposeExpresses Cherry-tagged human Rab7 with S72A mutation in mammalian cells. Compatible with piggybac transposase for genome integration.DepositorInsertRab7A (RAB7A Human)
UsePiggybacTagsmCherryExpressionMammalianMutationChange serine 72 to alaninePromoterEF1aAvailable sinceJuly 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
pPB-EF1A-Puro-hTBK1-E696K-EGFP
Plasmid#221554PurposeExpresses GFP-tagged human TBK1 with E696K mutation in mammalian cells. Compatible with piggybac transposase for genome integration.DepositorInsertTBK1 (TBK1 Human)
UsePiggybacTagsGFPExpressionMammalianMutationChanged glutamate 696 to lysinePromoterEF1aAvailable sinceJuly 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
p944-SWITCH-OVER insert: EF1a-Puro
Plasmid#217891Purposeinsert vector for Switch-OVER: Single loxP-STOP-EF1a-Puro-mU6DepositorTypeEmpty backboneUseCRISPR, Cre/Lox, and LentiviralTagsExpressionMammalianMutationPromoterhU6Available sinceMay 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
pKL038 Lenti U6 dCas12a gRNA Puro mCherry
Plasmid#195546PurposedCas12a gRNA expression backboneDepositorArticleInsertLenti U6- empty cassette_Direct repeat_puro_mcherry
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterU6Available sinceMay 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
pEF-IRES-puro6 +gal4DBDHIFCAD pGalO linker
Plasmid#207172Purposeexpression plasmid for Gal4DBD-HIF-CAD expression GalO linkerDepositorInsertGal4DBD-HIF1a-CAD GalO linker
UseTagsExpressionMammalianMutationPromoterEF1aAvailable sinceApril 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
SOX2-P2A-NLS-mClover3-IRES-Puro
Plasmid#215544PurposeDonor plasmid for knock-in P2A-NLS-mClover3 into the human SOX2 locusDepositorInsertSOX2 homologous recombination arms with IRES-Puro selection cassette (SOX2 Human)
UseCRISPRTagsExpressionMutationPromoterAvailable sinceMarch 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLenti-h.Rhno1 Cdk1mut7A-C-Myc-P2A-Puro
Plasmid#211617PurposeRhno1 Cdk1mut7ADepositorInserthRHNO1 (RHNO1 Human)
UseTagsExpressionMammalianMutationS58A, S98A, S133A, T159A, T198A, S191A, and T202APromoterCMVAvailable sinceJan. 22, 2024AvailabilityAcademic Institutions and Nonprofits only -
pKLV2.2-h7SKgRNA5(RUNX1(13))-hU6gRNA5(RUNX3(12))-PGKpuroBFP-W
Plasmid#208569PurposeLentiviral vector expressing gRNA targeting human RUNX1 and RUNX3DepositorUseLentiviralTagsExpressionMutationPromoterAvailable sinceJan. 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
pKLV2.2-h7SKgRNA5(RUNX1(13))-hU6gRNA5(RUNX3(13))-PGKpuroBFP-W
Plasmid#208570PurposeLentiviral vector expressing gRNA targeting human RUNX1 and RUNX3DepositorUseLentiviralTagsExpressionMutationPromoterAvailable sinceJan. 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
pKLV2.2-h7SKgRNA5(ARID1A(44))-hU6gRNA5(ARID1B(21))-PGKpuroBFP-W
Plasmid#200507PurposeLentiviral vector expressing gRNA targeting human ARID1A and ARID1BDepositorUseLentiviralTagsExpressionMutationPromoterAvailable sinceJan. 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
pKLV2.2-h7SKgRNA5(ARID1A(44))-hU6gRNA5(AAVS1)-PGKpuroBFP-W
Plasmid#200506PurposeLentiviral vector expressing gRNA targeting human ARID1A and AAVS1DepositorUseLentiviralTagsExpressionMutationPromoterAvailable sinceJan. 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
pKLV2.2-h7SKgRNA5(ARID1A(44))-hU6gRNA5(ARID1B(23))-PGKpuroBFP-W
Plasmid#200508PurposeLentiviral vector expressing gRNA targeting human ARID1A and ARID1BDepositorUseLentiviralTagsExpressionMutationPromoterAvailable sinceJan. 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
pKLV2.2-h7SKgRNA5(AAVS1)-hU6gRNA5(BbsI)-PGKpuroBFP-W
Plasmid#200502PurposeLentiviral vector expressing gRNA targeting human AAVS1DepositorInsertAAVS1 (AAVS1 Human)
UseLentiviralTagsExpressionMutationPromoterAvailable sinceJan. 17, 2024AvailabilityAcademic Institutions and Nonprofits only -
pKLV2.2-h7SKgRNA5(SMARCA4(43))-hU6gRNA5(AAVS1)-PGKpuroBFP-W
Plasmid#200503PurposeLentiviral vector expressing gRNA targeting human SMARCA4 and AAVS1DepositorUseLentiviralTagsExpressionMutationPromoterAvailable sinceJan. 17, 2024AvailabilityAcademic Institutions and Nonprofits only -
pKLV2.2-h7SKgRNA5(SMARCA4(43))-hU6gRNA5(SMARCA2(46))-PGKpuroBFP-W
Plasmid#200504PurposeLentiviral vector expressing gRNA targeting human SMARCA4 and SMARCA2DepositorUseLentiviralTagsExpressionMutationPromoterAvailable sinceJan. 17, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLenti_mpx_AsCas12a_h7SK(PacI)_U6(I-SceI)_PGK-puro
Plasmid#189633PurposeLentiviral expression of dual AsCas12a gRNAs for generating combinatorial AsCas12a 3Cs librariesDepositorInserth7SK Cas12a gRNA cassette, hU6 Cas12a gRNA cassette, PGK puro cassette
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterAvailable sinceJan. 17, 2024AvailabilityAcademic Institutions and Nonprofits only -
pKLV2.2-h7SKgRNA5(SMARCA4(43))-hU6gRNA5(SMARCA2(47))-PGKpuroBFP-W
Plasmid#200505PurposeLentiviral vector expressing gRNA targeting human SMARCA4 and SMARCA2DepositorUseLentiviralTagsExpressionMutationPromoterAvailable sinceJan. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
piggyBac_rtTA (4th_Gen)_SNCA_IRES_NGN2-2A-PURO_Synapsin-BirA (pEHA1623)
Plasmid#209085PurposeNgn2 mediated cortical neuron induction, SNCA(wt) expression, Synapsin promoter BirA ligase expressionDepositorUseTagsExpressionMammalianMutationhuman codon optimizedPromoterTRE4G and hSynAvailable sinceDec. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLenti_CHyMErA_U6(SpCas9_I-SceI)-(AsCas12_PacI)_PGK-puro
Plasmid#189634PurposeLentiviral expression of single SpCas9 and AsCas12a gRNAs for generating combinatorial CHyMErA 3Cs librariesDepositorInserthU6 Cas9-Cas12a gRNA cassette, PGK puro cassette
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterAvailable sinceNov. 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLenti_mpx_SpCas9_h7SK(PacI)_U6(I-SceI)_PGK-puro_v2
Plasmid#189632PurposeLentiviral expression of dual SpCas9 gRNAs for generating combinatorial SpCas9 3Cs librariesDepositorInserth7SK Cas9 gRNA cassette, hU6 Cas9 gRNA cassette, PGK puro cassette
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterAvailable sinceNov. 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
J135F_BattB_Vif(T27X4)-IRES-SpyTag-sfmCherry2(11)-PuroR
Plasmid#200637PurposeRecombination plasmid for Matreyek Bxb1(GT) landing pad in dual landing pad cellsDepositorInsertVif
UseTagsExpressionMammalianMutationT27stopPromoterAvailable sinceNov. 6, 2023AvailabilityAcademic Institutions and Nonprofits only