Skip to main content
Addgene

Enhanced single RNA imaging reveals dynamic gene expression in live animals.

Hu Y, Xu J, Gao E, Fan X, Wei J, Ye B, Xu S, Ma W
Elife. 2023 Mar 3;12:e82178. doi: 10.7554/eLife.82178. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
200763pSX2719Expresses mCherry fused MS2 coat protein and 24xGCN4 (Suntag) with the tissue-specific promoter semo-1 in C. elegans.
200764pSX2720Expresses Superfolder GFP-tag fused single change variable fragment antibody of GCN4 (Suntag) with the tissue-specific promoter col-19 in C. elegans.
200765pSX2739Expresses mKate2-tag fused CDC-42 with 8xMS2(V1) stem loops in C. elegans, and the tissue-specific promoter col-19 is used.
205157pcDNA-tdMCP-24xGCN4express tdMCP-24xGCN4
205158pcDNA-tdMCP-12xGCN4express tdMCP-12xGCN4
205159pcDNA-tdMCP-6xGCN4express tdMCP-6xGCN4
205160pcDNA-puro-TagBFP-β-ACTIN-3′UTR-8xMS2express β-ACTIN-3′UTR-8xMS2
205161pcDNA-puro-TagBFP-β-ACTIN-3′UTR-24xMS2express β-ACTIN-3′UTR-24xMS2
205162pcDNA-puro-kozak-scFv-GCN4-HAtag-msfGFP(V206K)-GBIexpress scFv-GCN4-HAtag-msfGFP(V206K)-GBI
205163pcDNA-tdMCP-msfGFP(V206K)-GBIexpress tdMCP-msfGFP(V206K)-GBI

Antibodies from Article