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Plasmid#141488PurposeLentiviral vector for overexpressing the SP5 transcription factor ORF with a unique 24-bp barcode. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertSP5 (SP5 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceJan. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
TFORF2094
Plasmid#141917PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertDEK (DEK Human)
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceJan. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
TFORF1638
Plasmid#141857PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertBNIP3 (BNIP3 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceJan. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
TFORF0297
Plasmid#141565PurposeLentiviral vector for overexpressing the CREM transcription factor ORF with a unique 24-bp barcode. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertCREM (CREM Human)
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceJan. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pEpicVector3G_Puro-T2A-EGFP-HA-FLAG-AU1
Plasmid#162098PurposeEGFP linked to 3 tags on the C-terminus as barcodes. Puromycin resistant.DepositorInsertPuromycin resistant; EGFP linked to HA-FLAG-AU1
UseLentiviralTagsHA-FLAG-AU1ExpressionMutationWTPromoterEF1AAvailable sinceSept. 30, 2022AvailabilityAcademic Institutions and Nonprofits only -
pEpicVector3mC_Puro-T2A-mCherry-VSVg-StrepTagII-ProtC
Plasmid#162099PurposemCherry linked to 3 tags on the C-terminus as barcodes. Puromycin resistant.DepositorInsertPuromycin resistant; mCherry linked to VSVg-StrepTagII-ProtC
UseLentiviralTagsVSVg-StrepTagII-ProtCExpressionMutationWTPromoterEF1AAvailable sinceSept. 30, 2022AvailabilityAcademic Institutions and Nonprofits only -
HNRNPA1_Halo_C_allele
Plasmid#178123PurposeDonor vector for endogenous tagging of human HNRNPA1 at the C-terminus with halotagInsertHalotag (HNRNPA1 Human)
UseDonor vectorTagsExpressionMutationPromoterAvailable sinceDec. 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
pT3TS-nCas9n-A71
Plasmid#125644Purposemicro-injection of poly-adenylated nCas9n RNADepositorInsertnCas9n
UseRna in vitro transcription vectorTagsExpressionMutationPromoterT3Available sinceJuly 15, 2019AvailabilityAcademic Institutions and Nonprofits only -
CSNK2A1 gRNA (BRDN0001145419)
Plasmid#76673Purpose3rd generation lentiviral gRNA plasmid targeting human CSNK2A1DepositorInsertCSNK2A1 (CSNK2A1 Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterhU6Available sinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
Lrrc4c-Fc-His
Plasmid#72085PurposeExpresses the extracellular region of the LRRC4C protein, C-terminally fused to the Fc region of human IgG1 + 6X histidine tag.DepositorInsertLrrc4c (Lrrc4c Mouse)
UseTagsFc-HisExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 26, 2016AvailabilityAcademic Institutions and Nonprofits only -
Lrrc4-Fc-His
Plasmid#72084PurposeExpresses the extracellular region of the LRRC4 protein, C-terminally fused to the Fc region of human IgG1 + 6X histidine tag.DepositorInsertLrrc4 (Lrrc4 Mouse)
UseTagsFc-HisExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 12, 2016AvailabilityAcademic Institutions and Nonprofits only -
mClY-N1
Plasmid#90457PurposeA YFP-based chloride sensor with enhanced chloride sensitivity, photostability and reduced pH interference. The sequence of mClY is EYFP-F46L/Q69K/H148Q/I152L/V163S/S175G/S205V/A206K.DepositorInsertmClY-N1
UseTagsExpressionMammalianMutationEYFP-F46L/Q69K/H148Q/I152L/V163S/S175G/S205V/A206…PromoterAvailable sinceMay 19, 2017AvailabilityAcademic Institutions and Nonprofits only -
pOG44_FLP_recombinase (pEHA1338)
Plasmid#209087PurposeFLP recombinase for FRT mediated integrationDepositorInsertFLP recombinase
UseTagsExpressionMammalianMutationPromoterAvailable sinceOct. 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLV-puro-N-3HA-SREBP1-C-Flag
Plasmid#134286PurposeLentivector encoding 3XHA (N-term) and Flag (C-term)-tagged SREBP1DepositorInsertSREBP1 (Srebf1 Mouse)
UseLentiviralTags3x HA and FlagExpressionMammalianMutationmouse SREBP1 isoform a precursorPromoterEF1aAvailable sinceMarch 19, 2020AvailabilityAcademic Institutions and Nonprofits only -
PB-TRE-dCas9-KRAB-MeCP2
Plasmid#122267PurposeSP-dCas9-KRAB-MeCP2 with doxycycline-inducible expressionDepositorInsertdCas9-KRAB-MeCP2
UseTagsdCas9-KRAB-MeCP2ExpressionMammalianMutationPromoterAvailable sinceFeb. 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
NES-mCherry-cPHx3
Plasmid#116862PurposePI(3,4)P2 biosensorDepositorInsertPLEKHA1 (PLEKHA1 Human)
UseTagsmCherryExpressionMammalianMutationamino acids 169-329 (tandem trimer)PromoterAvailable sinceOct. 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
pRK5-HA-Ubiquitin-K63
Plasmid#17606PurposeMammalian expression of HA tagged ubiquitin with K63 only, other lysines mutated to argininesDepositorInsertUbiquitin C (UBC Human)
UseTagsHAExpressionMammalianMutationK63 only, other lysines mutated to arginines. Enh…PromoterAvailable sinceMarch 31, 2008AvailabilityAcademic Institutions and Nonprofits only -
pCAG_iPE-N
Plasmid#214734Purposeencodes iPE-N prime editor (with MMLV-RT(H8Y, D200N, T306K, W313F, T330P, L603W) fused to the N terminus of nCas9-H840A) driven by a CAG promoterDepositorInsertCAG_iPE-N_bGH
UseCRISPR and Synthetic BiologyTagsExpressionMammalianMutationPromoterAvailable sinceMarch 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
rAAV2/MG1.2
Plasmid#184541PurposeAAV packaging plasmid, expressing Rep2 and AAV-MG1.2 capsidDepositorInsertAAV-MG1.2 capsid
UseAAV; Helper vector for packagingTagsExpressionMutationchanges on AAV9 capsid: K449R, Q588T; insertion o…PromoterAvailable sinceJuly 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET21b-Caspase-3
Plasmid#90087PurposeExpresses wild type caspase 3 in bacterial cellsDepositorInsertCaspase 3 (CASP3 Human)
UseTagsHis tagExpressionBacterialMutationWild typePromoterAvailable sinceMay 4, 2017AvailabilityAcademic Institutions and Nonprofits only -
pKLV-EF1amCherry-2A-OsTIR1-W
Plasmid#159296PurposeLentiviral vector expressing mCherry and OsTIR1 driven by human EF1a promoter for the auxin-inducible degron (AID) systemDepositorInsertOsTIR
UseLentiviralTagsExpressionMammalianMutationPromoterhuman EF1a promoterAvailable sinceSept. 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLenti-PGK-Hygro-PIP-FUCCI
Plasmid#138714PurposeLentiviral expression of the cell cycle marker PIP-FUCCIDepositorInsertPIP-FUCCI
UseLentiviralTagsmCherry and mVenusExpressionMammalianMutationPromoterhPGKAvailable sinceMay 8, 2020AvailabilityAcademic Institutions and Nonprofits only -
pRRL.SIN.EF1A.CD19-FMC63.218.CAR-3G
Plasmid#194458PurposeCAR featuring: GMCSF (sig. peptide); CD8A & CD8A (hinge & TM); CD28, 4-1BB & CD3ζ (signaling domains); anti-CD19 from FMC63 in VL-VH order and 218 linker (scFv). GFP-Zeo for selection and monitoring.DepositorInsertAnti-CD19 CAR
UseLentiviralTagsExpressionMutationPromoterAvailable sinceMarch 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
AAVS1-tdTomato
Plasmid#159275PurposeHuman AAVS1 targeting vector for knockin of a CAGGS promoter-driven tdTomato cassette (cloned between AgeI and PacI). Targeted cells will be puromycin resistant.DepositorInserttdTomato
UseAAV; S1 knockin donor vectorTagsExpressionMammalianMutationPromoterCAGGSAvailable sinceSept. 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
pHAGE-3FLAG-EEA1
Plasmid#176491PurposeFor lentiviral transduction of 3FLAG-EEA1DepositorInsertEEA1 (EEA1 Human)
UseLentiviralTags3xFLAGExpressionMutationPromoterAvailable sinceMay 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
tau/pET29b
Plasmid#16316DepositorInserttau (MAPT Human)
UseTagsExpressionBacterialMutationPromoterAvailable sinceDec. 21, 2007AvailabilityAcademic Institutions and Nonprofits only -
pRK5-HA-Ubiquitin-K48
Plasmid#17605PurposeMammalian expression of HA tagged ubiquitin with only K48, other lysines mutated to argininesDepositorInsertUbiquitin C (UBC Human)
UseTagsHAExpressionMammalianMutationK48 only, other lysines mutated to arginines. Enh…PromoterAvailable sinceMarch 31, 2008AvailabilityAcademic Institutions and Nonprofits only -
pSFV3
Plasmid#92072PurposeEmpty expression plasmid for production of recombinant genomic SFV RNA. Co-transfection with RNA from pSFV-Helper1 or pSFV-Helper2 will produce recombinant virus like particles.DepositorTypeEmpty backboneUseEmpty expression plasmid, for run-off transcripti…TagsExpressionMutationPromoterAvailable sinceJune 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAAVS1-TetOn-ZIM3-KRAB-dCas9-P2A-mCherry
Plasmid#212829PurposeDoxycycline-inducible (Zim3)KRAB-dCas9-HA-P2A-mCherry cassette for integration at the AAVS1-locus of the human genome.DepositorInsertZIM3-KRAB-dCas9-P2A-mCherry
UseTagsHA-tag and ZIM3-KRABExpressionBacterial and MammalianMutationPromoterCAG, TetOnAvailable sinceJune 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
TurboID-His6_pET21a
Plasmid#107177Purposeexpresses His6-tagged TurboID in bacteriaDepositorInsertTurboID (BirA mutant)
UseTagsExpressionBacterialMutationQ65P, I87V, R118S, E140K, Q141R, S150G, L151P, V1…PromoterAvailable sinceMay 4, 2018AvailabilityAcademic Institutions and Nonprofits only -
CAG-Cas9-T2A-EGFP-ires-puro
Plasmid#78311PurposeExpresses WT SpCas9, EGFP and Puromycin resistance from a CAG promoter.DepositorInsertWT SpCas9-T2A-EGFP-ires-puromycin resistance
UseCRISPRTagsT2A-EGFP-ires-puroExpressionMammalianMutationPromoterCAGAvailable sinceApril 12, 2017AvailabilityAcademic Institutions and Nonprofits only -
pTRIPZ shNS
Plasmid#127696PurposeDoxycyclin inducible pTRIPZ lentivirus vector containing a non-silencing sequence for both human and mouseDepositorInsertshNS
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceNov. 1, 2019AvailabilityAcademic Institutions and Nonprofits only -
CROP-seq-opti
Plasmid#106280PurposeA version of the CROP-seq plasmid as presented in Datlinger et al. that contains the sgRNA-(F+E)-combined optimized backbone for CRISPRi from Chen et al.DepositorInsertEF1a-Puro-WPRE-hU6-gRNA
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterU6Available sinceMarch 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
pmCherry-MS2coatProtein
Plasmid#103832PurposeLive cell reader for MS2 loop RNA productionDepositorInsertmCherry-MS2 coat protein (cp )
UseTagsExpressionMammalianMutationMCP: nt 202-240 are missing (13 aa), C242G (A81G)…PromoterCMVAvailable sinceJan. 9, 2018AvailabilityAcademic Institutions and Nonprofits only -
pBABE-puro-hTERT
Plasmid#1771PurposeRetroviral expression of hTERT. Used to create immortalized cell lines.DepositorInserthTERT (TERT Human)
UseRetroviralTagsExpressionMammalianMutationPromoterAvailable sinceJune 3, 2005AvailabilityAcademic Institutions and Nonprofits only -
U6-(BbsI)sgRNA_CAG-Venus-bpA
Plasmid#86985PurposeFor cloning and expression of sgRNA together with Venus expressionDepositorInsertVenus
UseTagsExpressionMammalianMutationVenus GFP variantPromoterCAGAvailable sinceApril 3, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCRIS-PITChv2-FBL
Plasmid#63672PurposePITCh donor vector for EGFP-2A-PuroR knock-in into human FBL locusDepositorInsertEGFP-2A-PuroR
UseCRISPRTagsExpressionMutationPromoterPromoterlessAvailable sinceDec. 18, 2015AvailabilityAcademic Institutions and Nonprofits only -
pLminP_Luc2P_RE1
Plasmid#90335PurposeNFkB - p50/p65 pathway gene reporterDepositorInsertFirefly luciferase
UseLentiviralTagsExpressionMutationPromoterNFKBAvailable sinceAug. 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pRMC2-msfGFP
Plasmid#194913PurposeTetracycline inducible expression of msfGFP in StaphylococciDepositorInsertShine Dalgarno Sequence followed by monomeric superfolder GFP
UseTetracycline-inducible expression vector for stap…TagsExpressionBacterialMutationPromoterAvailable sinceJuly 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
circRNA-synIRES-R25-NanoLuc
Plasmid#188116PurposeTemplate plasmid to transcribe circular RNA encoding NanoLucDepositorInsertNanoLuc
UseLuciferaseTagsExpressionMammalianMutationPromoterT7Available sinceOct. 14, 2022AvailabilityAcademic Institutions and Nonprofits only