Efficient gene knockout and genetic interaction screening using the in4mer CRISPR/Cas12a multiplex knockout platform.
Esmaeili Anvar N, Lin C, Ma X, Wilson LL, Steger R, Sangree AK, Colic M, Wang SH, Doench JG, Hart T
Nat Commun. 2024 Apr 27;15(1):3577. doi: 10.1038/s41467-024-47795-3.
(Link opens in a new window)
PubMed
(Link opens in a new window)
Article
Plasmids from Article
ID | Plasmid | Purpose |
---|---|---|
203398 | pRDA_550 | All-in-one lentiviral expression of gRNA and EnAsCas12a |
209551 | Inzolia library in pRDA_052 | The human, genome-wide, CRISPR knockout enAsCas12a gRNA library targets single genes and paralog pairs, triples, and quads with arrays of 4 gRNAs. |
209552 | Inzolia library in pRDA_550 | The human, genome-wide, CRISPR knockout enAsCas12a gRNA library targets single genes and paralog pairs, triples, and quads with arrays of 4 gRNAs. |