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Plasmid#155103Purposelentiviral plasmid expressing mClover3, Cas9 and a gRNA targeting LacZDepositorInsertLacZ_sgRNA_2
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterU6 promoterAvailable sinceAug. 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
LCV2_BFP2
Plasmid#155097PurposeLentiviral backbone for cloning and expression of U6 driven gRNAs with Esp3I/BsmBI cloning sites, puromycin selection and BFP2 expressionDepositorTypeEmpty backboneUseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterAvailable sinceAug. 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
LCV2_mCherry_LacZ_sgRNA
Plasmid#155108Purposelentiviral plasmid expressing mCherry, Cas9 and a gRNA targeting LacZDepositorInsertLacZ_sgRNA_2
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterU6 promoterAvailable sinceAug. 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
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pBOB-N-3HA-GK_v1
Plasmid#134275PurposeLentivector encoding 3XHA-tagged GK (isoform 1)DepositorInsertGk (Gk Mouse)
UseLentiviralTags3X HAExpressionMammalianMutation524 amino acids, isoform 1PromoterCMVAvailable sinceMay 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBS10-riboE-dddAI
Plasmid#186561PurposeRegulated expression of the DddA immunity determinant (DddAI) in FirmicutesDepositorInsertdddAI
UseTagsExpressionBacterialMutationPromoterpSpac(hy)Available sinceJuly 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pX458-Ef1a-Cas9-H2B-mCherry (dual gRNA: Ascl1 x2)
Plasmid#171099PurposeCRISPR/Cas9 expressing plasmid containing two gRNAs targeting mouse Ascl1.DepositorInserthSpCas9 (Ascl1 Mouse, S. pyogenes)
UseCRISPRTagsExpressionMutationPromoterEf1aAvailable sinceJune 28, 2021AvailabilityAcademic Institutions and Nonprofits only -
LCV2_mCherry_Luciferase_sgRNA
Plasmid#155109Purposelentiviral plasmid expressing mCherry, Cas9 and a gRNA targeting LuciferaseDepositorInsertLuciferase_sgRNA
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterU6 promoterAvailable sinceAug. 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
LCV2_LacZ_sgRNA_2
Plasmid#155093Purposelentiviral plasmid expressing Cas9 and gRNA targeting LacZDepositorInsertLacZ_sgRNA_2
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterU6 promoterAvailable sinceAug. 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
LCV2_Luciferase_sgRNA
Plasmid#155094Purposelentiviral plasmid expressing Cas9 and gRNA targeting LuciferaseDepositorInsertLuciferase_sgRNA
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterU6 promoterAvailable sinceAug. 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBOB-N-3HA-GK_v2
Plasmid#134277PurposeLentivector encoding 3XHA-tagged GK (isoform 2)DepositorInsertGk (Gk Mouse)
UseLentiviralTags3X HAExpressionMammalianMutation553 amino acids, isoform 2PromoterCMVAvailable sinceMay 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBOB-N-Flag-GK_v1
Plasmid#134270PurposeLentivector encoding Flag-tagged GK (isoform 1)DepositorInsertGk (Gk Mouse)
UseLentiviralTagsFlagExpressionMammalianMutationmouse Gk isoform 1; 524 amino acidsPromoterCMVAvailable sinceMay 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
pMBP-parallel1-GK5_v2 (D443N)
Plasmid#134293PurposeExpresses GK5 (isoform 2)-D443N mutant in bacteriaDepositorInsertGk5 (Gk5 Mouse)
UseTagsMBPExpressionBacterialMutationchanged Aspartic Acid 443 to AsparaginePromoterT7Available sinceMarch 20, 2020AvailabilityAcademic Institutions and Nonprofits only -
pMBP-parallel1-GK5_v2 (D280N)
Plasmid#134290PurposeExpresses GK5 (isoform 2)-D280N mutant in bacteriaDepositorInsertGk5 (Gk5 Mouse)
UseTagsMBPExpressionBacterialMutationchanged Aspartic Acid 280 to AsparaginePromoterT7Available sinceMarch 20, 2020AvailabilityAcademic Institutions and Nonprofits only -
pMBP-parallel1-GK5_v2 (D30N)
Plasmid#134289PurposeExpresses GK5 (isoform 2)-D30N mutant in bacteriaDepositorInsertGk5 (Gk5 Mouse)
UseTagsMBPExpressionBacterialMutationchanged Aspartic Acid 30 to AsparaginePromoterT7Available sinceMarch 20, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA6-C-Flag-GK5_v2
Plasmid#134282PurposeExpresses Flag-tagged GK5 (isoform 2) in mammalian cellsDepositorInsertGk5 (Gk5 Mouse)
UseTagsFlagExpressionMammalianMutationmouse Gk5 isoform 2; 534 amino acidsPromoterCMVAvailable sinceMarch 20, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA6-C-Flag-GK5_v1
Plasmid#134281PurposeExpresses Flag-tagged GK5 (isoform 1) in mammalian cellsDepositorInsertGk5 (Gk5 Mouse)
UseTagsFlagExpressionMammalianMutationmouse Gk5 isoform 1; 516 amino acidsPromoterCMVAvailable sinceMarch 20, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA6-GK5_v2
Plasmid#134280PurposeExpresses GK5 (isoform 2) in mammalian cellsDepositorInsertGk5 (Gk5 Mouse)
UseTagsExpressionMammalianMutationmouse Gk5 isoform 2; 534 amino acidsPromoterCMVAvailable sinceMarch 20, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA6-GK5_v1
Plasmid#134279PurposeExpresses GK5 (isoform 1) in mammalian cellsDepositorInsertGk5 (Gk5 Mouse)
UseTagsExpressionMammalianMutationmouse Gk5 isoform 1; 516 amino acidsPromoterCMVAvailable sinceMarch 20, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBOB-N-Flag-GK_v2
Plasmid#134271PurposeLentivector encoding Flag-tagged GK (isoform 2)DepositorInsertGk (Gk Mouse)
UseLentiviralTagsFlagExpressionMammalianMutationmouse Gk isoform 2; 553 amino acidsPromoterCMVAvailable sinceMarch 20, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBOB-C-3HA-GK5_v2
Plasmid#134269PurposeLentivector encoding 3XHA-tagged GK5 (isoform 2)DepositorInsertGk5 (Gk5 Mouse)
UseLentiviralTags3X HAExpressionMammalianMutationmouse Gk5 isoform 2; 534 amino acidsPromoterCMVAvailable sinceMarch 20, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBOB-C-3HA-GK5_v1
Plasmid#134268PurposeLentivector encoding 3XHA-tagged GK5 (isoform 1)DepositorInsertGk5 (Gk5 Mouse)
UseLentiviralTags3X HAExpressionMammalianMutationmouse Gk5 isoform 1; 516 amino acidsPromoterCMVAvailable sinceMarch 20, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBOB-C-Flag-GK5_v2
Plasmid#134260PurposeLentivector encoding Flag-tagged GK5 (isoform 2)DepositorInsertGk5 (Gk5 Mouse)
UseLentiviralTagsFlagExpressionMammalianMutationmouse Gk5 isoform 2; 534 amino acidsPromoterCMVAvailable sinceMarch 20, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBOB-C-Flag-GK5_v1
Plasmid#134257PurposeLentivector encoding Flag-tagged GK5 (isoform 1)DepositorInsertGk5 (Gk5 Mouse)
UseLentiviralTagsFlagExpressionMammalianMutationmouse Gk5 isoform 1; 516 amino acidsPromoterCMVAvailable sinceMarch 20, 2020AvailabilityAcademic Institutions and Nonprofits only -
pdCas9
Plasmid#46569PurposeExpresses the tracrRNA, the dCas9 catalytic site mutant and a CRISPR array designed for the easy cloning of new spacers.DepositorInsertstracrRNA
dcas9
CRISPR array
UseCRISPRTagsExpressionBacterialMutationD10A, H840APromoterAvailable sinceAug. 27, 2013AvailabilityAcademic Institutions and Nonprofits only -
pAG/MNase
Plasmid#123461PurposeExpresses Protein A and Protein G IgG binding domains fused to micrococcal nuclease for purposes of CUT&RUN. Includes (his)6 tag for purification and HA-tag for secondary immunoprecipitation.DepositorInsertProtein A-Protein G-Micrococcal Nuclease
UseTags6xHIS and HAExpressionBacterialMutationPromoterLacAvailable sinceMarch 21, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3.1-PPARA
Plasmid#169019PurposeExpression of PPARA driven by CMV promoterDepositorInserthuman ppara (PPARA Human)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceJune 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pRE57-Ter
Plasmid#170635PurposeDNA donor for transpositionDepositorInsertMini-Tn6677, Ter cassette
UseTagsExpressionBacterialMutationPromoterAvailable sinceAug. 4, 2021AvailabilityAcademic Institutions and Nonprofits only -
AAV.hSyn.GLPLight1
Plasmid#187466PurposeExpresses GLPLight1 in neuronsDepositorInsertGLPLight1
UseAAVTagsFlag tagExpressionMutationPromoterhuman Synapsin-1Available sinceFeb. 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
modified pcAT7-Glo1
Plasmid#160996PurposeSplicing reporter backboneDepositorInserthemoglobin subunit beta (HBB Human)
UseTagsExpressionMammalianMutationRepeated intron 1 with 40 nucelotide deletionPromoterCAGAvailable sinceJan. 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
AAV.hSyn.GLPLight-ctr
Plasmid#187468PurposeExpresses GLPLight-ctr in mammalian cellsDepositorInsertGLPLight-ctr
UseAAVTagsFlag tagExpressionMutationPromoterhuman Synapsin-1Available sinceFeb. 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pRha-tns
Plasmid#170630PurposeExpression of CRISPR-associated transposasesDepositorInsertVchTnsABC
UseTagsExpressionBacterialMutationPromoterAvailable sinceAug. 3, 2021AvailabilityAcademic Institutions and Nonprofits only -
CpxR-GFP
Plasmid#220150PurposeTo track the natural CpxR promoter's transcriptional dynamicsDepositorInsertCpxR promoter only
UseTagsExpressionBacterialMutationPromoterAvailable sinceAug. 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
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pRK5-HA GST RagD 121L
Plasmid#19309DepositorInsertRas-related GTP binding D (RRAGD Human)
UseTagsGST and HAExpressionMammalianMutationQ121LPromoterAvailable sinceSept. 15, 2008AvailabilityAcademic Institutions and Nonprofits only -
EF.CMV.RFP
Plasmid#17619DepositorHas ServiceCloning Grade DNATypeEmpty backboneUseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceMarch 27, 2008AvailabilityAcademic Institutions and Nonprofits only -
pRK5-HA GST RagD 77L
Plasmid#19308DepositorInsertRas-related GTP binding D (RRAGD Human)
UseTagsGST and HAExpressionMammalianMutationS77LPromoterAvailable sinceSept. 15, 2008AvailabilityAcademic Institutions and Nonprofits only -
pRK5-HA GST RagB 99L
Plasmid#19303DepositorInsertRas-related GTP binding B (RRAGB Human)
UseTagsGST and HAExpressionMammalianMutationQ99LPromoterAvailable sinceSept. 15, 2008AvailabilityAcademic Institutions and Nonprofits only -
pRK5-HA GST RagB 54L
Plasmid#19302DepositorInsertRas-related GTP binding B (RRAGB Human)
UseTagsGST and HAExpressionMammalianMutationT54LPromoterAvailable sinceSept. 15, 2008AvailabilityAcademic Institutions and Nonprofits only -
pRK5-HA GST RagC 75L
Plasmid#19305DepositorInsertRas-related GTP binding C (RRAGC Human)
UseTagsGST and HAExpressionMammalianMutationS75LPromoterAvailable sinceSept. 15, 2008AvailabilityAcademic Institutions and Nonprofits only -
pRK5-HA GST RagC 120L
Plasmid#19306DepositorInsertRas-related GTP binding C (RRAGC Human)
UseTagsGST and HAExpressionMammalianMutationQ120LPromoterAvailable sinceSept. 15, 2008AvailabilityAcademic Institutions and Nonprofits only -
pRK5-HA GST RagA 66L
Plasmid#19300DepositorInsertRas-related GTP binding A (RRAGA Human)
UseTagsGST and HAExpressionMammalianMutationQ66LPromoterAvailable sinceSept. 15, 2008AvailabilityAcademic Institutions and Nonprofits only -
pRK5-HA GST RagA 21L
Plasmid#19299DepositorInsertRas-related GTP binding A (RRAGA Human)
UseTagsGST and HAExpressionMammalianMutationT21LPromoterAvailable sinceSept. 15, 2008AvailabilityAcademic Institutions and Nonprofits only -
pMSCVpuro-Cas9-EGFP
Plasmid#159126PurposeExpresses active Cas9 fused with EGFP for fluorescence microscopyDepositorInsertCas9 fused to EGFP
UseCRISPR and RetroviralTagsEGFPExpressionMammalianMutationPromoterMSCV LTR promoterAvailable sinceDec. 14, 2020AvailabilityAcademic Institutions and Nonprofits only -
Lenti-EF1alpha-VP64-dCas9-VP64_Blast
Plasmid#192650Purpose3rd generation lenti vector encoding VP64-dCas9-VP64 with 2A Blast resistance markerDepositorInsertVP64-dCas9-VP64
UseCRISPR and LentiviralTagsExpressionMammalianMutationD10A and N863A in Cas9PromoterEF1aAvailable sinceDec. 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLV-hSyn-RFP
Plasmid#22909DepositorUseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceApril 8, 2010AvailabilityAcademic Institutions and Nonprofits only -
pCMV CEPIA2mt
Plasmid#58218PurposeGreen fluorescent indicator for calcium imaging in the mitochondriaDepositorInsertCEPIA2mt
UseTagsmycExpressionMammalianMutationGCaMP2 N263Y E282V M339LPromoterCMVAvailable sinceJuly 18, 2014AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1 3xF-GAR1
Plasmid#126873PurposeExpresses 3xFlag GAR1DepositorInsertGAR1 (GAR1 Human)
UseTags3xFlagExpressionMammalianMutationPromoterAvailable sinceAug. 12, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBluescript-Evf1
Plasmid#99479PurposeSubcloning of the Evf1 long non-coding RNADepositorInsertEvf1
UseTagsExpressionBacterialMutationPromoterAvailable sinceNov. 8, 2017AvailabilityAcademic Institutions and Nonprofits only