pHCD1C
(Plasmid
#102954)
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Purposegene cloning of human CD1C
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Depositing Labs
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 102954 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepCR2.1-TOPO
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Backbone manufacturerInvitrogen
- Backbone size w/o insert (bp) 3908
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Vector typeCloning
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameCD1C cDNA
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Alt nameNM_001765.2
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SpeciesH. sapiens (human)
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Insert Size (bp)1012
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Entrez GeneCD1C (a.k.a. BDCA1, CD1, CD1A, R7)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site XhoI (not destroyed)
- 3′ cloning site NotI (not destroyed)
- 5′ sequencing primer T7 (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pHCD1C was a gift from Gennaro De Libero & Lucia Mori (Addgene plasmid # 102954 ; http://n2t.net/addgene:102954 ; RRID:Addgene_102954) -
For your References section:
A novel self-lipid antigen targets human T cells against CD1c(+) leukemias. Lepore M, de Lalla C, Gundimeda SR, Gsellinger H, Consonni M, Garavaglia C, Sansano S, Piccolo F, Scelfo A, Haussinger D, Montagna D, Locatelli F, Bonini C, Bondanza A, Forcina A, Li Z, Ni G, Ciceri F, Jeno P, Xia C, Mori L, Dellabona P, Casorati G, De Libero G. J Exp Med. 2014 Jun 30;211(7):1363-77. doi: 10.1084/jem.20140410. Epub 2014 Jun 16. 10.1084/jem.20140410 PubMed 24935257