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Addgene

pHCD1C
(Plasmid #102954)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 102954 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pCR2.1-TOPO
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 3908
  • Vector type
    Cloning

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    CD1C cDNA
  • Alt name
    NM_001765.2
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1012
  • Entrez Gene
    CD1C (a.k.a. BDCA1, CD1, CD1A, R7)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site XhoI (not destroyed)
  • 3′ cloning site NotI (not destroyed)
  • 5′ sequencing primer T7
  • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pHCD1C was a gift from Gennaro De Libero & Lucia Mori (Addgene plasmid # 102954 ; http://n2t.net/addgene:102954 ; RRID:Addgene_102954)
  • For your References section:

    A novel self-lipid antigen targets human T cells against CD1c(+) leukemias. Lepore M, de Lalla C, Gundimeda SR, Gsellinger H, Consonni M, Garavaglia C, Sansano S, Piccolo F, Scelfo A, Haussinger D, Montagna D, Locatelli F, Bonini C, Bondanza A, Forcina A, Li Z, Ni G, Ciceri F, Jeno P, Xia C, Mori L, Dellabona P, Casorati G, De Libero G. J Exp Med. 2014 Jun 30;211(7):1363-77. doi: 10.1084/jem.20140410. Epub 2014 Jun 16. 10.1084/jem.20140410 PubMed 24935257