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PurposeExpresses the tracrRNA, the dCas9 catalytic site mutant and a CRISPR array designed for the easy cloning of new spacers.
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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 46569 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepACYC184
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Vector typeBacterial Expression, CRISPR
Growth in Bacteria
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Bacterial Resistance(s)Chloramphenicol, 25 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberLow Copy
Gene/Insert 1
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Gene/Insert nametracrRNA
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SpeciesS. pyogenes
Gene/Insert 2
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Gene/Insert namedcas9
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SpeciesS. pyogenes
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MutationD10A, H840A
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GenBank IDSpy_1046
Gene/Insert 3
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Gene/Insert nameCRISPR array
Resource Information
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A portion of this plasmid was derived from a plasmid made byThis plasmid was constructed by modifying the pCas9 plasmid constructed in the lab of Feng Zhang at MIT, and published in Nat Biotechnol. 2013 Mar;31(3):233-9. doi: 10.1038/nbt.2508. RNA-guided editing of bacterial genomes using CRISPR-Cas systems
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Articles Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pdCas9 was a gift from Luciano Marraffini (Addgene plasmid # 46569 ; http://n2t.net/addgene:46569 ; RRID:Addgene_46569) -
For your References section:
Programmable repression and activation of bacterial gene expression using an engineered CRISPR-Cas system. Bikard D, Jiang W, Samai P, Hochschild A, Zhang F, Marraffini LA. Nucleic Acids Res. 2013 Jun 12. 10.1093/nar/gkt520 PubMed 23761437